RNase R that converts 1 µg of poly(A)

Biovision rnase-r

SAMPLE PROCESSING GUIDELINES AND TROUBLESHOOTING:

• For digestion of full RNA, longer incubations of 2-Three hours are generally required.
• If degradation is inefficient, use a barely elevated incubation temperature (40-45°C) and complement additional enzyme partway (e.g. 0.5 μl after 1 hour) by way of the method. The higher temperature is particularly useful for degrading extraordinarily structured linear RNAs, equal to rRNAs. Do not exceed 45°C or incubate over 3
hours, as this may end in non-enzymatic RNA degradation.

• RNase R shows low train on tRNA, rRNA and totally different extraordinarily structured RNAs, for which the three’ end is double stranded with a short 3’ overhang. These RNA species can stall the enzyme and finish in drastically lowered train. If inefficient degradation is seen, it is endorsed to each upscale the digestion, use additional RNase R,
or take away rRNA from full RNA extracts earlier to digestion.

• Keep in mind that spherical RNAs symbolize a small proportion of full RNA (generally 0.1%-0.01%), because of this reality RNase R treatment will most actually finish in low ranges of RNA (picogram-range), presumably undetectable by most methods. For that purpose, a starting amount of not lower than 10 µg of full RNA is useful for a lot of downstream features.

• Whereas the enzyme could also be heat inactivated the method should not be useful since extreme heat can lead to RNA hurt. Phenol-chloroform precipitation could be utilized instead. For NGS, robust half reversible immobilization (SPRI) bead cleanup is useful.

• Magnesium at concentrations of 0.1-1.Zero mM is required for optimum train. If ETA is
present, compensate by together with MgCl2 to 1.Zero Mm final focus.

DESCRIPTION:

RNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species. This enzyme does not digest spherical, lariat, or double stranded RNA with temporary 3’ overhangs (decrease than sevennucleotides). As such, this enzyme is ideally suited to the study of lariat RNA produced by typical splicing, along with circRNAs which come up by way of back-splicing.

APPLICATIONS:
• Enriching circRNAs in natural samples
• Identification of intronic lariat sequences
• Identification of exonic circRNAs
• Studying varied splicing
• Manufacturing of artificial spherical RNAs

EZYME UNIT DEFINITION: One unit is printed as the amount of RNase R that converts 1
µg of poly(A) into acid soluble nucleotides in 10 minutes at 37°C. ENZYME STORAGE BUFFER: 50 mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol 10X RNase R REACTION BUFFER COMPONENTS: 200 mM Tris-HCl, 1 M KCl, 1 mM, MgCl2, pH 7.5.

STORAGE CONDITIONS: Retailer at -20°C.

Stay away from repeated freeze-thaw cycles of all components to retain most effectivity. All components
are regular for one 12 months from the date of transport when saved and handled appropriately.

 

Cat # +DimensionM1228-500
Dimension500 U
HighlightsRNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species.
Storage Conditions-20°C
Supply ConditionsGel Pack
USAGEFor Evaluation Use Solely! Not For Use in Individuals.
Biovision rnase-r
Biovision rnase-r

 

 

RELATED PRODUCTS:

• RNase A (Cat# M1227-25)
• RNaseOFF ribonuclease Inhibitor (Cat# M1238-4000)
• DNase, E.coli DNA Ligase (Cat# M1217-100)
• Hyperlink-FAST 5 Minutes DNA Ligation Package deal (Cat# Okay902-50)
• New T4 DNA Ligase (Cat# M1247-200)
• T4 DNA Ligase (5 u/μl) (Cat# 9101-250)
• T4 RNA Ligase 1 (ssRNA Ligase) (Cat# M1218-100)
• T4 RNA Ligase 2 (dsRNA Ligase) (Cat# M1219-100)

 

RNase H (RNase-free)

K1093-500 500U
EUR 24
Description: RNase H

RNase H (RNase-free)

K1093-5000 5000U
EUR 120
Description: RNase H

RNAse

E16FXP0211R-200 200 μl
EUR 13.33
Description: Available in various conjugation types.

RNAse

E16FXP0211R-400 400 μl
EUR 20
Description: Available in various conjugation types.

RNAse

E16FXP0211R-800 800 μl
EUR 26.67
Description: Available in various conjugation types.

RNase A

abx098139-1ml 1 ml
EUR 260.4

PI / RNAse

abx290029-200tests 200 tests
EUR 326.4

RNase A

IB47163 50ul
EUR 20.72
Description: (FOR HI-SPEED PLASMID) [FR}

RNase A

G117 25 mg
EUR 55
Description: RNase A is pancreatic bovine endoribonuclease that cleaves single stranded RNA by hydrolysis at cytosine and uracil residues. RNase A is very useful for the removal of RNA from plasmid DNA preparations.

RNase A

M1227-25 each
EUR 294

RNase A

RB0473 500mg
EUR 219.73

RNase H

RK20535 250U
EUR 88

RNase A

RN103-001 300mg
EUR 3138

RNase A

MB087-100MG 1 unit
EUR 34.02
Description: RNase A

RNase A

MB087-1G 1 unit
EUR 284.22
Description: RNase A

RNase A

MB087-500MG 1 unit
EUR 153.8
Description: RNase A

RNase A

RM7705-100MG 1 unit
EUR 136.11
Description: RNase A

RNase A

RM7705-1G 1 unit
EUR 1102.26
Description: RNase A

RNase A

RM7705-500MG 1 unit
EUR 612.22
Description: RNase A

RNase A

RM1555-100MG 1 unit
EUR 118.89
Description: RNase A

RNase A

RM1555-500MG 1 unit
EUR 537.26
Description: RNase A

RNase A

abx098139-100l 100 µl
EUR 250

RNase A

abx098139-200l 200 µl Ask for price

Rnase (90–105)

5-01885 4 x 1mg Ask for price

RNase Quiet

09147-14 475ML
EUR 26.6

Rnase A, 250mg

R9001-250mg each
EUR 337.2

RNase Inhibitor

30260-54 2500UNITS
EUR 83.3

RNase Inhibitor

30260-96 12500UNITS
EUR 315

RNase Inhibitor

EQ010 1000U
EUR 33.5

RNase Inhibitor

abx460013-1096tests 10 × 96 tests Ask for price

RNase Inhibitor

abx460013-596tests 5 × 96 tests Ask for price