
Live-cell imaging of microglial interactions with radial glia in transgenic embryonic mouse brains using slice culture
Evaluation on the prokaryotic microbiome in females and embryonic cell cultures of Rhipicephalus sanguineus tropical and temperate lineages from two particular localities in Brazil
Human umbilical cord-derived mesenchymal stem cells have an effect on urea synthesis and the cell apoptosis of human induced hepatocytes by secreting IL-6 in a serum-free co-tradition system
A protocol for analyzing the protein terminome of human most cancers cell line tradition supernatants
Improvement of 3D cultures of zebrafish liver and embryo cell strains: a comparability of various spheroid formation strategies
Fish cell spheroids are promising 3D tradition fashions for vertebrate alternative in ecotoxicology. Nonetheless, new various ecotoxicological strategies should be tailored for purposes in business and for regulatory functions; such strategies should be cost-effective, easy to control and supply speedy outcomes. Due to this fact, we in contrast the effectiveness of the normal hanging drop (HD), orbital shaking (OS), and HD mixed with OS (HD+OS) strategies on the formation of zebrafish cell line spheroids (ZFL and ZEM2S). Time in HD (3-5 days) and completely different 96-well plates [flat-bottom or ultra-low attachment of round-bottom (ULA-plates)] in OS had been evaluated. Straightforward dealing with, speedy spheroid formation, uniform-sized spheroids, and circularity had been assessed to determine the very best spheroid protocol.
Conventional HD alone didn’t lead to ZFL spheroid formation, whereas HD (5 days)+OS did. When utilizing the OS, spheroids solely shaped on the ULA-plate. Each HD+OS and OS had been reproducible in measurement (177.50 ± 2.81 µm and 225.62 ± 19.20 µm, respectively) and circularity (0.83 ± 0.02 and 0.80 ± 0.01, respectively) of ZFL spheroids. Nonetheless, HD+OS required a substantial time to utterly type spheroids (10 days) and intensive dealing with, whereas the OS was quick (5 days of incubation) and easy.
OS additionally yielded reproducible ZEM2S spheroids in 1 day (226.23 ± 0.57 µm diameter and 0.80 ± 0.01 circularity). In conclusion, OS in ULA-plate is an efficient and easy spheroid protocol for high-throughput ecotoxicity testing. This research contributes to determine a quick, reproducible, and easy protocol of single piscine spheroid formation in 96-well plates and helps the applying of fish 3D mannequin in business and academia.

Immunomodulatory potential of Clinacanthus nutans extracts within the co-tradition of triple-negative breast most cancers cells, MDA-MB-231, and THP-1 macrophages
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BSP-5Y | Next Advance | 1pack | EUR 289.2 |
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BSP-50B2 | Next Advance | 1pack | EUR 484.8 |
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BSP-ALL2 | Next Advance | 1pack | EUR 460.8 |
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BSP-OT3 | Next Advance | 1pack | EUR 282 |
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BSP-MC2 | Next Advance | 1pack | EUR 278.4 |
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W0900-050 | GenDepot | 500 ml | EUR 81.6 |
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W0900-350 | GenDepot | 50x500 ml | EUR 627.6 |
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88R-1050 | Fitzgerald | 6 x 2 ml | EUR 483.6 |
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KF17355 | Neuromics | 3X100 ml | EUR 326.4 |
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KF17340 | Neuromics | 3 x 100 ml | EUR 246 |
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KF17351 | Neuromics | 4X100 ml | EUR 326.4 |
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42R-TB101 | Fitzgerald | 3 x 100 ml | EUR 360 |
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354688 | CORNING | 3/pk | EUR 543.6 |
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PCTA100 | Plant Cell Technology | 500g | EUR 144 |
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PCTA500 | Plant Cell Technology | 1000g | EUR 180 |
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75016067 | Scientific Laboratory Supplies | EACH | EUR 16141.2 |
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75016066 | Scientific Laboratory Supplies | EACH | EUR 21742.8 |
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75016075 | Scientific Laboratory Supplies | EACH | EUR 18357.6 |
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430293 | Scientific Laboratory Supplies | PK480 | EUR 507.6 |
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TTGA-SP3-B090 | BioAssayWorks | 3 x (1 cm x 30 cm) | EUR 273.2 |
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356468 | CORNING | 20/pk | EUR 469.2 |
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CCM1081-500 | Bio Basic | 500 mL | EUR 73.49 |
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CCM2081-1000 | Bio Basic | 1000 mL | EUR 82.64 |
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ABP-RVC-10002C | Allele Biotech | 1 flask | Ask for price |
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356456 | CORNING | 20/pk | EUR 469.2 |
Description: Advanced Cells - DL; Dishes BCT |
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ABP-RVC-10001C | Allele Biotech | 1 flask | Ask for price |
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356467 | CORNING | 20/pk | EUR 468 |
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356517 | CORNING | 20/pk | EUR 481.2 |
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356518 | CORNING | 20/pk | EUR 481.2 |
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STJ16100969 | St John's Laboratory | 1 pack | EUR 799.2 |
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BLI21940-1 | Polysciences Europe GmbH | 5ml | EUR 395 |
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ExoS-25-C9 | ImmunoStep | 25 test | EUR 660.6 |
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356653 | CORNING | 10/pk | EUR 262.8 |
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354458 | CORNING | 20/pk | EUR 267.6 |
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IB42020 | IBI Scientific | 2L | EUR 59.67 |
Description: DBL DIST. - 0.1 MICRON STERILE |
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IB42021 | IBI Scientific | 6x2L | EUR 342.87 |
Description: DBL DIST. - 0.1 MICRON STERILE |
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IB42030 | IBI Scientific | 10L | EUR 194.72 |
Description: JUG W/HANDL 0.1 MICRON STERILE |
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91-200-80 | CORNING | 1/pk | EUR 450 |
Description: Flexible Containers; Bioprocess Bags and Parts |
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63-0600 | Quidel | each | Ask for price |
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91-200-78 | CORNING | 1/pk | EUR 493.2 |
Description: Flexible Containers; Bioprocess Bags and Parts |
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91-200-81 | CORNING | 1/pk | EUR 570 |
Description: Flexible Containers; Bioprocess Bags and Parts |
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M16101 | Neuromics | 500 mL | EUR 1336.8 |
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abx090688-50plates | Abbexa | 50 plates | EUR 571.2 |
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abx090688-6plates | Abbexa | 6 plates | EUR 142.8 |
![]() Sample Cell 1 Inch Round PS | |||
WAT1120 | Scientific Laboratory Supplies | PK2 | EUR 56.4 |
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K518-100 | Biovision | each | EUR 744 |
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BLI21950-1 | Polysciences Europe GmbH | 1ml | EUR 342 |
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220253 | Scientific Laboratory Supplies | EACH | EUR 57.32 |
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356469 | CORNING | 10/pk | EUR 262.8 |
Description: Advanced Cells - DL; Dishes BCT |
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Description: Advanced Cells - DL; Dishes BCT |
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354601 | CORNING | 5/pk | EUR 330 |
Description: Advanced Cells - DL; Dishes BCT |
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354602 | CORNING | 10/pk | EUR 304.8 |
Description: Advanced Cells - DL; Dishes BCT |
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25-055-CM | Scientific Laboratory Supplies | PK6 | EUR 85.2 |
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25-055-CV | Scientific Laboratory Supplies | PK6 | EUR 58.8 |