Live-cell imaging of microglial interactions with radial glia in transgenic embryonic mouse brains using slice culture
Evaluation on the prokaryotic microbiome in females and embryonic cell cultures of Rhipicephalus sanguineus tropical and temperate lineages from two particular localities in Brazil
Human umbilical cord-derived mesenchymal stem cells have an effect on urea synthesis and the cell apoptosis of human induced hepatocytes by secreting IL-6 in a serum-free co-tradition system
A protocol for analyzing the protein terminome of human most cancers cell line tradition supernatants
Improvement of 3D cultures of zebrafish liver and embryo cell strains: a comparability of various spheroid formation strategies
Fish cell spheroids are promising 3D tradition fashions for vertebrate alternative in ecotoxicology. Nonetheless, new various ecotoxicological strategies should be tailored for purposes in business and for regulatory functions; such strategies should be cost-effective, easy to control and supply speedy outcomes. Due to this fact, we in contrast the effectiveness of the normal hanging drop (HD), orbital shaking (OS), and HD mixed with OS (HD+OS) strategies on the formation of zebrafish cell line spheroids (ZFL and ZEM2S). Time in HD (3-5 days) and completely different 96-well plates [flat-bottom or ultra-low attachment of round-bottom (ULA-plates)] in OS had been evaluated. Straightforward dealing with, speedy spheroid formation, uniform-sized spheroids, and circularity had been assessed to determine the very best spheroid protocol.
Conventional HD alone didn’t lead to ZFL spheroid formation, whereas HD (5 days)+OS did. When utilizing the OS, spheroids solely shaped on the ULA-plate. Each HD+OS and OS had been reproducible in measurement (177.50 ± 2.81 µm and 225.62 ± 19.20 µm, respectively) and circularity (0.83 ± 0.02 and 0.80 ± 0.01, respectively) of ZFL spheroids. Nonetheless, HD+OS required a substantial time to utterly type spheroids (10 days) and intensive dealing with, whereas the OS was quick (5 days of incubation) and easy.
OS additionally yielded reproducible ZEM2S spheroids in 1 day (226.23 ± 0.57 µm diameter and 0.80 ± 0.01 circularity). In conclusion, OS in ULA-plate is an efficient and easy spheroid protocol for high-throughput ecotoxicity testing. This research contributes to determine a quick, reproducible, and easy protocol of single piscine spheroid formation in 96-well plates and helps the applying of fish 3D mannequin in business and academia.
Immunomodulatory potential of Clinacanthus nutans extracts within the co-tradition of triple-negative breast most cancers cells, MDA-MB-231, and THP-1 macrophages
Bead Sample Pack - 5M Set | |||
BSP-5Y | Next Advance | 1pack | EUR 289.2 |
Description: Bead sample pack for Bullet Blender 5mL Storm models. Includes 10mL of: ZROB015, ZROB05, ZROB20, SSB02, SSB14B, SSB32, SSB60, SSB110 and SSUFO35. |
Bead Sample Pack - 50mL Set | |||
BSP-50B2 | Next Advance | 1pack | EUR 484.8 |
Description: Bead sample pack for Bullet Blender 50mL tube models. Includes 20mL of: ZROB05, ZROB20, SSB02, SSB14B, SSB48, SSB60, SSUFO35 and SSUFO56. |
Bead Sample Pack - Full Set | |||
BSP-ALL2 | Next Advance | 1pack | EUR 460.8 |
Description: Bead sample pack inlcudes sampling of all 20 bead types in 10mL tubes. |
Bead Sample Pack - Organ Tissues | |||
BSP-OT3 | Next Advance | 1pack | EUR 282 |
Description: Bead sample pack for homogenization of organ tissues. Includes 10mL of: ZROB05, ZROB10, ZROB20, SSB14B, SSB32 and SSUFO35. |
Bead Sample Pack - Microcentrifuge Set | |||
BSP-MC2 | Next Advance | 1pack | EUR 278.4 |
Description: Bead sample pack for Bullet Blender 1.5mL tube models. Includes 10mL of: ZROB015, ZROB05, ZROB10, ZROB20, SSB02, SSB14B, SSB32 and SSUFO35. |
Water, 0.1um Filtered, cell cultures tested | |||
W0900-050 | GenDepot | 500 ml | EUR 81.6 |
Water, 0.1um Filtered, cell cultures tested | |||
W0900-310 | GenDepot | 10x500 ml | EUR 202.8 |
Water, 0.1um Filtered, cell cultures tested | |||
W0900-350 | GenDepot | 50x500 ml | EUR 627.6 |
Red Blood Cell Sample Pack | |||
88R-1050 | Fitzgerald | 6 x 2 ml | EUR 483.6 |
Description: Horse, Mouse, Rabbit, Sheep, Bovine and Chicken glutaraldehyde stabilized blood cell samples |
CULTURE VESSEL, C1770 SAMPLE | |||
C2099 | PhytoTechnology Laboratories | 10EA | EUR 10.56 |
All Sample Diluents Sample Pack | |||
KF17355 | Neuromics | 3X100 ml | EUR 326.4 |
PTcon SAMPLE PACK | |||
C2125 | PhytoTechnology Laboratories | 5EA | EUR 32.34 |
Growth Supplement for Anaerobic Cultures | |||
FD805-100G | EWC Diagnostics | 1 unit | EUR 16.61 |
Description: Growth Supplement for Anaerobic Cultures |
GELLING AGENT SAMPLE PACK | |||
G3500 | PhytoTechnology Laboratories | 1EA | EUR 17.71 |
3 Blocking Buffer Sample Pack | |||
KF17340 | Neuromics | 3 x 100 ml | EUR 246 |
All Assay Diluents Sample Pack | |||
KF17351 | Neuromics | 4X100 ml | EUR 326.4 |
96 Well Cell Culture Plate, White, Flat bottom, TC, Sterile, 1/pack, 100/cs | |||
701301 | NEST Biotechnology | 100 pcs/cs | EUR 103.73 |
Description: 96 Well Cell Culture Plate, White, Flat bottom, TC, Sterile, 1/pack, 100/cs |
TMB Peroxidase Substrate SAMPLE PACK | |||
42R-TB101 | Fitzgerald | 3 x 100 ml | EUR 393 |
Description: 100mL of 3 different TMB Peroxidase Substrates ready to use in immunoassays: Ultra sensitivite, Highly Sensitive, and Slow Acting. |
GC/HPLC Demo Sample Pack - EACH | |||
TNDPSSSV | Scientific Laboratory Supplies | EACH | EUR 5.4 |
Cell Culture Flask,3 Layer, Vent Cap, TC, Sterile,Bag Package, 1 pc/pack, 12 pak | |||
731302 | NEST Biotechnology | 12 pcs/cs | EUR 65.89 |
Description: Cell Culture Flask,3 Layer, Vent Cap, TC, Sterile,Bag Package, 1 pc/pack, 12 pakcs/case, 12 pcs/case |
GC Headspace Demo Sample Pack - EACH | |||
TNDPSSHS | Scientific Laboratory Supplies | EACH | EUR 5.4 |
Cell Culture Flask,3 Layer, Plug Seal Cap, TC, Sterile,Bag Package, 1 pc/pack, 1 | |||
731301 | NEST Biotechnology | 12 pcs/cs | EUR 64.9 |
Description: Cell Culture Flask,3 Layer, Plug Seal Cap, TC, Sterile,Bag Package, 1 pc/pack, 12 pakcs/case, 12 pcs/case |
S-adenosylmethionine ELISA kit for cell culture supernatant or other samples | |||
IK00204 | Arthus | 96 tests | EUR 699 |
Description: S-adenosylmethionine ELISA kit for cell culture supernatant or other samples |
S-adenosylhomocysteine ELISA kit for cell culture supernatant or other samples | |||
IK00304 | Arthus | 96 tests | EUR 1049 |
Description: S-adenosylhomocysteine ELISA kit for cell culture supernatant or other samples |
Supreme Plant Tissue Culture Grade Agar | |||
PCTA100 | Plant Cell Technology | 500g | EUR 144 |
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media. |
Supreme Plant Tissue Culture Grade Agar | |||
PCTA500 | Plant Cell Technology | 1000g | EUR 180 |
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media. |
CORNING® BIOCOAT™ POLY-D-LYSINE/LAMININ 8 WELL CULTURESLIDE, 4/PACK, 12/CASE | |||
354688 | CORNING | 3/pk | EUR 543.6 |
Description: Advanced Cells - DL; Slips/Slides |
96 Well Cell Culture Plate, White, Flat bottom, Non-Treated, Sterile, 1/pack, 10 | |||
701311 | NEST Biotechnology | 100 pcs/cs | EUR 101.75 |
Description: 96 Well Cell Culture Plate, White, Flat bottom, Non-Treated, Sterile, 1/pack, 100/cs |
Sample Diluent Optimization Pack: 100mL SD1, SD2, SD3, PFSD | |||
959 | Immunochemistry | pack | EUR 187 |
Multifuge X4 Pro cell culture package - EACH | |||
75016067 | Scientific Laboratory Supplies | EACH | EUR 18158.85 |
Trueprep® AUTO MTB Sample Pre-treatment Pack | |||
60204AS05 | Molbio Diagnostics Private Limited | 5T | EUR 5 |
Trueprep® AUTO MTB Sample Pre-treatment Pack | |||
60204AS20 | Molbio Diagnostics Private Limited | 20T | EUR 20 |
Trueprep® AUTO MTB Sample Pre-treatment Pack | |||
60205AS50 | Molbio Diagnostics Private Limited | 50T | EUR 50 |
Multifuge X4R Pro cell culture package - EACH | |||
75016066 | Scientific Laboratory Supplies | EACH | EUR 24460.65 |
Trueprep® AUTO Universal Sample Pre-treatment Pack | |||
60205AB05 | Molbio Diagnostics Private Limited | 5T | EUR 5 |
Trueprep® AUTO Universal Sample Pre-treatment Pack | |||
60205AB20 | Molbio Diagnostics Private Limited | 20T | EUR 20 |
Trueprep® AUTO Universal Sample Pre-treatment Pack | |||
60205AB50 | Molbio Diagnostics Private Limited | 50T | EUR 50 |
Human Collagen I Coated Culture Ware - T25 10flasks / pack | |||
cAP-5002-t25 | Angio Proteomie | 1Pack | EUR 198 |
Human Collagen I Coated Culture Ware - T75 10flasks / pack | |||
cAP-5002-t75 | Angio Proteomie | 1Pack | EUR 316.8 |
Human Fibronectin Coated Culture Ware - T25 10flasks/pack | |||
cAP-5001-t25 | Angio Proteomie | 1Pack | EUR 198 |
Human Fibronectin Coated Culture Ware - T75 10flasks/pack | |||
cAP-5001-t75 | Angio Proteomie | 1Pack | EUR 316.8 |
SL4R Plus Centrifuge Cell Culture TX1000 Package - EACH | |||
75016075 | Scientific Laboratory Supplies | EACH | EUR 20652.3 |
Human Collagen I Coated Culture Ware - 12 wells 5plates/ pack | |||
cAP-5002-w12 | Angio Proteomie | 1Pack | EUR 168.3 |
Human Collagen I Coated Culture Ware - 24 wells 5plates/ pack | |||
cAP-5002-w24 | Angio Proteomie | 1Pack | EUR 178.2 |
Human Collagen I Coated Culture Ware - 384 wells 5plates/ pack | |||
cAP-5002-w384 | Angio Proteomie | 1Pack | EUR 396 |
Human Collagen I Coated Culture Ware - 48 wells 5plates/ pack | |||
cAP-5002-w48 | Angio Proteomie | 1Pack | EUR 198 |
Human Collagen I Coated Culture Ware - 6 wells 5plates/ pack | |||
cAP-5002-w6 | Angio Proteomie | 1Pack | EUR 158.4 |
Human Collagen I Coated Culture Ware - 96 wells 5plates/ pack | |||
cAP-5002-w96 | Angio Proteomie | 1Pack | EUR 247.5 |
Human Fibronectin Coated Culture Ware - 12 wells 5 plates/pack | |||
cAP-5001-w12 | Angio Proteomie | 1Pack | EUR 163.35 |
Human Fibronectin Coated Culture Ware - 24 wells 5 plates/pack | |||
cAP-5001-w24 | Angio Proteomie | 1Pack | EUR 178.2 |
Human Fibronectin Coated Culture Ware - 384 wells 5 plates/pack | |||
cAP-5001-w384 | Angio Proteomie | 1Pack | EUR 396 |
Human Fibronectin Coated Culture Ware - 48 wells 5 plates/pack | |||
cAP-5001-w48 | Angio Proteomie | 1Pack | EUR 198 |
Human Fibronectin Coated Culture Ware - 6 wells 5 plates/pack | |||
cAP-5001-w6 | Angio Proteomie | 1Pack | EUR 158.4 |
Human Fibronectin Coated Culture Ware - 96 wells 5 plates/pack | |||
cAP-5001-w96 | Angio Proteomie | 1Pack | EUR 247.5 |
Corning 100mm TC-Treated Culture Dishes in 6-pack Carriers - PK480 | |||
430293 | Scientific Laboratory Supplies | PK480 | EUR 571.05 |
Protein A Tell-Tale Gold® Ribbon Sample Pack, 3 x (1 cm x 30 cm) | |||
TTGA-SP3-B090 | BioAssayWorks | 3 x (1 cm x 30 cm) | EUR 208.7 |
Protein G Tell-Tale Gold® Ribbon Sample Pack, 3 x (1 cm x 30 cm) | |||
TTGG-SP3-B090 | BioAssayWorks | 3 x (1 cm x 30 cm) | EUR 208.7 |
Total RNA Mini Kit (Blood/Cultured Cell) - Individual Packed Columns | |||
RB004 | Geneaid Biotech | each | EUR 3 |
Total RNA Mini Kit (Blood/Cultured Cell) - Individual Packed Columns | |||
RB050 | Geneaid Biotech | each | EUR 82.3 |
Total RNA Mini Kit (Blood/Cultured Cell) - Individual Packed Columns | |||
RB100 | Geneaid Biotech | each | EUR 150.7 |
Total RNA Mini Kit (Blood/Cultured Cell) - Individual Packed Columns | |||
RB300 | Geneaid Biotech | each | EUR 396.5 |
CORNING® BIOCOAT™ POLY-D-LYSINE 60MM CULTURE DISHES, 20/PACK, 100/CASE, NONSTERILE | |||
356468 | CORNING | 20/pk | EUR 469.2 |
Description: Advanced Cells - DL; Dishes BCT |
Protein A/G Tell-Tale Gold® Ribbon Sample Pack, 3 x (1 cm x 30 cm) | |||
TTGAG-SP3-B090 | BioAssayWorks | 3 x (1 cm x 30 cm) | EUR 208.7 |
Gryphon Ecotropic Packaging Cell Line for Retrovirus (1 x 106 cells, Culture) | |||
ABP-RVC-10002C | Allele Biotech | 1 flask | Ask for price |
Description: PRODUCT LINE DISCONTINUED. Please contact us. |
CORNING® BIOCOAT™ COLLAGEN I 35MM TC-TREATED CULTURE DISHES, 100/PACK, 100/CASE, NONSTERILE | |||
356456 | CORNING | 20/pk | EUR 469.2 |
Description: Advanced Cells - DL; Dishes BCT |
Platinum-E Retroviral Packaging Cell Line, Ecotropic | |||
RV-101 | Cell Biolabs | 1 vial | EUR 770 |
Platinum-GP Retroviral Packaging Cell Line, Pantropic | |||
RV-103 | Cell Biolabs | 1 vial | EUR 770 |
Gryphon Amphotropic Packaging Cell Line for Retrovirus (1 x 106 cells, Culture) | |||
ABP-RVC-10001C | Allele Biotech | 1 flask | Ask for price |
Description: PRODUCT LINE DISCONTINUED. Please contact us. |