
Ultra Rainbow Fluorescent Particles SPHERO
Motion Cytometry Alignment Particles
Spherotech offers every kind of particles for determining if a motion cytometer is appropriately aligned and has a transparent motion cell with no fluidic blockage. Using SPHERO™ Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions could also be measured to seek out out the alignment and efficiency of the motion cytometer. Additiona information could also be found at STN-8: Calibration and Effectivity Monitoring of Motion Cytometer Using SPHERO Calibration Particles.
Spherotech Cat. No. URFP-30-2 Instructions for use:
A. Preparation of Particles
1. Vortex the particles vigorously
2. Add 2 to 4 drops of particles to 1mL of sheath fluid or DI water. The inclusion of a small amount of detergent (~0.01%) inside the dilution buffer will enhance the proportion of the singlet inhabitants.
B. Every day Alignment
To search out out the optical alignment of the system perform the following:
1. Set a keep gate for the singlet inhabitants on the FSC vs SSC histogram to exclude aggregates
2. Modify the Purchase and Extreme voltage so that the suggest channel number of the peak is in a predetermined place on each histogram of curiosity. The histograms on the sooner internet web page will be utilized as a info.
3. Accumulate 5000 events contained within the gate
4. Report the % CV and Extreme Voltage for all fluorescence channels of curiosity.
5. Use a computer program resembling Excel to generate the Levy Jennings graphs.
NOTE: If the values on any parameter exceed these of the day-to-day frequent or preset values, which can be determined by not lower than one months worth of knowledge, additional calibration or alignment procedures have to be carried out in response to the instrument operation handbook.

|
Description
Extraordinarily Rainbow Fluorescent Particles (by Spherotech™) are internally labeled with quite a lot of fluorophores. They exhibit full spectrum fluorescence, along with enhanced UV and Far Pink fluorescence. The mixture of fluorophores permits Extraordinarily Rainbow particles to be excited at any wavelength from 365 to 650nm. These particles will be present in two diameters, ~3.8µm and ~10.2µm.
Traits
Suggest Diameter: ~3.8µm (3.61-3.99µm) or ~10.2µm (8.1 – 12.0µm); see your Certificates of Analysis for exact measurement.
Focus: ~2 x 106particles/mL
Storage Buffer: Deionized water with 0.02% sodium azide and 0.01% NP-4O
Course of
A dilution of 1-2 drops to 1mL will current an sufficient number of particles for motion cytometry use.
Notes: Shake vigorously or vortex briefly sooner than use. Inclusion of a small amount of detergent inside the diluent will help enhance the number of singlets. Diluted particles could also be saved inside the fridge for future use.
![]() BrightGreen miRNA qPCR MasterMix-Low ROX |
|||
MasterMix-mL | ABM | 4 x 1.25 ml for 500 reactions | EUR 181.2 |
![]() TaqProbe 2X qPCR MasterMix-Low ROX |
|||
MasterMix-PL | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
![]() BrightGreen Express 2X qPCR MasterMix-Low ROX |
|||
MasterMix-EL | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
![]() BrightGreen 2X qPCR MasterMix-Low ROX |
|||
MasterMix-LR-XL | ABM | 16 x 1.25 ml for 2000 reactions (20 ul) | EUR 451.2 |
![]() Green-2-Go 1-Step qRT-PCR Mastermix-Low Rox |
|||
QPCR012-LR | Bio Basic | 100Rxn | EUR 287.08 |
![]() TaqProbe 5X qPCR MasterMix-ROX |
|||
MasterMix-5P | ABM | 4 x 1.0 ml for 1000 reactions (20 ul) | EUR 255.6 |
![]() BrightGreen 5X qPCR MasterMix-ROX |
|||
MasterMix-5R | ABM | 4 x 1.0 ml for 1000 reactions (20 ul) | EUR 255.6 |
![]() KiloGreen 2X qPCR MasterMix-ROX |
|||
MasterMix-KR | ABM | 4 x 1.25 ml - 500 reactions (20 ul) | EUR 168 |
![]() BrightGreen miRNA qPCR MasterMix-ROX |
|||
MasterMix-mR | ABM | 4 x 1.25 ml for 500 reactions | EUR 181.2 |
![]() TaqProbe 2X qPCR MasterMix-ROX |
|||
MasterMix-P | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
![]() BrightGreen 2X qPCR MasterMix-ROX |
|||
MasterMix-R | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
![]() One-Step BrightGreen qRT-PCR-Low ROX |
|||
G471-LR | ABM | 100 rxn (20 ul/rxn) | EUR 189.6 |
![]() One-Step TaqProbe qRT-PCR-Low ROX |
|||
G493-PL | ABM | 100 rxn (20 ul/rxn) | EUR 189.6 |
![]() BrightGreen Express 2X qPCR MasterMix-ROX |
|||
MasterMix-ER | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
![]() BrightGreen 2X qPCR MasterMix-ROX |
|||
MasterMix-R-XL | ABM | 16 x 1.25 ml for 2000 reactions (20 ul) | EUR 451.2 |
![]() ExCellenCT One-Step BrightGreen qRT-PCR-Low ROX |
|||
G917-LR | ABM | 25 Preps 100 x 20 ul reactions | EUR 212.4 |
![]() ExCellenCT One-Step TaqProbe qRT-PCR-Low ROX |
|||
G918-PL | ABM | 25 Preps 100 x 20 ul reactions | EUR 212.4 |
![]() Jade? QRT Kit-Low ROX |
|||
M1177-100 | Biovision | each | EUR 444 |
![]() Taqman QRT Kit-Low ROX |
|||
M1185-100 | Biovision | each | EUR 444 |
![]() Green-2-Go 1-Step qRT-PCR Mastermix-Rox (100X20ul Rxn) |
|||
QPCR010-R | Bio Basic | 100Rxn | EUR 287.08 |
![]() QPCR Kit QRT-PCR Mastermix RNA |
|||
MOL6356 | Scientific Laboratory Supplies | EACH | EUR 257.64 |
![]() Org Jade? QRT Kit-Low ROX |
|||
M1181-100 | Biovision | each | EUR 529.2 |
![]() Org Taqman QRT Kit-Low ROX |
|||
M1189-100 | Biovision | each | EUR 529.2 |
![]() Bio-Star Mastermix (2X) Low-ROX |
|||
P150 | GeneOn | 100 rcs (2,5 ml) | EUR 122.4 |
![]() One-Step BrightGreen qRT-PCR-ROX |
|||
G471-R | ABM | 100 rxn (20 ul/rxn) | EUR 189.6 |
![]() One-Step TaqProbe qRT-PCR-ROX |
|||
G493-P | ABM | 100 rxn (20 ul/rxn) | EUR 189.6 |
![]() ExCellenCT One-Step BrightGreen qRT-PCR-ROX |
|||
G917-R | ABM | 25 Preps 100 x 20 ul reactions | EUR 212.4 |
![]() ExCellenCT One-Step TaqProbe qRT-PCR-ROX |
|||
G918-P | ABM | 25 Preps 100 x 20 ul reactions | EUR 212.4 |
![]() Bio-Star Mastermix with SYBR (2X) Low-ROX |
|||
Y250 | GeneOn | 100 rcs (2,5 ml) | EUR 122.4 |
![]() Jade? QRT Kit-ROX |
|||
M1178-100 | Biovision | each | EUR 444 |
![]() Taqman QRT Kit-ROX |
|||
M1186-100 | Biovision | each | EUR 444 |