QPCR Retrovirus Titration kits require solely 4 hours

qPCR and One-Step RT MasterMixes

Virus Titration Kits

Our Titration Kits current a fast and straightforward approach for titrating your virus. The kits make use of each a quick RNA or DNA extraction step, and resolve viral RNA/DNA titer using qRT-PCR.

Advantages of abm’s titration kits:

  • No RNA purification — saves time and eliminates inaccuracy.
  • Ready-to-use reagent mix — reduces variability.
  • qRT-PCR in a single step — additional delicate and proper than completely different methods.
  • No NTC amplification — Distinctive primer design completely eliminates non-specific amplification in NTCs.

We moreover provide On the spot Lentivirus Titration Taking part in playing cards, which allow quick detection of lentiviral capsule proteins as a marker to substantiate the presence of lentivirus. Utilizing the lateral flow into immunoassay approach, viral titer yields are assessed instantly inside 10 minutes using our three step protocol.

he Retro-X qRT-PCR Titration Tools provides a fast and straightforward approach for measuring retrovirus titer. The kits use a quick RNA purification step sooner than quantifying the number of retroviral genome copies using qRT-PCR and TB Inexperienced utilized sciences.

Description

abm’s qPCR Retrovirus Titer Tools is a one-step assay by qPCR which does not require additional lysis or reverse transcription (RT) steps, and might be completed beneath one hour. Designed to ship extreme sensitivity and specificity, the tools ensures minimal non-specific background and better complete effectivity as compared with comparable kits within the market. ROX reference dye is obtainable separate from the MasterMix, making this tools universally appropriate with most qPCR gadgets.

qPCR and One-Step RT MasterMixes
qPCR and One-Step RT MasterMixes

 

PRODUCT COMPONENTQUANTITY
BlasTaq 2X qPCR Titer MasterMix1.25 ml
Primer Mix100 rxn (200 µl)
Commonplace Administration DNA50 µl
ROX Reference Dye15 µl
Nuclease-Free H2O2 x 1.zero ml

 

SKUG949
Unit quantity100 rxn
Storage State of affairsRetailer at -20°C

 

Quick willpower of retroviral titer

Whereas standard titration methods require as a lot as 10 days to complete, these titration kits require solely 4 hours and work with all MMLV-based retroviral vectors (not MSCV). Using qRT-PCR dramatically shortens the time interval between viral harvest and target-cell an an infection, allowing you to hold out every on the equivalent day.

Receive fixed retroviral transductions

A critical good thing about retroviral gene-delivery strategies is the facility to manage the extent of gene expression by adjusting the multiplicity of an an infection (MOI) to dictate the number of viral genomes that enter your objective cells. These titration kits allow you to make a quick correlation between the retroviral copy amount and retroviral infectivity in your cell type and in the long run allow you to administration the expression stage between experiments.

The best way it really works

The qRT-PCR course of entails subjecting serial dilutions of your sample and a administration RNA of acknowledged copy amount (provided) to one-step qRT-PCR. The retroviral copy amount contained in your sample can then be determined by evaluating its Ct value to the standard curve.

RNA purification and DNase I remedy steps are essential to the protocol so even viruses packaged using transient transfections, which always embrace residual plasmid DNA, might be exactly quantified using this tools.

 

Rat Serum IgM detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80155-M 1 kit
EUR 781.2

Mouse Serum IgM detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-M 1 kit
EUR 781.2

Mouse Serum IgG3 detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-G3 1 kit
EUR 781.2

Lentivirus Titration XpressCard

ATG-LT-01-10 10 tests
EUR 195

Lentivirus Titration XpressCard

ATG-LT-01-20 20 tests
EUR 335

Lentivirus Titration XpressCard

ATG-LT-01-50 50 tests
EUR 715

Lentivirus Titration XpressCard

ATG-LT-01-6 6 tests
EUR 136

Mouse Primary Antibody (IgA) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-A 1 kit
EUR 781.2

Mouse Primary Antibody (IgE) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-E 1 kit
EUR 781.2

Rat Primary Antibody (IgA) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80155-A 1 kit
EUR 781.2

Rat Primary Antibody (IgE) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80155-E 1 kit
EUR 781.2

Instant Lentivirus Titration Card

G614 10 Tests
EUR 235

Instant Lentivirus Titration Card

G615 20 Tests
EUR 405

Mouse Primary Antibody (IgG1) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-G1 1 kit
EUR 781.2

Mouse Primary Antibody (IgG2a) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-G2a 1 kit
EUR 781.2

Mouse Primary Antibody (IgG2b) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-G2b 1 kit
EUR 781.2

Mouse Primary Antibody (A+G+M) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150 1 Kit
EUR 781.2

Rat Primary Antibody (A+G+M) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80155 1 Kit
EUR 781.2