Antibodies Assay Kits Atg7 Antibody Calmodulin Antibody cDNA Chat Antibody Clia Kits Devices DNA DNA Templates Eed Antibody Elisa Kits Enzymes Equipments Exosomes Gels Gli2 Antibody Igf1 Antibody Isotypes Medium & Serums Panel Particles PCR Pcr Kits Peptides Reagents Ria Kits Rip3 Antibody Satb2 Antibody Tfam Antibody Txnip Antibody Vhh Antibody
Live-cell imaging of microglial interactions with radial glia in transgenic embryonic mouse brains using slice culture
Microglial dynamics and interactions with close by radial glia may be visualized in actual time in embryonic mouse mind tissue utilizing time-lapse imaging in slice tradition. This live-cell imaging protocol can be utilized to review the morphology and actions of a variety of cell varieties throughout quite a lot of mind areas and developmental time factors. The benefit of this mind slice tradition mannequin is that it permits for the visualization of mobile interactions and actions in actual time, particularly throughout embryogenesis. For full particulars on the use and execution of this protocol, please check with Rosin et al. (2021).
Evaluation on the prokaryotic microbiome in females and embryonic cell cultures of Rhipicephalus sanguineus tropical and temperate lineages from two particular localities in Brazil
Two lineages of Rhipicephalus sanguineus are identified in Brazil: the temperate or southern and the tropical or northern populations. The distribution patterns of each lineages of R. sanguineus have epidemiological implications that may have an effect on vectorial competence regarding Ehrlichia canis, the agent of canine monocytic ehrlichiosis. Desiring to determine the microbiomes of each lineages and evaluate microorganisms in R. sanguineus, we used the 16S rRNA (V4-V5 area) gene-based metataxonomic method, by means of NGS sequencing on the MiSeq Illumina platform. We chosen specimens of females from the surroundings and samples of main embryonic cell cultures, from each lineages, and this was the primary research to analyze the prokaryotic microbiome in tick cell cultures. The outcomes confirmed that many bacterial taxa detected within the samples had been typical members of the host surroundings. A major variety of microorganisms in R. sanguineus females and in embryonic cell cultures from each lineages was discovered, with emphasis on the presence of Coxiella in all samples, albeit in several proportions. The Coxiella species current within the two lineages of ticks could also be completely different and should have co-evolved with them, thus driving completely different patterns of interactions between ticks and the pathogens that they’ll harbor or transmit to vertebrate hosts.
Human umbilical cord-derived mesenchymal stem cells have an effect on urea synthesis and the cell apoptosis of human induced hepatocytes by secreting IL-6 in a serum-free co-tradition system
Background: Bioartificial livers (BALs) are rising as a possible supportive remedy for liver illnesses. Nonetheless, the upkeep of hepatocyte operate and viability is a significant problem. Mesenchymal stem cells (MSCs) have attracted in depth consideration for offering trophic help to hepatocytes, however solely few research have explored the interplay between human MSCs and human hepatocytes, and little or no is thought in regards to the underlying molecular mechanisms whereby MSCs have an effect on hepatocyte operate, particularly in serum-free medium (SFM).
Conclusion: The SFM co-culture technique confirmed main benefits in sustaining hiHep operate and viability, which is of nice significance for the scientific software of hiHeps in BALs. This text is protected by copyright. All rights reserved.
Key phrases: BALs; Co-culture; IL-6; Serum-free medium; human MSCs; human-induced hepatocytes.
A protocol for analyzing the protein terminome of human most cancers cell line tradition supernatants
Characterization of protein termini is important for understanding how the proteome is generated by means of organic processes similar to post-translational proteolytic occasions. Right here, we introduce a sensible protocol for terminomics to attain easy and delicate N- and C-terminal peptide enrichment. We apply it to the terminome evaluation of tradition supernatants of a human most cancers cell line for the aim of figuring out ectodomain shedding substrate cleavage websites with 10 μg protein per pattern. For full particulars on the use and execution of this protocol, please check with Tsumagari et al. (2021).
Improvement of 3D cultures of zebrafish liver and embryo cell strains: a comparability of various spheroid formation strategies
Fish cell spheroids are promising 3D tradition fashions for vertebrate alternative in ecotoxicology. Nonetheless, new various ecotoxicological strategies should be tailored for purposes in business and for regulatory functions; such strategies should be cost-effective, easy to control and supply speedy outcomes. Due to this fact, we in contrast the effectiveness of the normal hanging drop (HD), orbital shaking (OS), and HD mixed with OS (HD+OS) strategies on the formation of zebrafish cell line spheroids (ZFL and ZEM2S). Time in HD (3-5 days) and completely different 96-well plates [flat-bottom or ultra-low attachment of round-bottom (ULA-plates)] in OS had been evaluated. Straightforward dealing with, speedy spheroid formation, uniform-sized spheroids, and circularity had been assessed to determine the very best spheroid protocol.
Conventional HD alone didn’t lead to ZFL spheroid formation, whereas HD (5 days)+OS did. When utilizing the OS, spheroids solely shaped on the ULA-plate. Each HD+OS and OS had been reproducible in measurement (177.50 ± 2.81 µm and 225.62 ± 19.20 µm, respectively) and circularity (0.83 ± 0.02 and 0.80 ± 0.01, respectively) of ZFL spheroids. Nonetheless, HD+OS required a substantial time to utterly type spheroids (10 days) and intensive dealing with, whereas the OS was quick (5 days of incubation) and easy.
OS additionally yielded reproducible ZEM2S spheroids in 1 day (226.23 ± 0.57 µm diameter and 0.80 ± 0.01 circularity). In conclusion, OS in ULA-plate is an efficient and easy spheroid protocol for high-throughput ecotoxicity testing. This research contributes to determine a quick, reproducible, and easy protocol of single piscine spheroid formation in 96-well plates and helps the applying of fish 3D mannequin in business and academia.
Immunomodulatory potential of Clinacanthus nutans extracts within the co-tradition of triple-negative breast most cancers cells, MDA-MB-231, and THP-1 macrophages
Triple-negative breast most cancers is the primary sort of breast carcinoma that causes mortality amongst ladies due to the restricted remedy choices and excessive recurrence. Power irritation has been linked with the tumor microenvironment (TME) in breast most cancers development.
Clinacanthus nutans (CN) has gained a lot consideration due to its anticancer properties, however its mechanism stays unclear. We aimed to review the qualitative phytochemical content material and elucidate the cytotoxicity results of CN on human triple-negative breast most cancers (TNBC), MDA-MB-231 and human macrophage-like cells similar to THP-1 by utilizing sulforhodamine B (SRB) assay. As extremely metastatic cells, MDA-MB-231 cells can migrate to the distal place, the impact of CN on migration had been additionally elucidated utilizing the scratch assay. The CN results on ameliorating continual irritation in TME had been studied following the co-culture of MDA-MB-231/THP-1 macrophages. The cytokine expression ranges of IL-6, IL-1β and tumor necrosis factor-alpha (TNF-α) had been decided utilizing ELISA assays.
The outcomes confirmed that each ethanolic and aqueous CN extracts contained alkaloid, phenol and tannin, flavonoid, terpenoid, glycoside and steroid. Nonetheless, saponin was solely discovered within the aqueous extract of CN. CN was not cytotoxic to each MDA-MB-231 and THP-1 cells. The flexibility of MDA-MB-231 emigrate was additionally not halted by CN remedy.
Nonetheless, CN ethanol extract decreased IL-6 at 25 μg/mL (p = 0.02) and 100 μg/mL (p = 0.03) however CN aqueous extract elevated IL-6 expression at 50 μg/mL (p = 0.08) and 100 μg/mL (p = 0.02). IL-1β confirmed decreased expression after handled with CN ethanol and CN aqueous each at 25 μg/mL (p = 0.03). TNF-α had been considerably decreased after CN ethanol remedy at focus 25- (p = 0.001), 50- (p = 0.000) and 100 μg/mL (p = 0.000). CN aqueous extract barely inhibited TNF-α in any respect 25-50- and 100 μg/mL (p = 0.001, p = 0.000, p = 0.000, respectively). General, CN acts by ameliorating the pro-inflammatory situation within the TME and could also be a possible technique for its anticancer mechanism on extremely metastatic breast most cancers situation. The main pathways that hyperlink each most cancers and irritation had been NF-κB and STATs thus additional research on the upstream and downstream pathways is required to completely perceive the mechanism of CN extracts in cooling the infected TME in breast most cancers.
 Bead Sample Pack - 5E Set |
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| BSP-5E | Next Advance | 1pack | EUR 302.4 |
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Description: Bead sample pack for Bullet Blender 5E models. Includes 10mL of: ZROB015, ZROB05, ZROB20, SSB02, SSB14B, SSB32, SSB48, SSUFO35 and SSUFO56. |
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 Bead Sample Pack - 5M Set |
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| BSP-5Y | Next Advance | 1pack | EUR 289.2 |
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Description: Bead sample pack for Bullet Blender 5mL Storm models. Includes 10mL of: ZROB015, ZROB05, ZROB20, SSB02, SSB14B, SSB32, SSB60, SSB110 and SSUFO35. |
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 Bead Sample Pack - Full Set |
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| BSP-ALL2 | Next Advance | 1pack | EUR 460.8 |
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Description: Bead sample pack inlcudes sampling of all 20 bead types in 10mL tubes. |
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 Bead Sample Pack - Microcentrifuge Set |
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| BSP-MC2 | Next Advance | 1pack | EUR 278.4 |
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Description: Bead sample pack for Bullet Blender 1.5mL tube models. Includes 10mL of: ZROB015, ZROB05, ZROB10, ZROB20, SSB02, SSB14B, SSB32 and SSUFO35. |
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 Bead Sample Pack - Organ Tissues |
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| BSP-OT3 | Next Advance | 1pack | EUR 282 |
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Description: Bead sample pack for homogenization of organ tissues. Includes 10mL of: ZROB05, ZROB10, ZROB20, SSB14B, SSB32 and SSUFO35. |
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 All Sample Diluents Sample Pack |
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| KF17355 | Neuromics | 3X100 ml | EUR 326.4 |
 Red Blood Cell Sample Pack |
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| 88R-1050 | Fitzgerald | 6 x 2 ml | EUR 483.6 |
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Description: Horse, Mouse, Rabbit, Sheep, Bovine and Chicken glutaraldehyde stabilized blood cell samples |
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 TMB Peroxidase Substrate SAMPLE PACK |
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| 42R-TB101 | Fitzgerald | 3 x 100 ml | EUR 360 |
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Description: 100mL of 3 different TMB Peroxidase Substrates ready to use in immunoassays: Ultra sensitivite, Highly Sensitive, and Slow Acting. |
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 3 Blocking Buffer Sample Pack |
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| KF17340 | Neuromics | 3 x 100 ml | EUR 246 |
 All Assay Diluents Sample Pack |
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| KF17351 | Neuromics | 4X100 ml | EUR 326.4 |
 GC Headspace Demo Sample Pack |
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| TNDPSSHS | Scientific Laboratory Supplies | EACH | EUR 3.77 |
 GC/HPLC Demo Sample Pack |
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| TNDPSSSV | Scientific Laboratory Supplies | EACH | EUR 3.77 |
 Water, 0.1um Filtered, cell cultures tested |
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| W0900-050 | GenDepot | 500 ml | EUR 81.6 |
Water, 0.1um Filtered, cell cultures tested |
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| W0900-310 | GenDepot | 10x500 ml | EUR 202.8 |
Water, 0.1um Filtered, cell cultures tested |
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| W0900-350 | GenDepot | 50x500 ml | EUR 627.6 |
 Micro Bead Sterlizer |
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| B1201-E | Benchmark Scientific | 1 PC | EUR 651.24 |
Micro Bead Sterlizer |
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| B1202-E | Benchmark Scientific | 1 PC | EUR 100.97 |
 Refill Glass Beads |
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| B1201-BEAD | Benchmark Scientific | 1 PC | EUR 141.34 |
 Column Packing Kit |
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| PACK-KIT | Next Advance | 1pack | EUR 1242 |
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Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
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 Sample Cell 10ml Matched |
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| 2495402 | Scientific Laboratory Supplies | PK2 | EUR 349.84 |
 Bangs Lab Bead Solution |
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| SOLN1-1000 | Bangs Laboratories | 1000 ML | EUR 186.07 |
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Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres |
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Bangs Lab Bead Solution |
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| SOLN1-2000 | Bangs Laboratories | 2000 ML | EUR 255.24 |
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Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres |
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Bangs Lab Bead Solution |
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| SOLN1-500 | Bangs Laboratories | 500 ML | EUR 118.21 |
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Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres |
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 Sample Diluent |
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| I094 | Cygnus Technologies | 1000 ml | EUR 622.8 |
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Description: Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
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Sample Diluent |
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| I094-100 | Cygnus Technologies | 100 ml | EUR 253.2 |
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Description: Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
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Sample Diluent |
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| I094-500 | Cygnus Technologies | 500 ml | EUR 447.6 |
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Description: Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
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 CM Rapid Run Agarose Bead |
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| CMRR-25 | ABTBeads | 25 ml | EUR 122.4 |
CM Rapid Run Agarose Bead |
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| CMRR-300 | ABTBeads | 300 ml | EUR 590.4 |
 DEAE Rapid Run Agarose Bead |
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| DEAERR-25 | ABTBeads | 25 ml | EUR 122.4 |
DEAE Rapid Run Agarose Bead |
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| DEAERR-300 | ABTBeads | 300 ml | EUR 590.4 |
 SP Rapid Run Agarose Bead |
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| SPRR-25 | ABTBeads | 25 ml | EUR 122.4 |
SP Rapid Run Agarose Bead |
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| SPRR-300 | ABTBeads | 300 ml | EUR 590.4 |
 Glass bead diameter 1 mm |
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| DD68499 | Scientific Laboratory Supplies | EACH | EUR 26.68 |
 Glass bead diameter 2 mm |
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| DD68500 | Scientific Laboratory Supplies | EACH | EUR 37.62 |
 Glass bead diameter 3mm 500g |
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| DD68501 | Scientific Laboratory Supplies | EACH | EUR 39.9 |
 Glass bead diameter 4 mm |
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| DD68502 | Scientific Laboratory Supplies | EACH | EUR 35.34 |
 Glass bead diameter 5 mm |
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| DD68503 | Scientific Laboratory Supplies | EACH | EUR 37.62 |
 Glass bead diameter 6 mm |
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| DD68504 | Scientific Laboratory Supplies | EACH | EUR 36.48 |
 Glass bead diameter 8 mm |
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| DD68505 | Scientific Laboratory Supplies | BAG | EUR 42.64 |
 Glass bead diameter 10 mm |
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| DD68555 | Scientific Laboratory Supplies | EACH | EUR 42.52 |
 Sample Cell 1 Inch Round Glass |
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| WAT1118 | Scientific Laboratory Supplies | PK6 | EUR 120.02 |
 Sample Cell 1 Inch Round PS |
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| WAT1120 | Scientific Laboratory Supplies | PK2 | EUR 26.81 |
 Adapter pack |
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| C1005-AC2 | Benchmark Scientific | 1 PC | EUR 118.2 |
Adapter pack |
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| C3100-ADP | Benchmark Scientific | 1 PC | EUR 197.02 |
 EZ Pack |
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| A2501 | Benchmark Scientific | 1 PC | EUR 438.79 |
EZ Pack |
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| A2505 | Benchmark Scientific | 1 PC | EUR 219.64 |
 Sanitisation pack |
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| WAT2432 | Scientific Laboratory Supplies | EACH | EUR 106.02 |
 Disinfection Pack |
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| WAT3344 | Scientific Laboratory Supplies | EACH | EUR 129.96 |
 Agarose bead Conjugated) Anti-DDK(FLAG) Agarose bead Conjugated |
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| D4501-001 | GenDepot | 1ml | EUR 660 |
Anti-DDK(FLAG) Agarose bead Conjugated |
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| D4501-005 | GenDepot | 5ml | EUR 1647.6 |
) RNA/DNA Purification Kit (Magnetic Bead) |
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| DA0623 | Daan Gene | 32 test/kit | Ask for price |
) Nickel NTA Magnetic Agarose Bead (5%) |
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| MagNTANi5-10 | ABTBeads | 10ml suspension | EUR 340.8 |
Nickel NTA Magnetic Agarose Bead (5%) |
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| MagNTANi5-2 | ABTBeads | 2ml suspension | EUR 118.8 |
Nickel NTA Magnetic Agarose Bead (5%) |
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| MagNTANi5-5 | ABTBeads | 5ml suspension | EUR 208.8 |
 DNA Sample Diluent |
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| D006-100 | Cygnus Technologies | 100 ml | EUR 447.6 |
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Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
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DNA Sample Diluent |
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| D006-1000 | Cygnus Technologies | 1000 ml | EUR 1400.4 |
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Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
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DNA Sample Diluent |
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| D006-500 | Cygnus Technologies | 500 ml | EUR 871.2 |
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Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
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 Rotavirus Positive Sample |
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| DAG-H10377 | Creative Diagnostics | 1 ml dry ice | EUR 1326 |
 RSV Positive Sample |
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| DAG-H10378 | Creative Diagnostics | 1 ml dry ice | EUR 1326 |
 BSA Sample Diluent |
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| F031A | Cygnus Technologies | 1000 ml | EUR 585.6 |
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Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
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BSA Sample Diluent |
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| F031A-100 | Cygnus Technologies | 100 ml | EUR 234 |
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Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
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BSA Sample Diluent |
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| F031A-500 | Cygnus Technologies | 500 ml | EUR 493.2 |
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Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
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 Sample Denaturing Buffer |
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| F054 | Cygnus Technologies | 12 ml | EUR 234 |
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Description: Sample Denaturing Buffer by Cygnus Technologies is available in Europe via Gentaur. |
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 Sample Treatment Buffer |
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| F175 | Cygnus Technologies | 1000 ml | EUR 493.2 |
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Description: Sample Treatment Buffer by Cygnus Technologies is available in Europe via Gentaur. |
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Sample Treatment Buffer |
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| F175-100 | Cygnus Technologies | 100 ml | EUR 262.8 |
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Description: Sample Treatment Buffer by Cygnus Technologies is available in Europe via Gentaur. |
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 Sample Treatment Plate |
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| F402 | Cygnus Technologies | 1x 96 wells plate | EUR 123.6 |
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Description: Sample Treatment Plate by Cygnus Technologies is available in Europe via Gentaur. |
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 ELISA Sample Diluent |
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| ESD125 | ScyTek Laboratories | 125 ml | EUR 96 |
ELISA Sample Diluent |
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| ESD500 | ScyTek Laboratories | 500 ml | EUR 130.8 |
