cellexusinc

A Kinetic Pump Integrated Microfluidic Plate (KIM-Plate) with High Usability for Cell Culture-Based Multiorgan Microphysiological Systems

Microphysiological programs (MPSs), together with organ-on-a-chip (OoC), have attracted consideration as a novel technique for estimating the consequences and uncomfortable side effects of medication in drug discovery. To breed the dynamic in vivo atmosphere, earlier MPSs have been linked to pump programs to perfuse tradition medium. Due to this fact, most MPSs usually are not user-friendly and have poor throughput. We aimed to develop a kinetic pump built-in microfluidic plate (KIM-Plate) by making use of the stirrer-based micropump to an open entry tradition plate to enhance the usability of MPSs.
The KIM-Plate integrates six multiorgan MPS (MO-MPS) items and meets the ANSI/SBS microplate requirements. We evaluated the perfusion perform of the kinetic pump and located that the KIM-Plate had enough agitation impact. Coculture experiments with PXB cells and hiPS intestinal cells confirmed that the TEER of hiPS intestinal cells and gene expression ranges associated to the metabolism of PXB cells have been elevated. Therefore, the KIM-Plate is an modern instrument for the straightforward coculture of extremely conditioned cells that’s anticipated to facilitate cell-based assays within the fields of drug discovery and biology due to its usability and excessive throughput nature.
Iron is essential to the regulation of the host innate immune system and the result of many infections. Hepatitis C virus (HCV), one of many main viral human pathogens that is dependent upon iron to finish its life cycle, is extremely expert in evading the immune system.
  • This examine presents the development and validation of a physiologically related triple-cell co-culture mannequin that was used to research the enter of iron in HCV an infection and the interaction between HCV, iron, and determinants of host innate immunity.
  • We recorded the expression patterns of key proteins of iron homeostasis concerned in iron import, export and storage and examined their relation to the iron regulatory hormone hepcidin in hepatocytes, enterocytes and macrophages within the presence and absence of HCV.
  • We then assessed the transcriptional profiles of pro-inflammatory cytokines Interleukin-6 (IL-6) and interleukin-15 (IL-15) and anti inflammatory interleukin-10 (IL-10) below regular or iron-depleted circumstances and decided how these have been affected by an infection.
  • Our knowledge counsel the presence of a hyperlink between iron homeostasis and innate immunity unfolding amongst liver, gut, and macrophages, which may take part within the deregulation of innate immune responses noticed in early HCV an infection.
  • Coupled with iron-assisted enhanced viral propagation, such a mechanism could also be vital for the institution of viral persistence and the following continual liver illness.

Bacterial CellCultures in a Lab-on-a-Disc: a Simple and Versatile System for Quantification of Antibiotic Treatment Efficacy

Pathogenic bacterial biofilms might be life threatening, tremendously decrease affected individual’s prime quality of life and are a substantial burden on the healthcare system. Current methods for evaluation of antibacterial therapies in clinics and in vitro strategies utilized in drug development and screening each do not facilitate biofilm formation or are cumbersome to perform, need large reagent volumes and are costly, limiting their usability.

To deal with these factors, this work presents the occasion of a sturdy in vitro cell custom platform appropriate with confocal microscopy.

The platform fashioned as a compact disc, facilitates long term bacterial custom with out exterior pumps and tubing and might be operated for numerous days with out additional liquid coping with. For example, Pseudomonas aeruginosa biofilm is grown from single cells and it is confirmed that:

1) the platform delivers reproducible and reliable outcomes; 2) progress depends on motion cost and progress medium composition; and three) effi-cacy of antibiotic treatment will rely upon the formed biofilm. This platform permits biofilm progress, quantification and treatment as in a standard motion setup, whereas decreasing the equipment barrier of lab-on-chip strategies. It offers an easy-to-use, moderately priced chance for end prospects working with cell culturing in relation to e.g. diagnostics and drug screening.

Clear Microcrystalline Cellulose/Polyvinyl Alcohol Paper as New Platform for 3D cell tradition

Multilayered and stacked cellulose paper has emerged as a promising platform for constructing of three-dimensional (3D) cell custom attributable to its low value, good biocompatibility and extreme porosity. Nonetheless, its poor delicate transmission makes it tough to immediately and clearly monitor cell behaviors (e.g., progress and proliferation) on the paper-based platform using optical microscope.

On this work, we developed a transparent microcrystalline cellulose/polyvinyl alcohol (MCC/PVA) paper with irregular pores through dissolution and regeneration of microcrystalline nanocellulose (MCC), addition of porogen reagent (NaCl) and subsequently dipping in PVA choices. The clear MCC paper reveals extreme porosity (as a lot as 90%), adjustable pore measurement (between 23 μm and 46 μm) and large thickness (from 315 μm to 436 μm) and extreme delicate transmission beneath water (>95%).

  • By way of further modification of clear MCC paper with PVA, the obtained clear MCC/PVA paper reveals enhanced mechanical properties (dry and moist strengths), good hydrophilicity (with a contact angle of 70.8°) and improved biocompatibility (cell viability as a lot as 90%). By stacking and destacking numerous layers of the clear MCC/PVA paper, it has been used for every 2D and 3D cell custom platforms.
  • The clear MCC/PVA paper beneath water permits every direct assertion of cell morphology by optical microscope by the use of naked eyes and fluorescence microscope after staining. We envision that the developed clear MCC/PVA paper holds good potential for future functions in quite a few bioanalytical and biomedical fields, akin to drug screening, tissue engineering and organ-on-chips.
cellexusinc
cellexusinc

Nature-Equal Compounds and Pure Acids Ameliorate and Cease the Damages Induced by an Inflammatory Drawback in Caco-2 CellCulture

Bioactive compounds, akin to pure acids (OA) and nature-identical compounds (NIC), can exert a job throughout the security of intestinal mucosa efficiency attributable to their natural properties.

The aim of this study was to know the perform of two OA (citric and sorbic acid) and a few NIC (thymol and vanillin), alone or blended in a mixture (OA + NIC), on intestinal barrier efficiency, each all through homeostatic state of affairs or all through an inflammatory downside carried out with pro-inflammatory cytokines and lipopolysaccharides (LPS). The study was carried out on the human epithelial cell line Caco-2, a well-known model of the intestinal epithelial barrier.

The outcomes confirmed how OA and NIC alone can improve transepithelial electrical resistance (TEER) and mRNA ranges of tight junction (TJ) components, nevertheless OA + NIC confirmed stronger efficacy compared with the single molecules.

When an inflammatory downside occurred, OA + NIC combine was able every to ameliorate, and cease, damage attributable to the pro-inflammatory stimulus, lowering or stopping the drop in TEER and enhancing the TJ mRNA expression. The data help the perform of OA + NIC in modulating gut barrier efficiency and lowering the antagonistic outcomes of irritation in intestinal epithelial cells, thereby supporting the gut barrier efficiency.

KATO III [KATO 3] Cells Complete Medium

CM-0372-125mL4 125 mL×4
EUR 108
Description: Complete Growth Medium

Medium 199

C0012-01 RT 500 mL Bottle
EUR 126

DC MEDIUM

D04-117-10kg 10 kg
EUR 1894.8

DC MEDIUM

D04-117-2kg 2kg
EUR 458.4

DC MEDIUM

D04-117-500g 500 g
EUR 168

A Medium

DJ1018 100g
EUR 101.76

EC MEDIUM

E05-100-10kg 10 kg
EUR 976.8

EC MEDIUM

E05-100-2Kg 2 Kg
EUR 259.2

EC MEDIUM

E05-100-500g 500 g
EUR 114

OF Medium

MED1664 500G
EUR 79.2

M9 Medium

SD7024 250g
EUR 86.1

YM Medium

SD7031 250g
EUR 84.54

HLP MEDIUM

H08-107-10kg 10 kg
EUR 2733.6

HLP MEDIUM

H08-107-2Kg 2 Kg
EUR 640.8

HLP MEDIUM

H08-107-500g 500 g
EUR 218.4

M63 Medium

SD7033 250g
EUR 86.1

SIM MEDIUM

S19-110-10kg 10 kg
EUR 1225.2

SIM MEDIUM

S19-110-2kg 2kg
EUR 313.2

SIM MEDIUM

S19-110-500g 500 g
EUR 128.4

SOB MEDIUM

S19-124-10kg 10 kg
EUR 1158

SOB MEDIUM

S19-124-2kg 2kg
EUR 298.8

SOB MEDIUM

S19-124-500g 500 g
EUR 124.8

NLN Medium

30630102-2 25 L
EUR 19.28

NLN Medium

30630102-3 50 L
EUR 39.2

TAP Medium

30639002-1 100 g
EUR 48.32

TAP Medium

30639002-2 500 g
EUR 200.66

Ec Medium 500gm

231430 EACH
EUR 176.4

Rat Cartilage Growth Medium PrimaCell: Normal Articular Cartilage Growth Medium

9-25023 5 x 100 ml Ask for price

TCBS Medium

MED1188 PK10
EUR 9.6

M9CA Medium

SD7025 250g
EUR 86.1

TM4G Medium

30630177-2 25 L
EUR 22.1

TM4G Medium

30630177-3 50 L
EUR 41.14

Sob Medium 500gm

244310 EACH
EUR 261.6

Human Cartilage Growth Medium PrimaCell: Normal Articular Cartilage Growth Medium

9-46043 5 x 100 ml Ask for price

DNase Medium

MED1196 PK10
EUR 10.8

TYGPN Medium

SD7032 250g
EUR 84.01

White's Medium

30630124-3 25 L
EUR 31.67

White's Medium

30630124-4 50 L
EUR 43.46

Heller's Medium

CP014-010 10X1L
EUR 118.8

Heller's Medium

CP014-500 50L
EUR 151.2

Heller Medium

30630036-2 25 L
EUR 25.45

Heller Medium

30630036-3 50 L
EUR 43

Jensen's Medium

30630039-3 25 L
EUR 40.58

Jensen's Medium

30630039-4 50 L
EUR 77.28

Litvay Medium

30630048-3 25 L
EUR 29.72

Litvay Medium

30630048-4 50 L
EUR 40.38

Nitsch Medium

30630095-2 25 L
EUR 25.16

Nitsch Medium

30630095-3 50 L
EUR 43.37

Euglena Medium

30639001-1 500 g
EUR 86.6

RM-01 Refeed Medium (zero-Serum Refeed medium)

10-310100 100 mL Ask for price
Description: A defined medium with EMEM with EBSS, and Glutamine. Sterile.

Insect Cell Medium: TNM-FH Insect Culture Medium

ABP-MED-10001 1 liter Ask for price

Mounting Medium

4300 3 ml
EUR 216.6
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein.

Advanced Medium

C0003-04 RT 500 mL Bottle
EUR 123.6

COLIFORM MEDIUM

C03-127-10kg 10 kg
EUR 1588.8

COLIFORM MEDIUM

C03-127-2kg 2kg
EUR 392.4

COLIFORM MEDIUM

C03-127-500g 500 g
EUR 150

Hoagland's Medium

CP015-010 10X1L
EUR 118.8

Hoagland's Medium

CP015-500 50L
EUR 151.2

Orchimax Medium

30630105-3 25 L
EUR 46.37

Orchimax Medium

30630105-4 50 L
EUR 61.84

Insect Cell Medium: Serum-Free Insect Culture Medium

ABP-MED-10002 1 liter Ask for price

RPMI 1640 Medium

abx098879-500ml 500 ml
EUR 210

A-1 MEDIUM

A01-116-10kg 10 kg
EUR 1240.8

A-1 MEDIUM

A01-116-2Kg 2 Kg
EUR 316.8

A-1 MEDIUM

A01-116-500g 500 g
EUR 129.6

MCDB 131 Medium

CM034-050 500ml
EUR 102

MCDB 131 Medium

CM034-300 6x500ml
EUR 198

MCDB 131 Medium

CM034-310 10x500ml
EUR 262.8

MCDB 131 Medium

CM034-320 20x500ml
EUR 408

MCDB 131 Medium

CM034-350 50x500ml
EUR 702

Medium 199/BCDB 105

C0012-02 RT 500 mL Bottle
EUR 181.2

RM-02 Refeed Medium (Standard 2% FBS Refeed Medium)

10-320100 100 mL Ask for price
Description: used with most cell cultures for detection of viruses other than influenza

RM-02 Refeed Medium (Standard 2% FBS Refeed Medium)

10-320500 500 mL Ask for price
Description: used with most cell cultures for detection of viruses other than influenza

RM-05 Refeed Medium (Standard 10% FBS Refeed Medium)

10-350100 100 mL Ask for price
Description: used with most cell cultures for detection of viruses other than influenza

RM-05 Refeed Medium (Standard 10% FBS Refeed Medium)

10-350500 500 mL Ask for price
Description: used with most cell cultures for detection of viruses other than influenza

Insect Cell Medium: Grace?s Insect Medium (Unsupplemented)

ABP-MED-10004 1 liter Ask for price

BPS Medium 1

79259 100 ml
EUR 120
Description: This liquid BPS Medium is optimized for use with many BPS Reporter Kits including_x000D_ • 60612: AP1 Reporter Kit (JNK Pathway)_x000D_ • 60514: ARE Reporter Kit (Nrf2 Antioxidant Pathway)_x000D_ • 60611: CRE/CREB Reporter Kit (cAMP/PKA Signaling Pathway)_x000D_ • 60614: NF-κB Reporter Kit (NF-κB Signaling Pathway)_x000D_ • 60509: Notch1/CSL Reporter Kit (Notch Signaling Pathway)_x000D_ • 60654: SBE Reporter Kit (TGFβ/SMAD signaling pathway)_x000D_ • 60511: SRE Reporter Kit (MAPK/ERK Signaling Pathway)_x000D_ • 60500: TCF/LEF Reporter Kit (Wnt Signaling Pathway)

BPS Medium 7

79260 100 ml
EUR 120
Description: This liquid BPS Medium is optimized for use with select BPS reporter kits.

BPS Medium 8

79385 100 ml
EUR 120
Description: This liquid BPS Medium is optimized for use with the CSF1R/SRE Reporter Kit (BPS Bioscience, #79379).

Chu (N6) Medium

30630007-2 25 L
EUR 25.16

Chu (N6) Medium

30630007-3 50 L
EUR 31.57

Isp Medium 2 500gm

277010 EACH
EUR 356.4

Isp Medium 4 500gm

277210 EACH
EUR 340.8

Medium 199/MeT-5A

C0012-03 RT 500 mL Bottle
EUR 80

Thaw Medium 8

79652-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines including NF- κB Reporter (Luc) – THP-1 Cell Line, #79645

Thaw Medium 8

79652-2 500 ml
EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines including NF- κB Reporter (Luc) – THP-1 Cell Line, #79645

Thaw Medium 9

79665-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including_x000D_• CRE/CREB Reporter Lentivirus #79580 _x000D_• NF-κB Reporter Luciferase Reporter Lentivirus #79564

Thaw Medium 9

79665-2 500 ml
EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, using • CRE/CREB Reporter Lentivirus #79580 _x000D_• NF-κB Reporter Luciferase Reporter Lentivirus #79564

Thaw Medium 10

79704-1 100 ml
EUR 140
Description: This liquid medium is optimized to culture select BPS Bioscience cell lines.

Thaw Medium 10

79704-2 500 ml
EUR 600
Description: This liquid medium is optimized to culture select BPS Bioscience cell lines.

Thaw Medium 11

79976-1 100 ml
EUR 140
Description: This liquid medium is optimized to culture select BPS Bioscience cell lines._x000D_Optimized for Use With:_x000D_NF- κB Reporter (Luc) - Raw 264.7 Cell line (BPS Bioscience 79978)

Thaw Medium 11

79976-2 500 ml
EUR 600
Description: This liquid medium is optimized to culture select BPS Bioscience cell lines._x000D_Optimized for Use With:_x000D_NF- κB Reporter (Luc) - Raw 264.7 Cell line (BPS Bioscience 79978)

Thaw Medium 12

78074-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines, including: • Cas9-expressing MDA-MB-231 cells (BPS Bioscience #78069)

Thaw Medium 12

78074-2 500 ml
EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines, including: • Cas9-expressing MDA-MB-231 cells (BPS Bioscience #78069)

Thaw Medium 14

78397-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines.

Thaw Medium 14

78397-2 500 ml
EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines.

Thaw Medium 15

78412-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines.

Thaw Medium 15

78412-2 500 ml
EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines.

Thaw Medium 16

78647-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for use with tetracycline-inducible system.

Thaw Medium 16

78647-2 500 ml
EUR 600
Description: This liquid Cell Thawing Medium is optimized for use with tetracycline-inducible system.

Thaw Medium 4

60181-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• CFTR - HEK 293 Recombinant Cell Line #60506• Nav1.8/β2 - HEK293 Recombinant Cell Line #60521• NaV1.7-HEK293 Recombinant Cell Line #60607

Thaw Medium 4

60181-2 500 ml
EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• CFTR - HEK 293 Recombinant Cell Line #60506• Nav1.8/β2 - HEK293 Recombinant Cell Line #60521• NaV1.7-HEK293 Recombinant Cell Line #60607

Thaw Medium 5

60182-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Gli Reporter - NIH3T3 Recombinant Cell line (Hedgehog Pathway) #60409

Thaw Medium 5

60182-2 500 ml
EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Gli Reporter - NIH3T3 Recombinant Cell line (Hedgehog Pathway) #60409

Thaw Medium 6

60183-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway) #60520• NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line #60623 • RARα Reporter - HEK293 Recombinant Cell Line #60503 • RARβ Reporter - HEK293 Recombinant Cell Line #60603 • RARγ Reporter - HEK293 Reporter Cell Line #60604

Thaw Medium 6

60183-2 500 ml
EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway) #60520• NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line #60623 • RARα Reporter - HEK293 Recombinant Cell Line #60503 • RARβ Reporter - HEK293 Recombinant Cell Line #60603 • RARγ Reporter - HEK293 Reporter Cell Line #60604

Thaw Medium 2

60184-1 100 ml
EUR 150
Description: This liquid Cell Thawing Medium is optimized for thawing and plating BPS Bioscience's Jurkat-based cell lines, including• PD-1/NFAT Reporter-Jurkat cell #60535• NFAT Reporter (Luc) - Jurkat Cell Line #60621• NF-κB-Luciferase Reporter (Luc) - Jurkat Cell Line #60651• ADCC Bioassay Effector Cell F variant (Low Affinity) #60540• ADCC Bioassay Effector Cell V variant (High Affinity) #60541Note: once cells are well-established, switch to the appropriate cell growth medium.

Thaw Medium 2

60184-2 500 ml
EUR 675
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including_x000D__x000D_• PD-1/NFAT Reporter-Jurkat cell #60535_x000D__x000D_• NFAT Reporter (Luc) - Jurkat Cell Line #60621_x000D__x000D_• NF-ĸB-Luciferase Reporter (Luc) - Jurkat Cell Line #60651_x000D__x000D_• ADCC Bioassay Effector Cell F variant (Low Affinity) #60540_x000D__x000D_• ADCC Bioassay Effector Cell V variant (High Affinity) #60541_x000D__x000D_Note: once cells are well-established, switch to the appropriate cell growth medium.

Thaw Medium 7

60185-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway) #60520 • NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line #60623