Category: Equipments
A Scalable Method to Study Neuronal Survival in Primary Neuronal Culture with Single-cell and Real-Time Resolution
Neuronal loss is on the core of many neuropathologies, together with stroke, Alzheimer’s illness, and Parkinson’s illness. Totally different strategies had been developed to review the method of neuronal survival upon cytotoxic stress. Most strategies are based mostly on biochemical approaches that don’t enable single-cell decision or contain advanced and expensive methodologies. Introduced here’s a…
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Characterization of Endogenous and Extruded H 2 S and Small Oxoacids of Sulfur (SOS) in Cell Cultures
This report characterizes and quantifies endogenous hydrogen sulfide (H2S) and small oxoacids of sulfur (SOS = HOSH, HOSOH) in a panel of cell strains together with human most cancers (A375 melanoma cells, HeLa cervical cells) and noncancer (HEK293 embryonic kidney cells), in addition to E. coli DH5α and S. cerevisiae S288C. The methodology used is a translation of well-studied…
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Development of a Pig Mammary Epithelial Cell Culture Model as a Non-Clinical Tool for Studying Epithelial Barrier-A Contribution from the IMI-ConcePTION Project
The ConcePTION mission goals at producing additional information in regards to the dangers associated to the usage of treatment throughout breastfeeding, as this data is missing for mostly used medication. Taking into account a number of points, the pig mannequin has been thought of by the consortium as essentially the most applicable selection. The current…
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In vitro OP9-DL1 co-culture and subsequent maturation in the presence of IL-21 generates tumor antigen-specific T cells with a favorable less-differentiated phenotype and enhanced functionality
T cell receptor (TCR)-redirected T cells goal intracellular antigens reminiscent of Wilms’ tumor 1 (WT1), a tumor-associated antigen overexpressed in a number of malignancies, together with acute myeloid leukemia (AML). For each chimeric antigen receptor (CAR)- and TCR-redirected T cells, a number of scientific research point out that T cell subsets with a less-differentiated phenotype…
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A Kinetic Pump Integrated Microfluidic Plate (KIM-Plate) with High Usability for Cell Culture-Based Multiorgan Microphysiological Systems
Microphysiological programs (MPSs), together with organ-on-a-chip (OoC), have attracted consideration as a novel technique for estimating the consequences and uncomfortable side effects of medication in drug discovery. To breed the dynamic in vivo atmosphere, earlier MPSs have been linked to pump programs to perfuse tradition medium. Due to this fact, most MPSs usually are not…
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The effect of macropore size of hydroxyapatite scaffold on the osteogenic differentiation of bone mesenchymal stem cells under perfusion culture
Earlier research have proved that dynamic tradition might facilitate vitamins transport and apply mechanical stimulation to the cells inside three-dimensional scaffolds, thus enhancing the differentiation of stem cells in the direction of the osteogenic phenotype. Nevertheless, the results of macropore measurement on osteogenic differentiation of stem cells underneath dynamic situation are nonetheless unclear. Subsequently, the…
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Electrochemical measurement of serotonin by Au-CNT electrodes fabricated on microporous cell culture membranes
Intestine-brain axis (GBA) communication depends on serotonin (5-HT) signaling between the intestine epithelium and the peripheral nervous system, the place 5-HT launch patterns from the basolateral (i.e., backside) aspect of the epithelium activate nerve afferents. There have been few quantitative research of this gut-neuron signaling because of an absence of real-time measurement instruments that may…
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Live-cell imaging of microglial interactions with radial glia in transgenic embryonic mouse brains using slice culture
Microglial dynamics and interactions with close by radial glia may be visualized in actual time in embryonic mouse mind tissue utilizing time-lapse imaging in slice tradition. This live-cell imaging protocol can be utilized to review the morphology and actions of a variety of cell varieties throughout quite a lot of mind areas and developmental time…
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Characterization of Endogenous and Extruded H 2 S and Small Oxoacids of Sulfur (SOS) in Cell Cultures
This report characterizes and quantifies endogenous hydrogen sulfide (H2S) and small oxoacids of sulfur (SOS = HOSH, HOSOH) in a panel of cell traces together with human most cancers (A375 melanoma cells, HeLa cervical cells) and noncancer (HEK293 embryonic kidney cells), in addition to E. coli DH5α and S. cerevisiae S288C. The methodology used is a translation of well-studied…
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3D microgroove electrical impedance sensing to examine 3D cell cultures for antineoplastic drug assessment
In latest a long time, three-dimensional (3D) most cancers cell fashions have attracted growing curiosity within the area of drug screening attributable to their vital benefits in additional correct simulations of heterogeneous tumor conduct in vivo in comparison with two-dimensional fashions. Moreover, drug sensitivity testing primarily based on 3D most cancers cell fashions can present…
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