
A Kinetic Pump Integrated Microfluidic Plate (KIM-Plate) with High Usability for Cell Culture-Based Multiorgan Microphysiological Systems
- This examine presents the development and validation of a physiologically related triple-cell co-culture mannequin that was used to research the enter of iron in HCV an infection and the interaction between HCV, iron, and determinants of host innate immunity.
- We recorded the expression patterns of key proteins of iron homeostasis concerned in iron import, export and storage and examined their relation to the iron regulatory hormone hepcidin in hepatocytes, enterocytes and macrophages within the presence and absence of HCV.
- We then assessed the transcriptional profiles of pro-inflammatory cytokines Interleukin-6 (IL-6) and interleukin-15 (IL-15) and anti inflammatory interleukin-10 (IL-10) below regular or iron-depleted circumstances and decided how these have been affected by an infection.
- Our knowledge counsel the presence of a hyperlink between iron homeostasis and innate immunity unfolding amongst liver, gut, and macrophages, which may take part within the deregulation of innate immune responses noticed in early HCV an infection.
- Coupled with iron-assisted enhanced viral propagation, such a mechanism could also be vital for the institution of viral persistence and the following continual liver illness.
Bacterial CellCultures in a Lab-on-a-Disc: a Simple and Versatile System for Quantification of Antibiotic Treatment Efficacy
Pathogenic bacterial biofilms might be life threatening, tremendously decrease affected individual’s prime quality of life and are a substantial burden on the healthcare system. Current methods for evaluation of antibacterial therapies in clinics and in vitro strategies utilized in drug development and screening each do not facilitate biofilm formation or are cumbersome to perform, need large reagent volumes and are costly, limiting their usability.
To deal with these factors, this work presents the occasion of a sturdy in vitro cell custom platform appropriate with confocal microscopy.
The platform fashioned as a compact disc, facilitates long term bacterial custom with out exterior pumps and tubing and might be operated for numerous days with out additional liquid coping with. For example, Pseudomonas aeruginosa biofilm is grown from single cells and it is confirmed that:
1) the platform delivers reproducible and reliable outcomes; 2) progress depends on motion cost and progress medium composition; and three) effi-cacy of antibiotic treatment will rely upon the formed biofilm. This platform permits biofilm progress, quantification and treatment as in a standard motion setup, whereas decreasing the equipment barrier of lab-on-chip strategies. It offers an easy-to-use, moderately priced chance for end prospects working with cell culturing in relation to e.g. diagnostics and drug screening.
Clear Microcrystalline Cellulose/Polyvinyl Alcohol Paper as New Platform for 3D cell tradition
Multilayered and stacked cellulose paper has emerged as a promising platform for constructing of three-dimensional (3D) cell custom attributable to its low value, good biocompatibility and extreme porosity. Nonetheless, its poor delicate transmission makes it tough to immediately and clearly monitor cell behaviors (e.g., progress and proliferation) on the paper-based platform using optical microscope.
On this work, we developed a transparent microcrystalline cellulose/polyvinyl alcohol (MCC/PVA) paper with irregular pores through dissolution and regeneration of microcrystalline nanocellulose (MCC), addition of porogen reagent (NaCl) and subsequently dipping in PVA choices. The clear MCC paper reveals extreme porosity (as a lot as 90%), adjustable pore measurement (between 23 μm and 46 μm) and large thickness (from 315 μm to 436 μm) and extreme delicate transmission beneath water (>95%).
- By way of further modification of clear MCC paper with PVA, the obtained clear MCC/PVA paper reveals enhanced mechanical properties (dry and moist strengths), good hydrophilicity (with a contact angle of 70.8°) and improved biocompatibility (cell viability as a lot as 90%). By stacking and destacking numerous layers of the clear MCC/PVA paper, it has been used for every 2D and 3D cell custom platforms.
- The clear MCC/PVA paper beneath water permits every direct assertion of cell morphology by optical microscope by the use of naked eyes and fluorescence microscope after staining. We envision that the developed clear MCC/PVA paper holds good potential for future functions in quite a few bioanalytical and biomedical fields, akin to drug screening, tissue engineering and organ-on-chips.

Nature-Equal Compounds and Pure Acids Ameliorate and Cease the Damages Induced by an Inflammatory Drawback in Caco-2 CellCulture
Bioactive compounds, akin to pure acids (OA) and nature-identical compounds (NIC), can exert a job throughout the security of intestinal mucosa efficiency attributable to their natural properties.
The aim of this study was to know the perform of two OA (citric and sorbic acid) and a few NIC (thymol and vanillin), alone or blended in a mixture (OA + NIC), on intestinal barrier efficiency, each all through homeostatic state of affairs or all through an inflammatory downside carried out with pro-inflammatory cytokines and lipopolysaccharides (LPS). The study was carried out on the human epithelial cell line Caco-2, a well-known model of the intestinal epithelial barrier.
The outcomes confirmed how OA and NIC alone can improve transepithelial electrical resistance (TEER) and mRNA ranges of tight junction (TJ) components, nevertheless OA + NIC confirmed stronger efficacy compared with the single molecules.
When an inflammatory downside occurred, OA + NIC combine was able every to ameliorate, and cease, damage attributable to the pro-inflammatory stimulus, lowering or stopping the drop in TEER and enhancing the TJ mRNA expression. The data help the perform of OA + NIC in modulating gut barrier efficiency and lowering the antagonistic outcomes of irritation in intestinal epithelial cells, thereby supporting the gut barrier efficiency.
![]() A Medium |
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DJ1018 | Bio Basic | 100g | EUR 84.8 |
![]() Advanced Medium |
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C0003-04 | Addexbio | RT 500 mL Bottle | EUR 103 |
![]() Medium 199 |
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C0012-01 | Addexbio | RT 500 mL Bottle | EUR 105 |
![]() Heller's Medium |
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CP014-010 | GenDepot | 10X1L | EUR 99 |
![]() Heller's Medium |
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CP014-500 | GenDepot | 50L | EUR 126 |
![]() Hoagland's Medium |
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CP015-010 | GenDepot | 10X1L | EUR 99 |
![]() Hoagland's Medium |
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CP015-500 | GenDepot | 50L | EUR 126 |
![]() M9 Medium |
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SD7024 | Bio Basic | 250g | EUR 71.75 |
![]() M9CA Medium |
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SD7025 | Bio Basic | 250g | EUR 71.75 |
![]() YM Medium |
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SD7031 | Bio Basic | 250g | EUR 70.45 |
![]() TYGPN Medium |
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SD7032 | Bio Basic | 250g | EUR 70.01 |
![]() M63 Medium |
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SD7033 | Bio Basic | 250g | EUR 71.75 |
![]() NeuroProgenitor Medium |
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NM42400 | Neuromics | 125 ml | EUR 304 |
![]() COLIFORM MEDIUM |
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C03-127-10kg | Alphabiosciences | 10 kg | EUR 1324 |
![]() COLIFORM MEDIUM |
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C03-127-2kg | Alphabiosciences | 2kg | EUR 327 |
![]() COLIFORM MEDIUM |
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C03-127-500g | Alphabiosciences | 500 g | EUR 125 |
![]() HLP MEDIUM |
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H08-107-10kg | Alphabiosciences | 10 kg | EUR 2278 |
![]() HLP MEDIUM |
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H08-107-2Kg | Alphabiosciences | 2 Kg | EUR 534 |
![]() HLP MEDIUM |
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H08-107-500g | Alphabiosciences | 500 g | EUR 182 |
![]() EC MEDIUM |
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E05-100-10kg | Alphabiosciences | 10 kg | EUR 814 |
![]() EC MEDIUM |
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E05-100-2Kg | Alphabiosciences | 2 Kg | EUR 216 |
![]() EC MEDIUM |
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E05-100-500g | Alphabiosciences | 500 g | EUR 95 |
![]() DC MEDIUM |
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D04-117-10kg | Alphabiosciences | 10 kg | EUR 1579 |
![]() DC MEDIUM |
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D04-117-2kg | Alphabiosciences | 2kg | EUR 382 |
![]() DC MEDIUM |
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D04-117-500g | Alphabiosciences | 500 g | EUR 140 |
![]() SIM MEDIUM |
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S19-110-10kg | Alphabiosciences | 10 kg | EUR 1021 |
![]() SIM MEDIUM |
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S19-110-2kg | Alphabiosciences | 2kg | EUR 261 |
![]() SIM MEDIUM |
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S19-110-500g | Alphabiosciences | 500 g | EUR 107 |
![]() SOB MEDIUM |
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S19-124-10kg | Alphabiosciences | 10 kg | EUR 965 |
![]() SOB MEDIUM |
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S19-124-2kg | Alphabiosciences | 2kg | EUR 249 |
![]() SOB MEDIUM |
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S19-124-500g | Alphabiosciences | 500 g | EUR 104 |
![]() Insect Cell Medium: TNM-FH Insect Culture Medium |
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ABP-MED-10001 | Allele Biotech | 1 liter | Ask for price |
![]() Insect Cell Medium: Serum-Free Insect Culture Medium |
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ABP-MED-10002 | Allele Biotech | 1 liter | Ask for price |
![]() Insect Cell Medium: Grace?s Insect Medium (Unsupplemented) |
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ABP-MED-10004 | Allele Biotech | 1 liter | Ask for price |
![]() H Medium Broth |
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DJ1019 | Bio Basic | 100g | EUR 60.88 |
![]() Aqueous Mounting Medium |
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AR1018 | BosterBio | 10mL X5 (Enough for 800-1200 assays) | EUR 80 |
![]() Antifade Mounting Medium |
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AR1109 | BosterBio | 10mL (for 1000 assays) | EUR 65 |
![]() AddexBio Cryopreservation Medium |
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C0002-20mL | Addexbio | RT vial | EUR 104 |
![]() RPMI 1640 Medium |
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abx098879-500ml | Abbexa | 500 ml | EUR 175 |
![]() Recovery Medium - 6x12mL |
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1711 | Intact Genomics | 4/PK | EUR 128 |
![]() Agarose, medium EEO |
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GE4750-100G | Glentham Life Sciences | 100 g | EUR 166 |
![]() Agarose, medium EEO |
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GE4750-500G | Glentham Life Sciences | 500 g | EUR 429 |
![]() Schwann Growth Medium |
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SGM001 | Neuromics | 500 ml | EUR 244 |
![]() MCDB 131 Medium |
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CM034-050 | GenDepot | 500ml | EUR 85 |
![]() MCDB 131 Medium |
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CM034-300 | GenDepot | 6x500ml | EUR 165 |
![]() MCDB 131 Medium |
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CM034-310 | GenDepot | 10x500ml | EUR 219 |
![]() MCDB 131 Medium |
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CM034-320 | GenDepot | 20x500ml | EUR 340 |
![]() MCDB 131 Medium |
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CM034-350 | GenDepot | 50x500ml | EUR 585 |
![]() Williams' Medium E |
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CM063-050 | GenDepot | 500ml | EUR 91 |
![]() Williams' Medium E |
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CM063-300 | GenDepot | 6x500ml | EUR 266 |
![]() Williams' Medium E |
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CM063-310 | GenDepot | 10x500ml | EUR 313 |
![]() Williams' Medium E |
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CM063-320 | GenDepot | 20x500ml | EUR 604 |
![]() Williams' Medium E |
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CM063-350 | GenDepot | 50x500ml | EUR 975 |
![]() Cereal Crops Medium |
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CP003-005 | GenDepot | 5L | EUR 113 |
![]() Cereal Crops Medium |
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CP003-010 | GenDepot | 10X1L | EUR 169 |
![]() Kao Michayluk Medium |
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CP017-010 | GenDepot | 10X1L | EUR 99 |
![]() Kao Michayluk Medium |
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CP017-500 | GenDepot | 50L | EUR 126 |
![]() Orchid Maintenance Medium |
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CP037-010 | GenDepot | 10X1L | EUR 109 |
![]() Orchid Maintenance Medium |
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CP037-500 | GenDepot | 50L | EUR 138 |
![]() Woody Plants Medium |
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CP040-010 | GenDepot | 10X1L | EUR 101 |
![]() Woody Plants Medium |
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CP040-500 | GenDepot | 50L | EUR 126 |
![]() EverBrite Mounting Medium |
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23001 | Biotium | 10mL | EUR 178 |
Description: Minimum order quantity: 1 unit of 10mL |
![]() Hepatocyte Maintenance Medium |
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HM42600 | Neuromics | 100 ml | EUR 409 |
![]() Neuron Growth Medium |
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HNM001 | Neuromics | 500 ml | EUR 331 |
![]() FluoreGuard Mounting Medium |
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FMM030 | ScyTek Laboratories | 30 ml | EUR 96 |
![]() FluoreGuard Mounting Medium |
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FMM060 | ScyTek Laboratories | 60 ml | EUR 116 |
![]() FluoreGuard Mounting Medium |
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FMM500 | ScyTek Laboratories | 500 ml | EUR 327 |
![]() FluoreGuard Mounting Medium |
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FMM999 | ScyTek Laboratories | 1000 ml | EUR 530 |
![]() CARY & BLAIR MEDIUM |
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C03-131-10kg | Alphabiosciences | 10 kg | EUR 2185 |
![]() CARY & BLAIR MEDIUM |
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C03-131-2Kg | Alphabiosciences | 2 Kg | EUR 514 |
![]() CARY & BLAIR MEDIUM |
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C03-131-500g | Alphabiosciences | 500 g | EUR 176 |
![]() FLUID THIOGLYCOLLATE MEDIUM |
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F06-101-10kg | Alphabiosciences | 10 kg | EUR 792 |
![]() FLUID THIOGLYCOLLATE MEDIUM |
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F06-101-2kg | Alphabiosciences | 2kg | EUR 211 |
![]() FLUID THIOGLYCOLLATE MEDIUM |
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F06-101-500g | Alphabiosciences | 500 g | EUR 94 |
![]() EC MEDIUM, MODIFIED |
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E05-108-10kg | Alphabiosciences | 10 kg | EUR 858 |
![]() EC MEDIUM, MODIFIED |
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E05-108-2Kg | Alphabiosciences | 2 Kg | EUR 226 |
![]() EC MEDIUM, MODIFIED |
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E05-108-500g | Alphabiosciences | 500 g | EUR 98 |
![]() A-1 MEDIUM |
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A01-116-10kg | Alphabiosciences | 10 kg | EUR 1034 |
![]() A-1 MEDIUM |
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A01-116-2Kg | Alphabiosciences | 2 Kg | EUR 264 |
![]() A-1 MEDIUM |
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A01-116-500g | Alphabiosciences | 500 g | EUR 108 |
![]() SuperMOUNT? Mounting Medium |
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1211-20 | Biovision | EUR 201 |
![]() 2XYT MICROBIAL MEDIUM |
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X24-102-10kg | Alphabiosciences | 10 kg | EUR 614 |
![]() 2XYT MICROBIAL MEDIUM |
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X24-102-2Kg | Alphabiosciences | 2 Kg | EUR 173 |
![]() 2XYT MICROBIAL MEDIUM |
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X24-102-500g | Alphabiosciences | 500 g | EUR 83 |
![]() Sepadextran-25 Medium |
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SD25M-100 | ABTBeads | 100 g | EUR 271 |
![]() Sepadextran-25 Medium |
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SD25M-500 | ABTBeads | 500 g | EUR 1036 |
![]() Sepadextran-50 Medium |
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SD50M-100 | ABTBeads | 100 g | EUR 344 |
![]() Sepadextran-50 Medium |
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SD50M-500 | ABTBeads | 500 g | EUR 1285 |
![]() TERRIFIC BROTH MEDIUM |
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T20-114-10kg | Alphabiosciences | 10 kg | EUR 675 |
![]() TERRIFIC BROTH MEDIUM |
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T20-114-2kg | Alphabiosciences | 2kg | EUR 186 |
![]() TERRIFIC BROTH MEDIUM |
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T20-114-500g | Alphabiosciences | 500 g | EUR 87 |
![]() THIOGLYCOLLATE MEDIUM, BREWER |
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T20-118-10kg | Alphabiosciences | 10 kg | EUR 1364 |
![]() THIOGLYCOLLATE MEDIUM, BREWER |
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T20-118-2kg | Alphabiosciences | 2kg | EUR 336 |
![]() THIOGLYCOLLATE MEDIUM, BREWER |
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T20-118-500g | Alphabiosciences | 500 g | EUR 128 |