Cellexus Limited Cell Culture Biosystems

CellMaker disposable bioreactors for Normal human Cells

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A Kinetic Pump Integrated Microfluidic Plate (KIM-Plate) with High Usability for Cell Culture-Based Multiorgan Microphysiological Systems

Microphysiological programs (MPSs), together with organ-on-a-chip (OoC), have attracted consideration as a novel technique for estimating the consequences and uncomfortable side effects of medication in drug discovery. To breed the dynamic in vivo atmosphere, earlier MPSs have been linked to pump programs to perfuse tradition medium. Due to this fact, most MPSs usually are not user-friendly and have poor throughput. We aimed to develop a kinetic pump built-in microfluidic plate (KIM-Plate) by making use of the stirrer-based micropump to an open entry tradition plate to enhance the usability of MPSs.
The KIM-Plate integrates six multiorgan MPS (MO-MPS) items and meets the ANSI/SBS microplate requirements. We evaluated the perfusion perform of the kinetic pump and located that the KIM-Plate had enough agitation impact. Coculture experiments with PXB cells and hiPS intestinal cells confirmed that the TEER of hiPS intestinal cells and gene expression ranges associated to the metabolism of PXB cells have been elevated. Therefore, the KIM-Plate is an modern instrument for the straightforward coculture of extremely conditioned cells that’s anticipated to facilitate cell-based assays within the fields of drug discovery and biology due to its usability and excessive throughput nature.
Iron is essential to the regulation of the host innate immune system and the result of many infections. Hepatitis C virus (HCV), one of many main viral human pathogens that is dependent upon iron to finish its life cycle, is extremely expert in evading the immune system.
  • This examine presents the development and validation of a physiologically related triple-cell co-culture mannequin that was used to research the enter of iron in HCV an infection and the interaction between HCV, iron, and determinants of host innate immunity.
  • We recorded the expression patterns of key proteins of iron homeostasis concerned in iron import, export and storage and examined their relation to the iron regulatory hormone hepcidin in hepatocytes, enterocytes and macrophages within the presence and absence of HCV.
  • We then assessed the transcriptional profiles of pro-inflammatory cytokines Interleukin-6 (IL-6) and interleukin-15 (IL-15) and anti inflammatory interleukin-10 (IL-10) below regular or iron-depleted circumstances and decided how these have been affected by an infection.
  • Our knowledge counsel the presence of a hyperlink between iron homeostasis and innate immunity unfolding amongst liver, gut, and macrophages, which may take part within the deregulation of innate immune responses noticed in early HCV an infection.
  • Coupled with iron-assisted enhanced viral propagation, such a mechanism could also be vital for the institution of viral persistence and the following continual liver illness.

Bacterial CellCultures in a Lab-on-a-Disc: a Simple and Versatile System for Quantification of Antibiotic Treatment Efficacy

Pathogenic bacterial biofilms might be life threatening, tremendously decrease affected individual’s prime quality of life and are a substantial burden on the healthcare system. Current methods for evaluation of antibacterial therapies in clinics and in vitro strategies utilized in drug development and screening each do not facilitate biofilm formation or are cumbersome to perform, need large reagent volumes and are costly, limiting their usability.

To deal with these factors, this work presents the occasion of a sturdy in vitro cell custom platform appropriate with confocal microscopy.

The platform fashioned as a compact disc, facilitates long term bacterial custom with out exterior pumps and tubing and might be operated for numerous days with out additional liquid coping with. For example, Pseudomonas aeruginosa biofilm is grown from single cells and it is confirmed that:

1) the platform delivers reproducible and reliable outcomes; 2) progress depends on motion cost and progress medium composition; and three) effi-cacy of antibiotic treatment will rely upon the formed biofilm. This platform permits biofilm progress, quantification and treatment as in a standard motion setup, whereas decreasing the equipment barrier of lab-on-chip strategies. It offers an easy-to-use, moderately priced chance for end prospects working with cell culturing in relation to e.g. diagnostics and drug screening.

Clear Microcrystalline Cellulose/Polyvinyl Alcohol Paper as New Platform for 3D cell tradition

Multilayered and stacked cellulose paper has emerged as a promising platform for constructing of three-dimensional (3D) cell custom attributable to its low value, good biocompatibility and extreme porosity. Nonetheless, its poor delicate transmission makes it tough to immediately and clearly monitor cell behaviors (e.g., progress and proliferation) on the paper-based platform using optical microscope.

On this work, we developed a transparent microcrystalline cellulose/polyvinyl alcohol (MCC/PVA) paper with irregular pores through dissolution and regeneration of microcrystalline nanocellulose (MCC), addition of porogen reagent (NaCl) and subsequently dipping in PVA choices. The clear MCC paper reveals extreme porosity (as a lot as 90%), adjustable pore measurement (between 23 μm and 46 μm) and large thickness (from 315 μm to 436 μm) and extreme delicate transmission beneath water (>95%).

  • By way of further modification of clear MCC paper with PVA, the obtained clear MCC/PVA paper reveals enhanced mechanical properties (dry and moist strengths), good hydrophilicity (with a contact angle of 70.8°) and improved biocompatibility (cell viability as a lot as 90%). By stacking and destacking numerous layers of the clear MCC/PVA paper, it has been used for every 2D and 3D cell custom platforms.
  • The clear MCC/PVA paper beneath water permits every direct assertion of cell morphology by optical microscope by the use of naked eyes and fluorescence microscope after staining. We envision that the developed clear MCC/PVA paper holds good potential for future functions in quite a few bioanalytical and biomedical fields, akin to drug screening, tissue engineering and organ-on-chips.
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Nature-Equal Compounds and Pure Acids Ameliorate and Cease the Damages Induced by an Inflammatory Drawback in Caco-2 CellCulture

Bioactive compounds, akin to pure acids (OA) and nature-identical compounds (NIC), can exert a job throughout the security of intestinal mucosa efficiency attributable to their natural properties.

The aim of this study was to know the perform of two OA (citric and sorbic acid) and a few NIC (thymol and vanillin), alone or blended in a mixture (OA + NIC), on intestinal barrier efficiency, each all through homeostatic state of affairs or all through an inflammatory downside carried out with pro-inflammatory cytokines and lipopolysaccharides (LPS). The study was carried out on the human epithelial cell line Caco-2, a well-known model of the intestinal epithelial barrier.

The outcomes confirmed how OA and NIC alone can improve transepithelial electrical resistance (TEER) and mRNA ranges of tight junction (TJ) components, nevertheless OA + NIC confirmed stronger efficacy compared with the single molecules.

When an inflammatory downside occurred, OA + NIC combine was able every to ameliorate, and cease, damage attributable to the pro-inflammatory stimulus, lowering or stopping the drop in TEER and enhancing the TJ mRNA expression. The data help the perform of OA + NIC in modulating gut barrier efficiency and lowering the antagonistic outcomes of irritation in intestinal epithelial cells, thereby supporting the gut barrier efficiency.

Mounting Medium
4300 3 ml
EUR 216.6
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein.

DC MEDIUM
D04-117-10kg 10 kg
EUR 1894.8

DC MEDIUM
D04-117-2kg 2kg
EUR 458.4

DC MEDIUM
D04-117-500g 500 g
EUR 168

Advanced Medium
C0003-04 RT 500 mL Bottle
EUR 123.6

Medium 199
C0012-01 RT 500 mL Bottle
EUR 126

COLIFORM MEDIUM
C03-127-10kg 10 kg
EUR 1588.8

COLIFORM MEDIUM
C03-127-2kg 2kg
EUR 392.4

COLIFORM MEDIUM
C03-127-500g 500 g
EUR 150

Heller's Medium
CP014-010 10X1L
EUR 118.8

Heller's Medium
CP014-500 50L
EUR 151.2

Hoagland's Medium
CP015-010 10X1L
EUR 118.8

Hoagland's Medium
CP015-500 50L
EUR 151.2

A Medium
DJ1018 100g
EUR 101.76

EC MEDIUM
E05-100-10kg 10 kg
EUR 976.8

EC MEDIUM
E05-100-2Kg 2 Kg
EUR 259.2

EC MEDIUM
E05-100-500g 500 g
EUR 114

NeuroProgenitor Medium
NM42400 125 ml
EUR 364.8

HLP MEDIUM
H08-107-10kg 10 kg
EUR 2733.6

HLP MEDIUM
H08-107-2Kg 2 Kg
EUR 640.8

HLP MEDIUM
H08-107-500g 500 g
EUR 218.4

M9 Medium
SD7024 250g
EUR 86.1

M9CA Medium
SD7025 250g
EUR 86.1

YM Medium
SD7031 250g
EUR 84.54

TYGPN Medium
SD7032 250g
EUR 84.01

M63 Medium
SD7033 250g
EUR 86.1

SIM MEDIUM
S19-110-10kg 10 kg
EUR 1225.2

SIM MEDIUM
S19-110-2kg 2kg
EUR 313.2

SIM MEDIUM
S19-110-500g 500 g
EUR 128.4

SOB MEDIUM
S19-124-10kg 10 kg
EUR 1158

SOB MEDIUM
S19-124-2kg 2kg
EUR 298.8

SOB MEDIUM
S19-124-500g 500 g
EUR 124.8

TCBS Medium
MED1188 PK10
EUR 7.52

DNase Medium
MED1196 PK10
EUR 7.98

OF Medium
MED1664 500G
EUR 68.4

Insect Cell Medium: TNM-FH Insect Culture Medium
ABP-MED-10001 1 liter Ask for price

Insect Cell Medium: Serum-Free Insect Culture Medium
ABP-MED-10002 1 liter Ask for price

Insect Cell Medium: Grace?s Insect Medium (Unsupplemented)
ABP-MED-10004 1 liter Ask for price

A-1 MEDIUM
A01-116-10kg 10 kg
EUR 1240.8

A-1 MEDIUM
A01-116-2Kg 2 Kg
EUR 316.8

A-1 MEDIUM
A01-116-500g 500 g
EUR 129.6

SuperMOUNT? Mounting Medium
1211-20
EUR 241.2

Recovery Medium - 6x12mL
1711 4/PK
EUR 153.6

RPMI 1640 Medium
abx098879-500ml 500 ml
EUR 210

Aqueous Mounting Medium
AR1018 10mL X5 (Enough for 800-1200 assays)
EUR 96

Antifade Mounting Medium
AR1109 10mL (for 1000 assays)
EUR 78

MCDB 131 Medium
CM034-050 500ml
EUR 102

MCDB 131 Medium
CM034-300 6x500ml
EUR 198

MCDB 131 Medium
CM034-310 10x500ml
EUR 262.8

MCDB 131 Medium
CM034-320 20x500ml
EUR 408

MCDB 131 Medium
CM034-350 50x500ml
EUR 702

Williams' Medium E
CM063-050 500ml
EUR 109.2

Williams' Medium E
CM063-300 6x500ml
EUR 319.2

Williams' Medium E
CM063-310 10x500ml
EUR 375.6

Williams' Medium E
CM063-320 20x500ml
EUR 724.8

Williams' Medium E
CM063-350 50x500ml
EUR 1170

AddexBio Cryopreservation Medium
C0002-20mL RT vial
EUR 124.8

CARY & BLAIR MEDIUM
C03-131-10kg 10 kg
EUR 2622

CARY & BLAIR MEDIUM
C03-131-2Kg 2 Kg
EUR 616.8

CARY & BLAIR MEDIUM
C03-131-500g 500 g
EUR 211.2

Cereal Crops Medium
CP003-005 5L
EUR 135.6

Cereal Crops Medium
CP003-010 10X1L
EUR 202.8

Kao Michayluk Medium
CP017-010 10X1L
EUR 118.8

Kao Michayluk Medium
CP017-500 50L
EUR 151.2

Orchid Maintenance Medium
CP037-010 10X1L
EUR 130.8

Orchid Maintenance Medium
CP037-500 50L
EUR 165.6

Woody Plants Medium
CP040-010 10X1L
EUR 121.2

Woody Plants Medium
CP040-500 50L
EUR 151.2

H Medium Broth
DJ1019 100g
EUR 73.06

EC MEDIUM, MODIFIED
E05-108-10kg 10 kg
EUR 1029.6

EC MEDIUM, MODIFIED
E05-108-2Kg 2 Kg
EUR 271.2

EC MEDIUM, MODIFIED
E05-108-500g 500 g
EUR 117.6

MOTILITY TEST MEDIUM
M13-121-10kg 10 kg
EUR 1833.6

MOTILITY TEST MEDIUM
M13-121-2Kg 2 Kg
EUR 445.2

MOTILITY TEST MEDIUM
M13-121-500g 500 g
EUR 164.4

OF BASAL MEDIUM
O15-100-10kg 10 kg Ask for price

OF BASAL MEDIUM
O15-100-2kg 2kg Ask for price

OF BASAL MEDIUM
O15-100-500g 500 g Ask for price

Hepatocyte Maintenance Medium
HM42600 100 ml
EUR 490.8

Neuron Growth Medium
HNM001 500 ml
EUR 397.2

FluoreGuard Mounting Medium
FMM030 30 ml
EUR 115.2

FluoreGuard Mounting Medium
FMM060 60 ml
EUR 139.2

FluoreGuard Mounting Medium
FMM500 500 ml
EUR 392.4

FluoreGuard Mounting Medium
FMM999 1000 ml
EUR 636

Agarose, medium EEO
GE4750-100G 100 g
EUR 199.2

Agarose, medium EEO
GE4750-500G 500 g
EUR 514.8

FLUID THIOGLYCOLLATE MEDIUM
F06-101-10kg 10 kg
EUR 950.4

FLUID THIOGLYCOLLATE MEDIUM
F06-101-2kg 2kg
EUR 253.2

FLUID THIOGLYCOLLATE MEDIUM
F06-101-500g 500 g
EUR 112.8

Schwann Growth Medium
SGM001 500 ml
EUR 292.8

Sepadextran-25 Medium
SD25M-100 100 g
EUR 325.2

Sepadextran-25 Medium
SD25M-500 500 g
EUR 1243.2

Sepadextran-50 Medium
SD50M-100 100 g
EUR 412.8

Sepadextran-50 Medium
SD50M-500 500 g
EUR 1542

TERRIFIC BROTH MEDIUM
T20-114-10kg 10 kg
EUR 810

TERRIFIC BROTH MEDIUM
T20-114-2kg 2kg
EUR 223.2

TERRIFIC BROTH MEDIUM
T20-114-500g 500 g
EUR 104.4

THIOGLYCOLLATE MEDIUM, BREWER
T20-118-10kg 10 kg
EUR 1636.8

THIOGLYCOLLATE MEDIUM, BREWER
T20-118-2kg 2kg
EUR 403.2

THIOGLYCOLLATE MEDIUM, BREWER
T20-118-500g 500 g
EUR 153.6

REINFORCED CLOSTRIDIUM MEDIUM
R18-105-10kg 10 kg
EUR 1432.8

REINFORCED CLOSTRIDIUM MEDIUM
R18-105-2kg 2kg
EUR 358.8

REINFORCED CLOSTRIDIUM MEDIUM
R18-105-500g 500 g
EUR 141.6

10mL S.O.C. Medium
46-003-CR PK10
EUR 110.58

Ec Medium 500gm
231430 EACH
EUR 149.34

Difco 2xYT Medium
244020 EACH
EUR 118.56

Sob Medium 500gm
244310 EACH
EUR 234.84

Legionella GVPC medium
257007 PK20
EUR 99.18

Lowenstein-Jensen Medium
MED1400 EACH
EUR 114
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