
The effect of macropore size of hydroxyapatite scaffold on the osteogenic differentiation of bone mesenchymal stem cells under perfusion culture
Electrospun polyurethane/poly (ɛ-caprolactone) nanofibers promoted the attachment and progress of human endothelial cells in static and dynamic custom conditions
- On this study, the angiogenic functionality of human endothelial cells was studied after being plated on the ground of polyurethane-poly caprolactone (PU/PCL) scaffolds for 72 hours. On this study, cells have been designated into 5 fully completely different groups, along with PU, PU/PCL (2:1), PU/PCL (1:1); PU/PCL (1:2); and PCL.
- Info revealed that the PU/PCL (2:1) composition had the subsequent modulus and breakpoint as in contrast with the alternative groups (p<0.05). As compared with the alternative groups, the PU/PCL scaffold with a molar ratio of two:1 had lower the contact angle θ and higher tensile stress (p<0.05).
- The indicate measurement of the PU nanofibers was lowered after the addition of PCL (p<0.05). Based totally on our data, the custom of endothelial cells on the ground of PU/PCL (2:1) did not set off nitrosative stress and cytotoxic outcomes beneath static conditions as compared with cells plated on a standard plastic flooring (p>0.05).
- Based totally on data from the static scenario, we fabricated a tubular PU/PCL (2:1) assemble for six-day dynamic cell custom inside loop air-lift bioreactors. Scanning electron microscopy confirmed the attachment of endothelial cells to the luminal flooring of the PU/PCL scaffold. Cells have been flattened and aligned beneath the custom medium motion. Immunofluorescence imaging confirmed the attachment of cells to the luminal flooring indicated by blue nuclei on the luminal flooring.
- These data demonstrated that the equipment of PU/PCL substrate could stimulate endothelial cells train beneath static and dynamic conditions.
Three-Dimensional CellCultures as an In Vitro Gadget for Prostate Most cancers Modeling and Drug Discovery
Inside the last decade, three-dimensional (3D) cell custom know-how has gained a variety of curiosity attributable to its potential to larger recapitulate the in vivo group and microenvironment of in vitro cultured most cancers cells.
Significantly, 3D tumor fashions have demonstrated a variety of fully completely different traits in distinction with typical two-dimensional (2D) cultures and have equipped an attention-grabbing hyperlink between the latter and animal experiments.
Definitely, 3D cell cultures characterize a useful platform for the identification of the natural choices of most cancers cells along with for the screening of novel antitumor brokers. The present consider is geared towards summarizing the commonest 3D cell custom methods and functions, with a consider prostate most cancers modeling and drug discovery.
Temperature responsive methylcellulose-hyaluronic hydrogel as a 3D cellculture matrix
- This study investigated the equipment of a temperature-responsive methylcellulose-hyaluronic acid (MC-HA) hydrogel to help 3D cell progress in vitro. Preliminary work centered on the preparation of hydrogels for 3D custom, adopted by investigations of the natural compatibility of hydrogel elements and optimisation of the cell custom environment.
- Evaluation of viability and proliferation of HCT116 cells cultured inside the MC-HA hydrogel was used to control the combination composition so to design a hydrogel with optimum properties to help cell progress.
- Two important factors in relation to utility of the proposed polymeric matrix in 3D cell custom have been demonstrated: i) 3D cultured cell aggregates will likely be launched/recovered from the matrix by means of a fragile course of that may shield cell viability, and ii) the hydrogel matrix is amenable to utility in 96-well plate format as an everyday methodology employed in in vitro tissue custom exams.
- The work on account of this truth reveals that MC-HA hydrogels present potential for in vitro 3D cell custom as low-cost and well-defined alternate choices to animal-derived or difficult synthetic strategies.

Mouse Primordial Germ Cells: In Vitro Custom and Conversion to Pluripotent Stem Cell Traces
Primordial germ cells (PGCs) are the embryonic precursors of the gametes. No matter a very long time of research, in vitro custom of PGCs stays a major problem and has beforehand relied on undefined elements akin to serum and feeders.
Notably, PGCs cultured for extended intervals do not preserve their lineage id nonetheless as an alternative bear conversion to variety pluripotent stem cell strains referred to as embryonic germ (EG) cells in response to LIF/STAT3 signaling. Proper right here we report every established and new methodologies to derive EG cells, in a wide range of varied conditions.
We current that major fibroblast progress subject simply is not required for EG cell conversion. We component the steps taken in our laboratory to systematically take away difficult elements and arrange a very outlined protocol that allows surroundings pleasant conversion of isolated PGCs to pluripotent EG cells.
In addition to, we present that PGCs can adhere and proliferate in custom with out the help of feeder cells or serum. This may successfully counsel novel approaches to establishing short-term custom of PGCs in outlined conditions.
![]() Human Cytokine Primer Library II |
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HCA-II | Real Time Primers | 1 set | EUR 450 |
![]() Rat Cytokine Primer Library II |
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RCA-II | Real Time Primers | 1 set | EUR 548 |
![]() Mouse Cytokine Primer Library II |
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MCA-II | Real Time Primers | 1 set | EUR 450 |
![]() Human Colon Cancer Primer Library II |
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HCCP-II | Real Time Primers | 1 set | EUR 548 |
![]() Human DNA Repair Primer Library II |
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HDRL-II | Real Time Primers | 1 set | EUR 548 |
![]() Mouse DNA Repair Primer Library II |
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MDRL-II | Real Time Primers | 1 set | EUR 645 |
![]() Human Cancer Driver Gene II Primer Library |
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HCDG-II | Real Time Primers | 1 set | EUR 645 |
![]() Human Interferon Type II Signaling Primer Library |
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HIFN-II | Real Time Primers | 1 set | EUR 548 |
![]() A Medium |
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DJ1018 | Bio Basic | 100g | EUR 84.8 |
![]() Advanced Medium |
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C0003-04 | Addexbio | RT 500 mL Bottle | EUR 103 |
![]() Medium 199 |
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C0012-01 | Addexbio | RT 500 mL Bottle | EUR 105 |
![]() Heller's Medium |
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CP014-010 | GenDepot | 10X1L | EUR 99 |
![]() Heller's Medium |
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CP014-500 | GenDepot | 50L | EUR 126 |
![]() Hoagland's Medium |
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CP015-010 | GenDepot | 10X1L | EUR 99 |
![]() Hoagland's Medium |
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CP015-500 | GenDepot | 50L | EUR 126 |
![]() M9 Medium |
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SD7024 | Bio Basic | 250g | EUR 71.75 |
![]() M9CA Medium |
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SD7025 | Bio Basic | 250g | EUR 71.75 |
![]() YM Medium |
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SD7031 | Bio Basic | 250g | EUR 70.45 |
![]() TYGPN Medium |
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SD7032 | Bio Basic | 250g | EUR 70.01 |
![]() M63 Medium |
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SD7033 | Bio Basic | 250g | EUR 71.75 |
![]() NeuroProgenitor Medium |
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NM42400 | Neuromics | 125 ml | EUR 304 |
![]() COLIFORM MEDIUM |
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C03-127-10kg | Alphabiosciences | 10 kg | EUR 1324 |
![]() COLIFORM MEDIUM |
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C03-127-2kg | Alphabiosciences | 2kg | EUR 327 |
![]() COLIFORM MEDIUM |
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C03-127-500g | Alphabiosciences | 500 g | EUR 125 |
![]() HLP MEDIUM |
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H08-107-10kg | Alphabiosciences | 10 kg | EUR 2278 |
![]() HLP MEDIUM |
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H08-107-2Kg | Alphabiosciences | 2 Kg | EUR 534 |
![]() HLP MEDIUM |
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H08-107-500g | Alphabiosciences | 500 g | EUR 182 |
![]() EC MEDIUM |
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E05-100-10kg | Alphabiosciences | 10 kg | EUR 814 |
![]() EC MEDIUM |
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E05-100-2Kg | Alphabiosciences | 2 Kg | EUR 216 |
![]() EC MEDIUM |
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E05-100-500g | Alphabiosciences | 500 g | EUR 95 |
![]() DC MEDIUM |
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D04-117-10kg | Alphabiosciences | 10 kg | EUR 1579 |
![]() DC MEDIUM |
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D04-117-2kg | Alphabiosciences | 2kg | EUR 382 |
![]() DC MEDIUM |
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D04-117-500g | Alphabiosciences | 500 g | EUR 140 |
![]() SIM MEDIUM |
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S19-110-10kg | Alphabiosciences | 10 kg | EUR 1021 |
![]() SIM MEDIUM |
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S19-110-2kg | Alphabiosciences | 2kg | EUR 261 |
![]() SIM MEDIUM |
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S19-110-500g | Alphabiosciences | 500 g | EUR 107 |
![]() SOB MEDIUM |
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S19-124-10kg | Alphabiosciences | 10 kg | EUR 965 |
![]() SOB MEDIUM |
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S19-124-2kg | Alphabiosciences | 2kg | EUR 249 |
![]() SOB MEDIUM |
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S19-124-500g | Alphabiosciences | 500 g | EUR 104 |
![]() Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti rabbit secondary antibody |
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K3171120-II | Biochain | 1200 cm2 | EUR 246 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
![]() Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti rabbit secondary antibody |
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K3171250-II | Biochain | 2500 cm2 | EUR 310 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
![]() Aligner 13-24 |
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KS072012-II | Biochain | 1 Kit | EUR 412 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
![]() RapidSeq High Yield Directional mRNA Sample Prep Kit - With Aligner 13-24 |
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KS073012-II | Biochain | 1 Kit | EUR 1142 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
![]() RapidSeq High Yield Small RNA Sample Prep Kit - With Aligner 13-24 |
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KS074012-II | Biochain | 1 Kit | EUR 1021 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
![]() Rat Lung PrimaCell 7: Normal Alveolar Epithelial Cells II Growth Medium |
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9-25096 | CHI Scientific | 5 x 100 ml | Ask for price |
![]() Mouse Lung PrimaCell 7: Normal Alveolar Epithelial Cells II Growth Medium |
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9-32101 | CHI Scientific | 5 x 100 ml | Ask for price |
![]() Human Lung PrimaCell 7: Normal Alveolar Epithelial Cells II Growth Medium |
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9-46122 | CHI Scientific | 5 x 100 ml | Ask for price |
![]() NATtrol Chlamydia trachomatis LGV II 434, External Run Control, Medium (6X1mL) |
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NATCT(434)-ERCM | Zeptometrix | 6X1mL | EUR 401.52 |
Description: Please contact Gentaur in order to receive the datasheet of the product. |
![]() 50ml TC Tubes, Conical, 440 units/box |
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04-5540150 | Gentaur Genprice | 440 units/box | EUR 71 |
![]() GMP IL4, 50µg |
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04-GMP-HU-IL4-50UG | Gentaur Genprice | 50µg | EUR 483 |
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques. |
![]() SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE |
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04-GSB | Gentaur Genprice |
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Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You! |
![]() rHu IL 2 , 3MIU |
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04-RHIL2-02F01 | Gentaur Genprice | 1 vial | EUR 249 |
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated. |
![]() rHu IL 2 , 3MIU , Lot 200908F02 |
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04-RHIL2-08F02 | Gentaur Genprice | 1 vial | EUR 249 |
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated. |
![]() PRE-GMP rHu GM-CSF, Molgramostim-Leukoma |
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04-RHUGM-CSF-7A10 | Gentaur Genprice | 300 µg | EUR 385 |
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD. |
![]() Insect Cell Medium: TNM-FH Insect Culture Medium |
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ABP-MED-10001 | Allele Biotech | 1 liter | Ask for price |
![]() Insect Cell Medium: Serum-Free Insect Culture Medium |
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ABP-MED-10002 | Allele Biotech | 1 liter | Ask for price |
![]() Insect Cell Medium: Grace?s Insect Medium (Unsupplemented) |
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ABP-MED-10004 | Allele Biotech | 1 liter | Ask for price |
![]() H Medium Broth |
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DJ1019 | Bio Basic | 100g | EUR 60.88 |
![]() Aqueous Mounting Medium |
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AR1018 | BosterBio | 10mL X5 (Enough for 800-1200 assays) | EUR 80 |
![]() Antifade Mounting Medium |
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AR1109 | BosterBio | 10mL (for 1000 assays) | EUR 65 |
![]() AddexBio Cryopreservation Medium |
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C0002-20mL | Addexbio | RT vial | EUR 104 |
![]() RPMI 1640 Medium |
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abx098879-500ml | Abbexa | 500 ml | EUR 175 |
![]() Recovery Medium - 6x12mL |
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1711 | Intact Genomics | 4/PK | EUR 128 |
![]() Agarose, medium EEO |
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GE4750-100G | Glentham Life Sciences | 100 g | EUR 166 |
![]() Agarose, medium EEO |
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GE4750-500G | Glentham Life Sciences | 500 g | EUR 429 |
![]() Schwann Growth Medium |
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SGM001 | Neuromics | 500 ml | EUR 244 |
![]() MCDB 131 Medium |
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CM034-050 | GenDepot | 500ml | EUR 85 |
![]() MCDB 131 Medium |
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CM034-300 | GenDepot | 6x500ml | EUR 165 |
![]() MCDB 131 Medium |
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CM034-310 | GenDepot | 10x500ml | EUR 219 |
![]() MCDB 131 Medium |
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CM034-320 | GenDepot | 20x500ml | EUR 340 |
![]() MCDB 131 Medium |
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CM034-350 | GenDepot | 50x500ml | EUR 585 |
![]() Williams' Medium E |
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CM063-050 | GenDepot | 500ml | EUR 91 |
![]() Williams' Medium E |
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CM063-300 | GenDepot | 6x500ml | EUR 266 |
![]() Williams' Medium E |
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CM063-310 | GenDepot | 10x500ml | EUR 313 |
![]() Williams' Medium E |
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CM063-320 | GenDepot | 20x500ml | EUR 604 |
![]() Williams' Medium E |
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CM063-350 | GenDepot | 50x500ml | EUR 975 |
![]() Cereal Crops Medium |
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CP003-005 | GenDepot | 5L | EUR 113 |
![]() Cereal Crops Medium |
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CP003-010 | GenDepot | 10X1L | EUR 169 |
![]() Kao Michayluk Medium |
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CP017-010 | GenDepot | 10X1L | EUR 99 |
![]() Kao Michayluk Medium |
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CP017-500 | GenDepot | 50L | EUR 126 |