
A Kinetic Pump Integrated Microfluidic Plate (KIM-Plate) with High Usability for Cell Culture-Based Multiorgan Microphysiological Systems
- This examine presents the development and validation of a physiologically related triple-cell co-culture mannequin that was used to research the enter of iron in HCV an infection and the interaction between HCV, iron, and determinants of host innate immunity.
- We recorded the expression patterns of key proteins of iron homeostasis concerned in iron import, export and storage and examined their relation to the iron regulatory hormone hepcidin in hepatocytes, enterocytes and macrophages within the presence and absence of HCV.
- We then assessed the transcriptional profiles of pro-inflammatory cytokines Interleukin-6 (IL-6) and interleukin-15 (IL-15) and anti inflammatory interleukin-10 (IL-10) below regular or iron-depleted circumstances and decided how these have been affected by an infection.
- Our knowledge counsel the presence of a hyperlink between iron homeostasis and innate immunity unfolding amongst liver, gut, and macrophages, which may take part within the deregulation of innate immune responses noticed in early HCV an infection.
- Coupled with iron-assisted enhanced viral propagation, such a mechanism could also be vital for the institution of viral persistence and the following continual liver illness.
Bacterial CellCultures in a Lab-on-a-Disc: a Simple and Versatile System for Quantification of Antibiotic Treatment Efficacy
Pathogenic bacterial biofilms might be life threatening, tremendously decrease affected individual’s prime quality of life and are a substantial burden on the healthcare system. Current methods for evaluation of antibacterial therapies in clinics and in vitro strategies utilized in drug development and screening each do not facilitate biofilm formation or are cumbersome to perform, need large reagent volumes and are costly, limiting their usability.
To deal with these factors, this work presents the occasion of a sturdy in vitro cell custom platform appropriate with confocal microscopy.
The platform fashioned as a compact disc, facilitates long term bacterial custom with out exterior pumps and tubing and might be operated for numerous days with out additional liquid coping with. For example, Pseudomonas aeruginosa biofilm is grown from single cells and it is confirmed that:
1) the platform delivers reproducible and reliable outcomes; 2) progress depends on motion cost and progress medium composition; and three) effi-cacy of antibiotic treatment will rely upon the formed biofilm. This platform permits biofilm progress, quantification and treatment as in a standard motion setup, whereas decreasing the equipment barrier of lab-on-chip strategies. It offers an easy-to-use, moderately priced chance for end prospects working with cell culturing in relation to e.g. diagnostics and drug screening.
Clear Microcrystalline Cellulose/Polyvinyl Alcohol Paper as New Platform for 3D cell tradition
Multilayered and stacked cellulose paper has emerged as a promising platform for constructing of three-dimensional (3D) cell custom attributable to its low value, good biocompatibility and extreme porosity. Nonetheless, its poor delicate transmission makes it tough to immediately and clearly monitor cell behaviors (e.g., progress and proliferation) on the paper-based platform using optical microscope.
On this work, we developed a transparent microcrystalline cellulose/polyvinyl alcohol (MCC/PVA) paper with irregular pores through dissolution and regeneration of microcrystalline nanocellulose (MCC), addition of porogen reagent (NaCl) and subsequently dipping in PVA choices. The clear MCC paper reveals extreme porosity (as a lot as 90%), adjustable pore measurement (between 23 μm and 46 μm) and large thickness (from 315 μm to 436 μm) and extreme delicate transmission beneath water (>95%).
- By way of further modification of clear MCC paper with PVA, the obtained clear MCC/PVA paper reveals enhanced mechanical properties (dry and moist strengths), good hydrophilicity (with a contact angle of 70.8°) and improved biocompatibility (cell viability as a lot as 90%). By stacking and destacking numerous layers of the clear MCC/PVA paper, it has been used for every 2D and 3D cell custom platforms.
- The clear MCC/PVA paper beneath water permits every direct assertion of cell morphology by optical microscope by the use of naked eyes and fluorescence microscope after staining. We envision that the developed clear MCC/PVA paper holds good potential for future functions in quite a few bioanalytical and biomedical fields, akin to drug screening, tissue engineering and organ-on-chips.

Nature-Equal Compounds and Pure Acids Ameliorate and Cease the Damages Induced by an Inflammatory Drawback in Caco-2 CellCulture
Bioactive compounds, akin to pure acids (OA) and nature-identical compounds (NIC), can exert a job throughout the security of intestinal mucosa efficiency attributable to their natural properties.
The aim of this study was to know the perform of two OA (citric and sorbic acid) and a few NIC (thymol and vanillin), alone or blended in a mixture (OA + NIC), on intestinal barrier efficiency, each all through homeostatic state of affairs or all through an inflammatory downside carried out with pro-inflammatory cytokines and lipopolysaccharides (LPS). The study was carried out on the human epithelial cell line Caco-2, a well-known model of the intestinal epithelial barrier.
The outcomes confirmed how OA and NIC alone can improve transepithelial electrical resistance (TEER) and mRNA ranges of tight junction (TJ) components, nevertheless OA + NIC confirmed stronger efficacy compared with the single molecules.
When an inflammatory downside occurred, OA + NIC combine was able every to ameliorate, and cease, damage attributable to the pro-inflammatory stimulus, lowering or stopping the drop in TEER and enhancing the TJ mRNA expression. The data help the perform of OA + NIC in modulating gut barrier efficiency and lowering the antagonistic outcomes of irritation in intestinal epithelial cells, thereby supporting the gut barrier efficiency.
![]() A Medium |
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DJ1018 | Bio Basic | 100g | EUR 101.76 |
![]() DC MEDIUM |
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D04-117-10kg | Alphabiosciences | 10 kg | EUR 1894.8 |
![]() DC MEDIUM |
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D04-117-2kg | Alphabiosciences | 2kg | EUR 458.4 |
![]() DC MEDIUM |
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D04-117-500g | Alphabiosciences | 500 g | EUR 168 |
![]() Advanced Medium |
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C0003-04 | Addexbio | RT 500 mL Bottle | EUR 123.6 |
![]() Medium 199 |
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C0012-01 | Addexbio | RT 500 mL Bottle | EUR 126 |
![]() COLIFORM MEDIUM |
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C03-127-10kg | Alphabiosciences | 10 kg | EUR 1588.8 |
![]() COLIFORM MEDIUM |
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C03-127-2kg | Alphabiosciences | 2kg | EUR 392.4 |
![]() COLIFORM MEDIUM |
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C03-127-500g | Alphabiosciences | 500 g | EUR 150 |
![]() Heller's Medium |
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CP014-010 | GenDepot | 10X1L | EUR 118.8 |
![]() Heller's Medium |
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CP014-500 | GenDepot | 50L | EUR 151.2 |
![]() Hoagland's Medium |
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CP015-010 | GenDepot | 10X1L | EUR 118.8 |
![]() Hoagland's Medium |
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CP015-500 | GenDepot | 50L | EUR 151.2 |
![]() EC MEDIUM |
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E05-100-10kg | Alphabiosciences | 10 kg | EUR 976.8 |
![]() EC MEDIUM |
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E05-100-2Kg | Alphabiosciences | 2 Kg | EUR 259.2 |
![]() EC MEDIUM |
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E05-100-500g | Alphabiosciences | 500 g | EUR 114 |
![]() Mounting Medium |
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4300 | Virostat | 3 ml | EUR 216.6 |
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein. |
![]() NeuroProgenitor Medium |
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NM42400 | Neuromics | 125 ml | EUR 364.8 |
![]() HLP MEDIUM |
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H08-107-10kg | Alphabiosciences | 10 kg | EUR 2733.6 |
![]() HLP MEDIUM |
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H08-107-2Kg | Alphabiosciences | 2 Kg | EUR 640.8 |
![]() HLP MEDIUM |
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H08-107-500g | Alphabiosciences | 500 g | EUR 218.4 |
![]() SIM MEDIUM |
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S19-110-10kg | Alphabiosciences | 10 kg | EUR 1225.2 |
![]() SIM MEDIUM |
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S19-110-2kg | Alphabiosciences | 2kg | EUR 313.2 |
![]() SIM MEDIUM |
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S19-110-500g | Alphabiosciences | 500 g | EUR 128.4 |
![]() SOB MEDIUM |
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S19-124-10kg | Alphabiosciences | 10 kg | EUR 1158 |
![]() SOB MEDIUM |
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S19-124-2kg | Alphabiosciences | 2kg | EUR 298.8 |
![]() SOB MEDIUM |
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S19-124-500g | Alphabiosciences | 500 g | EUR 124.8 |
![]() M9 Medium |
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SD7024 | Bio Basic | 250g | EUR 86.1 |
![]() M9CA Medium |
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SD7025 | Bio Basic | 250g | EUR 86.1 |
![]() YM Medium |
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SD7031 | Bio Basic | 250g | EUR 84.54 |
![]() TYGPN Medium |
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SD7032 | Bio Basic | 250g | EUR 84.01 |
![]() M63 Medium |
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SD7033 | Bio Basic | 250g | EUR 86.1 |
![]() TCBS Medium |
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MED1188 | Scientific Laboratory Supplies | PK10 | EUR 7.52 |
![]() DNase Medium |
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MED1196 | Scientific Laboratory Supplies | PK10 | EUR 7.98 |
![]() OF Medium |
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MED1664 | Scientific Laboratory Supplies | 500G | EUR 68.4 |
![]() Angiotensin III (Ang III) Antibody |
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20-abx101595 | Abbexa |
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![]() Insect Cell Medium: TNM-FH Insect Culture Medium |
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ABP-MED-10001 | Allele Biotech | 1 liter | Ask for price |
![]() Insect Cell Medium: Serum-Free Insect Culture Medium |
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ABP-MED-10002 | Allele Biotech | 1 liter | Ask for price |
![]() Insect Cell Medium: Grace?s Insect Medium (Unsupplemented) |
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ABP-MED-10004 | Allele Biotech | 1 liter | Ask for price |
![]() MCDB 131 Medium |
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CM034-050 | GenDepot | 500ml | EUR 102 |
![]() MCDB 131 Medium |
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CM034-300 | GenDepot | 6x500ml | EUR 198 |
![]() MCDB 131 Medium |
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CM034-310 | GenDepot | 10x500ml | EUR 262.8 |
![]() MCDB 131 Medium |
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CM034-320 | GenDepot | 20x500ml | EUR 408 |
![]() MCDB 131 Medium |
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CM034-350 | GenDepot | 50x500ml | EUR 702 |
![]() Williams' Medium E |
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CM063-050 | GenDepot | 500ml | EUR 109.2 |
![]() Williams' Medium E |
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CM063-300 | GenDepot | 6x500ml | EUR 319.2 |
![]() Williams' Medium E |
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CM063-310 | GenDepot | 10x500ml | EUR 375.6 |
![]() Williams' Medium E |
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CM063-320 | GenDepot | 20x500ml | EUR 724.8 |
![]() Williams' Medium E |
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CM063-350 | GenDepot | 50x500ml | EUR 1170 |
![]() H Medium Broth |
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DJ1019 | Bio Basic | 100g | EUR 73.06 |
![]() AddexBio Cryopreservation Medium |
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C0002-20mL | Addexbio | RT vial | EUR 124.8 |
![]() CARY & BLAIR MEDIUM |
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C03-131-10kg | Alphabiosciences | 10 kg | EUR 2622 |
![]() CARY & BLAIR MEDIUM |
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C03-131-2Kg | Alphabiosciences | 2 Kg | EUR 616.8 |
![]() CARY & BLAIR MEDIUM |
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C03-131-500g | Alphabiosciences | 500 g | EUR 211.2 |
![]() Cereal Crops Medium |
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CP003-005 | GenDepot | 5L | EUR 135.6 |
![]() Cereal Crops Medium |
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CP003-010 | GenDepot | 10X1L | EUR 202.8 |
![]() Kao Michayluk Medium |
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CP017-010 | GenDepot | 10X1L | EUR 118.8 |
![]() Kao Michayluk Medium |
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CP017-500 | GenDepot | 50L | EUR 151.2 |
![]() Orchid Maintenance Medium |
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CP037-010 | GenDepot | 10X1L | EUR 130.8 |
![]() Orchid Maintenance Medium |
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CP037-500 | GenDepot | 50L | EUR 165.6 |
![]() Woody Plants Medium |
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CP040-010 | GenDepot | 10X1L | EUR 121.2 |
![]() Woody Plants Medium |
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CP040-500 | GenDepot | 50L | EUR 151.2 |
![]() EC MEDIUM, MODIFIED |
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E05-108-10kg | Alphabiosciences | 10 kg | EUR 1029.6 |
![]() EC MEDIUM, MODIFIED |
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E05-108-2Kg | Alphabiosciences | 2 Kg | EUR 271.2 |
![]() EC MEDIUM, MODIFIED |
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E05-108-500g | Alphabiosciences | 500 g | EUR 117.6 |
![]() FLUID THIOGLYCOLLATE MEDIUM |
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F06-101-10kg | Alphabiosciences | 10 kg | EUR 950.4 |
![]() FLUID THIOGLYCOLLATE MEDIUM |
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F06-101-2kg | Alphabiosciences | 2kg | EUR 253.2 |
![]() FLUID THIOGLYCOLLATE MEDIUM |
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F06-101-500g | Alphabiosciences | 500 g | EUR 112.8 |
![]() FluoreGuard Mounting Medium |
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FMM030 | ScyTek Laboratories | 30 ml | EUR 115.2 |
![]() FluoreGuard Mounting Medium |
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FMM060 | ScyTek Laboratories | 60 ml | EUR 139.2 |
![]() FluoreGuard Mounting Medium |
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FMM500 | ScyTek Laboratories | 500 ml | EUR 392.4 |
![]() FluoreGuard Mounting Medium |
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FMM999 | ScyTek Laboratories | 1000 ml | EUR 636 |
![]() Recovery Medium - 6x12mL |
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1711 | Intact Genomics | 4/PK | EUR 153.6 |
![]() A-1 MEDIUM |
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A01-116-10kg | Alphabiosciences | 10 kg | EUR 1240.8 |
![]() A-1 MEDIUM |
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A01-116-2Kg | Alphabiosciences | 2 Kg | EUR 316.8 |
![]() A-1 MEDIUM |
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A01-116-500g | Alphabiosciences | 500 g | EUR 129.6 |
![]() Aqueous Mounting Medium |
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AR1018 | BosterBio | 10mL X5 (Enough for 800-1200 assays) | EUR 96 |
![]() Antifade Mounting Medium |
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AR1109 | BosterBio | 10mL (for 1000 assays) | EUR 78 |
![]() MOTILITY TEST MEDIUM |
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M13-121-10kg | Alphabiosciences | 10 kg | EUR 1833.6 |
![]() MOTILITY TEST MEDIUM |
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M13-121-2Kg | Alphabiosciences | 2 Kg | EUR 445.2 |
![]() MOTILITY TEST MEDIUM |
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M13-121-500g | Alphabiosciences | 500 g | EUR 164.4 |
![]() OF BASAL MEDIUM |
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O15-100-10kg | Alphabiosciences | 10 kg | Ask for price |
![]() OF BASAL MEDIUM |
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O15-100-2kg | Alphabiosciences | 2kg | Ask for price |
![]() OF BASAL MEDIUM |
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O15-100-500g | Alphabiosciences | 500 g | Ask for price |
![]() Hepatocyte Maintenance Medium |
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HM42600 | Neuromics | 100 ml | EUR 490.8 |
![]() Neuron Growth Medium |
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HNM001 | Neuromics | 500 ml | EUR 397.2 |
![]() Agarose, medium EEO |
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GE4750-100G | Glentham Life Sciences | 100 g | EUR 199.2 |
![]() Agarose, medium EEO |
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GE4750-500G | Glentham Life Sciences | 500 g | EUR 514.8 |
![]() REINFORCED CLOSTRIDIUM MEDIUM |
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R18-105-10kg | Alphabiosciences | 10 kg | EUR 1432.8 |
![]() REINFORCED CLOSTRIDIUM MEDIUM |
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R18-105-2kg | Alphabiosciences | 2kg | EUR 358.8 |
![]() REINFORCED CLOSTRIDIUM MEDIUM |
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R18-105-500g | Alphabiosciences | 500 g | EUR 141.6 |
![]() Schwann Growth Medium |
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SGM001 | Neuromics | 500 ml | EUR 292.8 |
![]() Sepadextran-25 Medium |
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SD25M-100 | ABTBeads | 100 g | EUR 325.2 |
![]() Sepadextran-25 Medium |
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SD25M-500 | ABTBeads | 500 g | EUR 1243.2 |
![]() Sepadextran-50 Medium |
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SD50M-100 | ABTBeads | 100 g | EUR 412.8 |
![]() Sepadextran-50 Medium |
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SD50M-500 | ABTBeads | 500 g | EUR 1542 |
![]() TERRIFIC BROTH MEDIUM |
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T20-114-10kg | Alphabiosciences | 10 kg | EUR 810 |
![]() TERRIFIC BROTH MEDIUM |
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T20-114-2kg | Alphabiosciences | 2kg | EUR 223.2 |
![]() TERRIFIC BROTH MEDIUM |
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T20-114-500g | Alphabiosciences | 500 g | EUR 104.4 |
![]() THIOGLYCOLLATE MEDIUM, BREWER |
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T20-118-10kg | Alphabiosciences | 10 kg | EUR 1636.8 |
![]() THIOGLYCOLLATE MEDIUM, BREWER |
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T20-118-2kg | Alphabiosciences | 2kg | EUR 403.2 |
![]() THIOGLYCOLLATE MEDIUM, BREWER |
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T20-118-500g | Alphabiosciences | 500 g | EUR 153.6 |
![]() 2XYT MICROBIAL MEDIUM |
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X24-102-10kg | Alphabiosciences | 10 kg | EUR 736.8 |
![]() 2XYT MICROBIAL MEDIUM |
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X24-102-2Kg | Alphabiosciences | 2 Kg | EUR 207.6 |
![]() 2XYT MICROBIAL MEDIUM |
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X24-102-500g | Alphabiosciences | 500 g | EUR 99.6 |
![]() Lowenstein-Jensen Medium |
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MED1400 | Scientific Laboratory Supplies | EACH | EUR 114 |
![]() Sabouraud Liquid Medium |
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MED1462 | Scientific Laboratory Supplies | EACH | EUR 54.61 |
![]() Slanetz & Bartley Medium |
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MED1468 | Scientific Laboratory Supplies | EACH | EUR 107.16 |
![]() Dermatophyte Test Medium |
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MED1484 | Scientific Laboratory Supplies | EACH | EUR 87.78 |