Rapid and efficient generation of antigen-specific isogenic T cells from cryopreserved blood samples

CRISPR/Cas9-mediated gene modifying has been leveraged for the modification of human and mouse T cells. Nevertheless, restricted expertise is on the market on the applying of CRISPR/Cas9 electroporation in cryopreserved T cells collected throughout scientific trials. To deal with this, we aimed to optimize a CRISPR/Cas9-mediated gene modifying protocol appropriate with peripheral blood mononuclear cells…

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