RNase R that converts 1 µg of poly(A)
SAMPLE PROCESSING GUIDELINES AND TROUBLESHOOTING:
• For digestion of full RNA, longer incubations of 2-Three hours are generally required.
• If degradation is inefficient, use a barely elevated incubation temperature (40-45°C) and complement additional enzyme partway (e.g. 0.5 μl after 1 hour) by way of the method. The higher temperature is particularly useful for degrading extraordinarily structured linear RNAs, equal to rRNAs. Do not exceed 45°C or incubate over 3
hours, as this may end in non-enzymatic RNA degradation.
• RNase R shows low train on tRNA, rRNA and totally different extraordinarily structured RNAs, for which the three’ end is double stranded with a short 3’ overhang. These RNA species can stall the enzyme and finish in drastically lowered train. If inefficient degradation is seen, it is endorsed to each upscale the digestion, use additional RNase R,
or take away rRNA from full RNA extracts earlier to digestion.
• Keep in mind that spherical RNAs symbolize a small proportion of full RNA (generally 0.1%-0.01%), because of this reality RNase R treatment will most actually finish in low ranges of RNA (picogram-range), presumably undetectable by most methods. For that purpose, a starting amount of not lower than 10 µg of full RNA is useful for a lot of downstream features.
• Whereas the enzyme could also be heat inactivated the method should not be useful since extreme heat can lead to RNA hurt. Phenol-chloroform precipitation could be utilized instead. For NGS, robust half reversible immobilization (SPRI) bead cleanup is useful.
• Magnesium at concentrations of 0.1-1.Zero mM is required for optimum train. If ETA is
present, compensate by together with MgCl2 to 1.Zero Mm final focus.
DESCRIPTION:
RNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species. This enzyme does not digest spherical, lariat, or double stranded RNA with temporary 3’ overhangs (decrease than sevennucleotides). As such, this enzyme is ideally suited to the study of lariat RNA produced by typical splicing, along with circRNAs which come up by way of back-splicing.
APPLICATIONS:
• Enriching circRNAs in natural samples
• Identification of intronic lariat sequences
• Identification of exonic circRNAs
• Studying varied splicing
• Manufacturing of artificial spherical RNAs
EZYME UNIT DEFINITION: One unit is printed as the amount of RNase R that converts 1
µg of poly(A) into acid soluble nucleotides in 10 minutes at 37°C. ENZYME STORAGE BUFFER: 50 mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol 10X RNase R REACTION BUFFER COMPONENTS: 200 mM Tris-HCl, 1 M KCl, 1 mM, MgCl2, pH 7.5.
STORAGE CONDITIONS: Retailer at -20°C.
Stay away from repeated freeze-thaw cycles of all components to retain most effectivity. All components
are regular for one 12 months from the date of transport when saved and handled appropriately.
| Cat # +Dimension | M1228-500 |
|---|---|
| Dimension | 500 U |
| Highlights | RNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species. |
| Storage Conditions | -20°C |
| Supply Conditions | Gel Pack |
| USAGE | For Evaluation Use Solely! Not For Use in Individuals. |
RELATED PRODUCTS:
• RNase A (Cat# M1227-25)
• RNaseOFF ribonuclease Inhibitor (Cat# M1238-4000)
• DNase, E.coli DNA Ligase (Cat# M1217-100)
• Hyperlink-FAST 5 Minutes DNA Ligation Package deal (Cat# Okay902-50)
• New T4 DNA Ligase (Cat# M1247-200)
• T4 DNA Ligase (5 u/μl) (Cat# 9101-250)
• T4 RNA Ligase 1 (ssRNA Ligase) (Cat# M1218-100)
• T4 RNA Ligase 2 (dsRNA Ligase) (Cat# M1219-100)
, Biotinylated) Histone H3 Peptide (residues 21-44), Biotinylated |
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| R-1006 | EpiGentek |
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, Biotinylated) Histone H4 Peptide (residues 1-21), Biotinylated |
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| R-1007 | EpiGentek |
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 Histone Acetyl H4ac Peptide, Biotinylated |
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| R-1008 | EpiGentek |
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 Histone Acetyl H3K9ac Peptide |
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| R-1009 | EpiGentek |
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 Histone Acetyl H3K9ac Peptide, Biotinylated |
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| R-1010 | EpiGentek |
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 Histone Acetyl H3K14ac Peptide |
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| R-1011 | EpiGentek |
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 Histone Acetyl H3K14ac Peptide, Biotinylated |
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| R-1012 | EpiGentek |
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 Histone Acetyl H4K5ac Peptide |
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| R-1014 | EpiGentek |
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 Histone Acetyl H4K8ac Peptide |
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| R-1015 | EpiGentek |
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 Histone Acetyl H4K12ac Peptide |
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| R-1016 | EpiGentek |
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 Histone Acetyl H4K16ac Peptide |
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| R-1017 | EpiGentek |
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 Histone H3K4me1 Monomethyl Peptide |
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| R-1018 | EpiGentek |
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 Histone H3K4me1 Monomethyl Peptide, Biotinylated |
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| R-1019 | EpiGentek |
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 Histone H3K4me2 Dimethyl Peptide |
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| R-1020 | EpiGentek |
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 Histone H3K4me2 Dimethyl Peptide, Biotinylated |
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| R-1021 | EpiGentek |
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 Histone H3K4me3 Trimethyl Peptide |
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| R-1022 | EpiGentek |
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 Histone H3K4me3 Trimethyl Peptide, Biotinylated |
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| R-1023 | EpiGentek |
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 Histone H3K9me1 Monomethyl Peptide |
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| R-1024 | EpiGentek |
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 Histone H3K9me1 Monomethyl Peptide, Biotinylated |
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| R-1025 | EpiGentek |
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 Histone H3K9me2 Dimethyl Peptide |
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| R-1026 | EpiGentek |
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 Histone H3K9me2 Dimethyl Peptide, Biotinylated |
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| R-1027 | EpiGentek |
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 Histone H3K9me3 Trimethyl Peptide |
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| R-1028 | EpiGentek |
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 Histone H3K9me3 Trimethyl Peptide, Biotinylated |
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| R-1029 | EpiGentek |
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 Histone H3K27me1 Monomethyl Peptide |
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| R-1030 | EpiGentek |
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 Histone H3K27me1 Monomethyl Peptide, Biotinylated |
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| R-1031 | EpiGentek |
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 Histone H3K27me2 Dimethyl Peptide |
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| R-1032 | EpiGentek |
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 Histone H3K27me2 Dimethyl Peptide, Biotinylated |
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| R-1033 | EpiGentek |
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 Histone H3K27me3 Trimethyl Peptide |
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| R-1034 | EpiGentek |
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 Histone H3K27me3 Trimethyl Peptide, Biotinylated |
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| R-1035 | EpiGentek |
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 Histone H3K36me1 Monomethyl Peptide |
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| R-1036 | EpiGentek |
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 Histone H3K36me1 Monomethyl Peptide, Biotinylated |
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| R-1037 | EpiGentek |
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