Ultra Rainbow

Ultra Rainbow Fluorescent Particles SPHERO

 

Motion Cytometry Alignment Particles

Spherotech offers every kind of particles for determining if a motion cytometer is appropriately aligned and has a transparent motion cell with no fluidic blockage. Using SPHERO Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions could also be measured to seek out out the alignment and efficiency of the motion cytometer. Additiona information could also be found at STN-8: Calibration and Effectivity Monitoring of Motion Cytometer Using SPHERO Calibration Particles.

Spherotech Cat. No. URFP-30-2 Instructions for use:
A. Preparation of Particles
1. Vortex the particles vigorously
2. Add 2 to 4 drops of particles to 1mL of sheath fluid or DI water. The inclusion of a small amount of detergent (~0.01%) inside the dilution buffer will enhance the proportion of the singlet inhabitants.

B. Every day Alignment
To search out out the optical alignment of the system perform the following:
1. Set a keep gate for the singlet inhabitants on the FSC vs SSC histogram to exclude aggregates
2. Modify the Purchase and Extreme voltage so that the suggest channel number of the peak is in a predetermined place on each histogram of curiosity. The histograms on the sooner internet web page will be utilized as a info.
3. Accumulate 5000 events contained within the gate
4. Report the % CV and Extreme Voltage for all fluorescence channels of curiosity.
5. Use a computer program resembling Excel to generate the Levy Jennings graphs.

NOTE: If the values on any parameter exceed these of the day-to-day frequent or preset values, which can be determined by not lower than one months worth of knowledge, additional calibration or alignment procedures have to be carried out in response to the instrument operation handbook.

 

 

Ultra Rainbow
Extremely Rainbow
SPHERO™ Extraordinarily Rainbow Fluorescent Particles – A single inhabitants of Rainbow Fluorescent Particles with enhanced UV and Far Pink fluorescence depth
Catalog No.
Description
Focus
Nominal Dimension
Bundle Dimension
Price
URFP-02-2
Extraordinarily Rainbow Fluorescent Particles
1010/mL
0.1-0.Three µm
2 mL
$250.00
URFP-05-2
Extraordinarily Rainbow Fluorescent Particles
107/mL
0.4-0.6 µm
2 mL
$250.00
URFP-10-5
Extraordinarily Rainbow Fluorescent Particles
107/mL
1.0-1.4 µm
5 mL
$250.00
URFP-30-2
Extraordinarily Rainbow Fluorescent Particles
107/mL
3.0-3.4 µm
2 mL
$250.00
URFP-30-20
Extraordinarily Rainbow Fluorescent Particles
107/mL
3.0-3.4 µm
20 mL
$2000.00
URFP01-30-2KExtraordinarily Rainbow Fluorescent Particles106/mL3.0-3.4 µm2×15 mL$355.00
URFP01-30-10OkayExtraordinarily Rainbow Fluorescent Particles106/mL3.0-3.4 µm10×3 mL$410.00
URFP-38-2
Extraordinarily Rainbow Fluorescent Particles
107/mL
3.5-3.9 µm
2 mL
$255.00
URFP-38-10
Extraordinarily Rainbow Fluorescent Particles
107/mL
3.5-3.9 µm
10 mL
$1000.00
URFP-38-5A
Extraordinarily Rainbow Fluorescent Particles, Mid-Fluctuate
107/mL
3.5-3.9 µm
5 mL
$565.00
URFP-100-2
Extraordinarily Rainbow Fluorescent Particles
107/mL
8.1-12.Zero µm
2 mL
$285.00
URFP-150-2
Extraordinarily Rainbow Fluorescent Particles
5×106/mL
13.0-17.9 µm
2 mL
$285.00
URFP-200-5
Extraordinarily Rainbow Fluorescent Particles
1.0% w/v
18.0-24.9 µm
5 mL
$290.00
URFP-300-5
Extraordinarily Rainbow Fluorescent Particles
1.0% w/v
25.0-35.Zero µm
5 mL
$290.00

 

Description
Extraordinarily Rainbow Fluorescent Particles (by Spherotech™) are internally labeled with quite a lot of fluorophores. They exhibit full spectrum fluorescence, along with enhanced UV and Far Pink fluorescence. The mixture of fluorophores permits Extraordinarily Rainbow particles to be excited at any wavelength from 365 to 650nm. These particles will be present in two diameters, ~3.8µm and ~10.2µm.

Traits
Suggest Diameter: ~3.8µm (3.61-3.99µm) or ~10.2µm (8.1 – 12.0µm); see your Certificates of Analysis for exact measurement.
Focus: ~2 x 106particles/mL

Storage Buffer: Deionized water with 0.02% sodium azide and 0.01% NP-4O
Course of
A dilution of 1-2 drops to 1mL will current an sufficient number of particles for motion cytometry use.

 

Notes: Shake vigorously or vortex briefly sooner than use. Inclusion of a small amount of detergent inside the diluent will help enhance the number of singlets. Diluted particles could also be saved inside the fridge for future use.

QPCR Kit QRT-PCR Mastermix RNA - EACH

MOL6356 EACH
EUR 345.6

PanProbes™ Universal qPCR MasterMix w Low ROX

QPD01-L100 100 rxns
EUR 20

TaqProbe 5X qPCR MasterMix-Low ROX

MasterMix-5PL 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 255.6

TaqProbe 2X qPCR MasterMix-Low ROX

MasterMix-PL 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

Bio-Star Mastermix (2X) Low-ROX

P150 100 rcs (2,5 ml)
EUR 122.4

KiloGreen 2X qPCR MasterMix-Low ROX

MasterMix-KL 4 x 1.25 ml - 500 reactions (20 ul)
EUR 168

BrightGreen 5X qPCR MasterMix-Low ROX

MasterMix-5L 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 255.6

BrightGreen 2X qPCR MasterMix-Low ROX

MasterMix-LR 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

BrightGreen 2X qPCR MasterMix-Low ROX

MasterMix-LR-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 451.2

BrightGreen miRNA qPCR MasterMix-Low ROX

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Multiplex PCR Mastermix (2X) No-ROX

M100-1000 1000 rcs (12,5 ml)
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Multiplex PCR Mastermix (2X) No-ROX

M100-200 200 rcs (2,5 ml)
EUR 329

Multiplex PCR Mastermix (2X) High-ROX

M120-1000 1000 rcs (12,5 ml) Ask for price

Multiplex PCR Mastermix (2X) High-ROX

M120-200 200 rcs (2,5 ml) Ask for price

Green-2-Go 1-Step qRT-PCR Mastermix-iCycler

QPCR011-iC 100Rxn
EUR 287.08

PanGreen™ Universal SYBR® Green Mastermix w Low ROX

QSD01-L100 100 rxns
EUR 20

BrightGreen Express 2X qPCR MasterMix-Low ROX

MasterMix-EL 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

PanProbes™ Universal qPCR MasterMix UNG w Low ROX

QPD02-L100 100 rxns
EUR 22

Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn)

QPCR002-L 4X1.25ml
EUR 303.25

PanGreen™ Universal SYBR Green Mastermix UNG w Low ROX

QSD02-L100 100 rxns
EUR 22

Bio-Star Mastermix with SYBR (2X) Low-ROX

Y250 100 rcs (2,5 ml)
EUR 122.4

Green-2-Go 1-Step qRT-PCR Mastermix-No Dye (100X20ul Rxn)

QPCR007-NODYE 100Rxn
EUR 287.08

Real-Time PCR Mastermix 2x, Low Level of Rox , 100RXNs, optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye

ACT-RT400LR each
EUR 261.6

Taq 2X PCR MasterMix

G888 400 rxn (10.0 ml)
EUR 60
Description: Taq 2X PCR MasterMix is a ready-to-use MasterMix containing Taq DNA Polymerase (Cat. No. G009) in a uniquely-formulated buffer with gel loading dye. Taq has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with Taq can be used with TA cloning vectors.

Kodaq 2X PCR MasterMix

G497 5.0 ml (200 Rxns)
EUR 189.6

BlasTaq 2X PCR MasterMix

E36G895 800 rxn (10.0 ml)
EUR 342.86

BlasTaq 2X PCR MasterMix

G895 800 rxn (10.0 ml)
EUR 275
Description: BlasTaq™ 2X PCR MasterMix is a ready-to-use MasterMix containing abm’s BlasTaq™ DNA Polymerase in a uniquely-formulated buffer with gel loading dye. This strategicallyengineered, next generation Taq Polymerase provides rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaq™ can be used with TA cloning vectors.

BlasTaq 2X PCR MasterMix

P895-1 100 rxn (1.25 ml)
EUR 60
Description: BlasTaq™ 2X PCR MasterMix is a ready-to-use MasterMix containing abm’s BlasTaq™ DNA Polymerase in a uniquely-formulated buffer with gel loading dye. This strategicallyengineered, next generation Taq Polymerase provides rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaq™ can be used with TA cloning vectors.

2X PCR Taq MasterMix

G900 25.0 ml (1000 Rxns)
EUR 246

2X PCR Taq MasterMix

G013 5.0 ml (200 Rxns)
EUR 104.4

Green-2-Go 1-Step TaqProbe qRT-PCR Mastermix-no Dye (100X20ul Rxn)

QPCR008-NODYE 100Rxn
EUR 341.88

Robert Brown