QPCR Retrovirus Titration kits require solely 4 hours

qPCR and One-Step RT MasterMixes

Virus Titration Kits

Our Titration Kits current a fast and straightforward approach for titrating your virus. The kits make use of each a quick RNA or DNA extraction step, and resolve viral RNA/DNA titer using qRT-PCR.

Advantages of abm’s titration kits:

  • No RNA purification — saves time and eliminates inaccuracy.
  • Ready-to-use reagent mix — reduces variability.
  • qRT-PCR in a single step — additional delicate and proper than completely different methods.
  • No NTC amplification — Distinctive primer design completely eliminates non-specific amplification in NTCs.

We moreover provide On the spot Lentivirus Titration Taking part in playing cards, which allow quick detection of lentiviral capsule proteins as a marker to substantiate the presence of lentivirus. Utilizing the lateral flow into immunoassay approach, viral titer yields are assessed instantly inside 10 minutes using our three step protocol.

he Retro-X qRT-PCR Titration Tools provides a fast and straightforward approach for measuring retrovirus titer. The kits use a quick RNA purification step sooner than quantifying the number of retroviral genome copies using qRT-PCR and TB Inexperienced utilized sciences.

Description

abm’s qPCR Retrovirus Titer Tools is a one-step assay by qPCR which does not require additional lysis or reverse transcription (RT) steps, and might be completed beneath one hour. Designed to ship extreme sensitivity and specificity, the tools ensures minimal non-specific background and better complete effectivity as compared with comparable kits within the market. ROX reference dye is obtainable separate from the MasterMix, making this tools universally appropriate with most qPCR gadgets.

qPCR and One-Step RT MasterMixes
qPCR and One-Step RT MasterMixes

 

PRODUCT COMPONENTQUANTITY
BlasTaq 2X qPCR Titer MasterMix1.25 ml
Primer Mix100 rxn (200 µl)
Commonplace Administration DNA50 µl
ROX Reference Dye15 µl
Nuclease-Free H2O2 x 1.zero ml

 

SKUG949
Unit quantity100 rxn
Storage State of affairsRetailer at -20°C

 

Quick willpower of retroviral titer

Whereas standard titration methods require as a lot as 10 days to complete, these titration kits require solely 4 hours and work with all MMLV-based retroviral vectors (not MSCV). Using qRT-PCR dramatically shortens the time interval between viral harvest and target-cell an an infection, allowing you to hold out every on the equivalent day.

Receive fixed retroviral transductions

A critical good thing about retroviral gene-delivery strategies is the facility to manage the extent of gene expression by adjusting the multiplicity of an an infection (MOI) to dictate the number of viral genomes that enter your objective cells. These titration kits allow you to make a quick correlation between the retroviral copy amount and retroviral infectivity in your cell type and in the long run allow you to administration the expression stage between experiments.

The best way it really works

The qRT-PCR course of entails subjecting serial dilutions of your sample and a administration RNA of acknowledged copy amount (provided) to one-step qRT-PCR. The retroviral copy amount contained in your sample can then be determined by evaluating its Ct value to the standard curve.

RNA purification and DNase I remedy steps are essential to the protocol so even viruses packaged using transient transfections, which always embrace residual plasmid DNA, might be exactly quantified using this tools.

 

Retrovirus Maxi Purification Kit

K1308-2 each
EUR 639.6

Retrovirus Maxi Purification Kit

K1308-4 each
EUR 1032

Retrovirus Booster 50µl

ABP-RVC-RBST05 50µl Ask for price

Retrovirus Booster 200µl

ABP-RVC-RBST20 200µl Ask for price

Retrovirus Booster 400µl

ABP-RVC-RBST40 400µl Ask for price

Retrovirus iPSC Reprogramming Kit

RF101 1 Kit
EUR 782.43

Retrovirus-related Gag

E8R1706-5 100ul
EUR 275
Description: Available in various conjugation types.

Retrovirus Stabilizer 50µl

ABP-RVC-RSTB05 50µl Ask for price

Retrovirus Stabilizer 200µl

ABP-RVC-RSTB20 200µl Ask for price

Retrovirus Stabilizer 400µl

ABP-RVC-RSTB40 400µl Ask for price

iPS Cell Line (Retrovirus)

ASE-9101 1 vial of ^2.5X10^5 cells
EUR 516.84
Description: 6 month

M13 Phage Titration ELISA Kit

55R-PRPHAGE 96 tests
EUR 831.6
Description: Enzyme Immunoassay for the Quantitative Determination of purified M13 Bacteriophage Particles

AAV5 Titration ELISA Kit (Fast)

AAV-A005H 96tests
EUR 706.2
Description: AAV5 Titration ELISA Kit(Fast) is based on ELISA sandwich method and designed to detect and quantitative determine of AAV5 capsid titration in AAV gene therapy product preparation processing. It contains AAV5 empty capsids and a pair of antibodies against AAV5. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of AAV5 capsid titration.

QuickTiter Retrovirus Quantitation Kit

VPK-120 20 assays
EUR 853.2
Description: Our QuickTiter Retrovirus Quantitation Kit provides a quick method for determining retroviral titer. This assay measures the viral nucleic acid content of your retrovirus, and may be performed either before or after purification of your virus.

Monkey Simian RetroVirus,SRV ELISA Kit

YX-SL0039Mk - Ask for price

Retrovirus Precipitation Solution

VC200 100 ml
EUR 194.04

Retrovirus Precipitation Solution

VC225 250 ml
EUR 432.6

Retrovirus Precipitation Solution

VC250 500 ml
EUR 814.8