
Live-cell imaging of microglial interactions with radial glia in transgenic embryonic mouse brains using slice culture
Evaluation on the prokaryotic microbiome in females and embryonic cell cultures of Rhipicephalus sanguineus tropical and temperate lineages from two particular localities in Brazil
Human umbilical cord-derived mesenchymal stem cells have an effect on urea synthesis and the cell apoptosis of human induced hepatocytes by secreting IL-6 in a serum-free co-tradition system
A protocol for analyzing the protein terminome of human most cancers cell line tradition supernatants
Improvement of 3D cultures of zebrafish liver and embryo cell strains: a comparability of various spheroid formation strategies
Fish cell spheroids are promising 3D tradition fashions for vertebrate alternative in ecotoxicology. Nonetheless, new various ecotoxicological strategies should be tailored for purposes in business and for regulatory functions; such strategies should be cost-effective, easy to control and supply speedy outcomes. Due to this fact, we in contrast the effectiveness of the normal hanging drop (HD), orbital shaking (OS), and HD mixed with OS (HD+OS) strategies on the formation of zebrafish cell line spheroids (ZFL and ZEM2S). Time in HD (3-5 days) and completely different 96-well plates [flat-bottom or ultra-low attachment of round-bottom (ULA-plates)] in OS had been evaluated. Straightforward dealing with, speedy spheroid formation, uniform-sized spheroids, and circularity had been assessed to determine the very best spheroid protocol.
Conventional HD alone didn’t lead to ZFL spheroid formation, whereas HD (5 days)+OS did. When utilizing the OS, spheroids solely shaped on the ULA-plate. Each HD+OS and OS had been reproducible in measurement (177.50 ± 2.81 µm and 225.62 ± 19.20 µm, respectively) and circularity (0.83 ± 0.02 and 0.80 ± 0.01, respectively) of ZFL spheroids. Nonetheless, HD+OS required a substantial time to utterly type spheroids (10 days) and intensive dealing with, whereas the OS was quick (5 days of incubation) and easy.
OS additionally yielded reproducible ZEM2S spheroids in 1 day (226.23 ± 0.57 µm diameter and 0.80 ± 0.01 circularity). In conclusion, OS in ULA-plate is an efficient and easy spheroid protocol for high-throughput ecotoxicity testing. This research contributes to determine a quick, reproducible, and easy protocol of single piscine spheroid formation in 96-well plates and helps the applying of fish 3D mannequin in business and academia.

Immunomodulatory potential of Clinacanthus nutans extracts within the co-tradition of triple-negative breast most cancers cells, MDA-MB-231, and THP-1 macrophages
![]() Bead Sample Pack - 5E Set |
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BSP-5E | Next Advance | 1pack | EUR 252 |
Description: Bead sample pack for Bullet Blender 5E models. Includes 10mL of: ZROB015, ZROB05, ZROB20, SSB02, SSB14B, SSB32, SSB48, SSUFO35 and SSUFO56. |
![]() Bead Sample Pack - 5M Set |
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BSP-5Y | Next Advance | 1pack | EUR 241 |
Description: Bead sample pack for Bullet Blender 5mL Storm models. Includes 10mL of: ZROB015, ZROB05, ZROB20, SSB02, SSB14B, SSB32, SSB60, SSB110 and SSUFO35. |
![]() Bead Sample Pack - Full Set |
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BSP-ALL2 | Next Advance | 1pack | EUR 384 |
Description: Bead sample pack inlcudes sampling of all 20 bead types in 10mL tubes. |
![]() Bead Sample Pack - Microcentrifuge Set |
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BSP-MC2 | Next Advance | 1pack | EUR 232 |
Description: Bead sample pack for Bullet Blender 1.5mL tube models. Includes 10mL of: ZROB015, ZROB05, ZROB10, ZROB20, SSB02, SSB14B, SSB32 and SSUFO35. |
![]() Bead Sample Pack - Organ Tissues |
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BSP-OT3 | Next Advance | 1pack | EUR 235 |
Description: Bead sample pack for homogenization of organ tissues. Includes 10mL of: ZROB05, ZROB10, ZROB20, SSB14B, SSB32 and SSUFO35. |
![]() All Sample Diluents Sample Pack |
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KF17355 | Neuromics | 3X100 ml | EUR 272 |
![]() Red Blood Cell Sample Pack |
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88R-1050 | Fitzgerald | 6 x 2 ml | EUR 403 |
Description: Horse, Mouse, Rabbit, Sheep, Bovine and Chicken glutaraldehyde stabilized blood cell samples |
![]() Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample |
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P100EZ-sample | 101Bio | - | Ask for price |
![]() 3 Blocking Buffer Sample Pack |
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KF17340 | Neuromics | 3 x 100 ml | EUR 205 |
![]() All Assay Diluents Sample Pack |
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KF17351 | Neuromics | 4X100 ml | EUR 272 |
![]() TMB Peroxidase Substrate SAMPLE PACK |
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42R-TB101 | Fitzgerald | 3 x 100 ml | EUR 300 |
Description: 100mL of 3 different TMB Peroxidase Substrates ready to use in immunoassays: Ultra sensitivite, Highly Sensitive, and Slow Acting. |
![]() Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample |
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P101EZ--sample | 101Bio | - | Ask for price |
![]() Water, 0.1um Filtered, cell cultures tested |
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W0900-050 | GenDepot | 500 ml | EUR 68 |
![]() Water, 0.1um Filtered, cell cultures tested |
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W0900-310 | GenDepot | 10x500 ml | EUR 169 |
![]() Water, 0.1um Filtered, cell cultures tested |
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W0900-350 | GenDepot | 50x500 ml | EUR 523 |
![]() Micro Bead Sterlizer |
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B1201-E | Benchmark Scientific | 1 PC | EUR 542.7 |
![]() Micro Bead Sterlizer |
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B1202-E | Benchmark Scientific | 1 PC | EUR 84.14 |
![]() Column Packing Kit |
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PACK-KIT | Next Advance | 1pack | EUR 1035 |
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
![]() Sample Diluent |
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I094 | Cygnus Technologies | 1000 ml | EUR 519 |
Description: Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
![]() Sample Diluent |
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I094-100 | Cygnus Technologies | 100 ml | EUR 211 |
Description: Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
![]() Sample Diluent |
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I094-500 | Cygnus Technologies | 500 ml | EUR 373 |
Description: Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
![]() Refill Glass Beads |
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B1201-BEAD | Benchmark Scientific | 1 PC | EUR 117.78 |
![]() Bangs Lab Bead Solution |
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SOLN1-1000 | Bangs Laboratories | 1000 ML | EUR 155.06 |
Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres |
![]() Bangs Lab Bead Solution |
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SOLN1-2000 | Bangs Laboratories | 2000 ML | EUR 212.7 |
Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres |
![]() Bangs Lab Bead Solution |
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SOLN1-500 | Bangs Laboratories | 500 ML | EUR 98.51 |
Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres |
![]() EZ Pack |
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A2501 | Benchmark Scientific | 1 PC | EUR 365.66 |
![]() EZ Pack |
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A2505 | Benchmark Scientific | 1 PC | EUR 183.03 |
![]() Adapter pack |
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C1005-AC2 | Benchmark Scientific | 1 PC | EUR 98.5 |
![]() Adapter pack |
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C3100-ADP | Benchmark Scientific | 1 PC | EUR 164.18 |
![]() Long-term Cell Tracer 580 (Green) sample |
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P710GS | 101Bio | - | Ask for price |
![]() Long-term Cell Tracer 580 (Yellow) sample |
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P710YS | 101Bio | - | Ask for price |
![]() Sample Diluent Buffer |
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I028-100 | Cygnus Technologies | 100 ml | EUR 211 |
Description: Sample Diluent Buffer by Cygnus Technologies is available in Europe via Gentaur. |
![]() Sample Diluent Buffer |
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I028-1000 | Cygnus Technologies | 1000 ml | EUR 519 |
Description: Sample Diluent Buffer by Cygnus Technologies is available in Europe via Gentaur. |
![]() Sample Diluent Buffer |
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I028-500 | Cygnus Technologies | 500 ml | EUR 373 |
Description: Sample Diluent Buffer by Cygnus Technologies is available in Europe via Gentaur. |
![]() HEPES Sample Diluent |
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I050 | Cygnus Technologies | 1000 ml | EUR 527 |
Description: HEPES Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
![]() HEPES Sample Diluent |
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I050-100 | Cygnus Technologies | 100 ml | EUR 219 |
Description: HEPES Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
![]() HEPES Sample Diluent |
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I050-500 | Cygnus Technologies | 500 ml | EUR 334 |
Description: HEPES Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
![]() Sample Treatment Buffer |
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F175 | Cygnus Technologies | 1000 ml | EUR 411 |
Description: Sample Treatment Buffer by Cygnus Technologies is available in Europe via Gentaur. |
![]() Sample Treatment Buffer |
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F175-100 | Cygnus Technologies | 100 ml | EUR 219 |
Description: Sample Treatment Buffer by Cygnus Technologies is available in Europe via Gentaur. |
![]() Sample Treatment Plate |
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F402 | Cygnus Technologies | 1x 96 wells plate | EUR 103 |
Description: Sample Treatment Plate by Cygnus Technologies is available in Europe via Gentaur. |
![]() BSA Sample Diluent |
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F031A | Cygnus Technologies | 1000 ml | EUR 488 |
Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
![]() BSA Sample Diluent |
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F031A-100 | Cygnus Technologies | 100 ml | EUR 195 |
Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
![]() BSA Sample Diluent |
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F031A-500 | Cygnus Technologies | 500 ml | EUR 411 |
Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
![]() Sample Denaturing Buffer |
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F054 | Cygnus Technologies | 12 ml | EUR 195 |
Description: Sample Denaturing Buffer by Cygnus Technologies is available in Europe via Gentaur. |
![]() DNA Sample Diluent |
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D006-100 | Cygnus Technologies | 100 ml | EUR 373 |
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
![]() DNA Sample Diluent |
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D006-1000 | Cygnus Technologies | 1000 ml | EUR 1167 |
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
![]() DNA Sample Diluent |
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D006-500 | Cygnus Technologies | 500 ml | EUR 726 |
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
![]() Rotavirus Positive Sample |
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DAG-H10377 | Creative Diagnostics | 1 ml dry ice | EUR 1105 |
![]() RSV Positive Sample |
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DAG-H10378 | Creative Diagnostics | 1 ml dry ice | EUR 1105 |
![]() Sample diluent buffer |
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AR1106-1 | BosterBio | 30ml | EUR 91 |
![]() Sample Diluent Buffer |
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abx296001-20ml | Abbexa | 20 ml | EUR 91 |
![]() Sample Diluent, 60ML |
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X028-60ML | Arbor Assays | 60ML | EUR 143 |
![]() ELISA Sample Diluent |
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ESD125 | ScyTek Laboratories | 125 ml | EUR 80 |
![]() ELISA Sample Diluent |
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ESD500 | ScyTek Laboratories | 500 ml | EUR 109 |
![]() ELISA Sample Diluent |
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ESD999 | ScyTek Laboratories | 1000 ml | EUR 157 |
![]() Protein A Tell-Tale Gold Ribbon Sample Pack, 3 x (1 cm x 30 cm) |
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TTGA-SP3-B090 | BioAssayWorks | 3 x (1 cm x 30 cm) | EUR 227.67 |
Description: The pack contains polyester ribbon, which is soaked with 0.13 O.D./cm2 concentration with Protein G conjugated to gold nanoparticles. |
![]() Protein G Tell-Tale Gold Ribbon Sample Pack, 3 x (1 cm x 30 cm) |
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TTGG-SP3-B090 | BioAssayWorks | 3 x (1 cm x 30 cm) | EUR 227.67 |
Description: The pack contains polyester ribbon, which is soaked with 3 different concentrations with Protein G conjugated to gold nanoparticles. |
![]() CM Rapid Run Agarose Bead |
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CMRR-25 | ABTBeads | 25 ml | EUR 102 |
![]() CM Rapid Run Agarose Bead |
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CMRR-300 | ABTBeads | 300 ml | EUR 492 |
![]() DEAE Rapid Run Agarose Bead |
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DEAERR-25 | ABTBeads | 25 ml | EUR 102 |
![]() DEAE Rapid Run Agarose Bead |
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DEAERR-300 | ABTBeads | 300 ml | EUR 492 |
![]() SP Rapid Run Agarose Bead |
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SPRR-25 | ABTBeads | 25 ml | EUR 102 |
![]() SP Rapid Run Agarose Bead |
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SPRR-300 | ABTBeads | 300 ml | EUR 492 |
![]() Protein A/G Tell-Tale Gold Ribbon Sample Pack, 3 x (1 cm x 30 cm) |
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TTGAG-SP3-B090 | BioAssayWorks | 3 x (1 cm x 30 cm) | EUR 227.67 |
Description: The pack contains polyester ribbon, which is soaked with 3 different concentrations with Protein A and G conjugated to gold nanoparticles. |
![]() mini cooling pack |
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EVS1100-COOL | Consort | ea | EUR 48 |
![]() maxi cooling pack |
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EVS1300-COOL | Consort | ea | EUR 82 |
![]() Spatulas (Large Pack) |
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SPAT1 | Next Advance | 50pack | EUR 301 |
Description: Individually wrapped spatulas. RNase/DNase free, non-pyrogenic, antistatic, sterile, plastic. Pack of 50. |
![]() Spatulas (Small Pack) |
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SPAT2 | Next Advance | 10pack | EUR 133 |
Description: Individually wrapped spatulas. RNase/DNase free, non-pyrogenic, antistatic, sterile, plastic. Pack of 10. |