
Live-cell imaging of microglial interactions with radial glia in transgenic embryonic mouse brains using slice culture
Evaluation on the prokaryotic microbiome in females and embryonic cell cultures of Rhipicephalus sanguineus tropical and temperate lineages from two particular localities in Brazil
Human umbilical cord-derived mesenchymal stem cells have an effect on urea synthesis and the cell apoptosis of human induced hepatocytes by secreting IL-6 in a serum-free co-tradition system
A protocol for analyzing the protein terminome of human most cancers cell line tradition supernatants
Improvement of 3D cultures of zebrafish liver and embryo cell strains: a comparability of various spheroid formation strategies
Fish cell spheroids are promising 3D tradition fashions for vertebrate alternative in ecotoxicology. Nonetheless, new various ecotoxicological strategies should be tailored for purposes in business and for regulatory functions; such strategies should be cost-effective, easy to control and supply speedy outcomes. Due to this fact, we in contrast the effectiveness of the normal hanging drop (HD), orbital shaking (OS), and HD mixed with OS (HD+OS) strategies on the formation of zebrafish cell line spheroids (ZFL and ZEM2S). Time in HD (3-5 days) and completely different 96-well plates [flat-bottom or ultra-low attachment of round-bottom (ULA-plates)] in OS had been evaluated. Straightforward dealing with, speedy spheroid formation, uniform-sized spheroids, and circularity had been assessed to determine the very best spheroid protocol.
Conventional HD alone didn’t lead to ZFL spheroid formation, whereas HD (5 days)+OS did. When utilizing the OS, spheroids solely shaped on the ULA-plate. Each HD+OS and OS had been reproducible in measurement (177.50 ± 2.81 µm and 225.62 ± 19.20 µm, respectively) and circularity (0.83 ± 0.02 and 0.80 ± 0.01, respectively) of ZFL spheroids. Nonetheless, HD+OS required a substantial time to utterly type spheroids (10 days) and intensive dealing with, whereas the OS was quick (5 days of incubation) and easy.
OS additionally yielded reproducible ZEM2S spheroids in 1 day (226.23 ± 0.57 µm diameter and 0.80 ± 0.01 circularity). In conclusion, OS in ULA-plate is an efficient and easy spheroid protocol for high-throughput ecotoxicity testing. This research contributes to determine a quick, reproducible, and easy protocol of single piscine spheroid formation in 96-well plates and helps the applying of fish 3D mannequin in business and academia.

Immunomodulatory potential of Clinacanthus nutans extracts within the co-tradition of triple-negative breast most cancers cells, MDA-MB-231, and THP-1 macrophages
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BSP-5E | Next Advance | 1pack | EUR 302.4 |
Description: Bead sample pack for Bullet Blender 5E models. Includes 10mL of: ZROB015, ZROB05, ZROB20, SSB02, SSB14B, SSB32, SSB48, SSUFO35 and SSUFO56. |
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BSP-5Y | Next Advance | 1pack | EUR 289.2 |
Description: Bead sample pack for Bullet Blender 5mL Storm models. Includes 10mL of: ZROB015, ZROB05, ZROB20, SSB02, SSB14B, SSB32, SSB60, SSB110 and SSUFO35. |
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BSP-ALL2 | Next Advance | 1pack | EUR 460.8 |
Description: Bead sample pack inlcudes sampling of all 20 bead types in 10mL tubes. |
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BSP-MC2 | Next Advance | 1pack | EUR 278.4 |
Description: Bead sample pack for Bullet Blender 1.5mL tube models. Includes 10mL of: ZROB015, ZROB05, ZROB10, ZROB20, SSB02, SSB14B, SSB32 and SSUFO35. |
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BSP-OT3 | Next Advance | 1pack | EUR 282 |
Description: Bead sample pack for homogenization of organ tissues. Includes 10mL of: ZROB05, ZROB10, ZROB20, SSB14B, SSB32 and SSUFO35. |
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KF17355 | Neuromics | 3X100 ml | EUR 326.4 |
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88R-1050 | Fitzgerald | 6 x 2 ml | EUR 483.6 |
Description: Horse, Mouse, Rabbit, Sheep, Bovine and Chicken glutaraldehyde stabilized blood cell samples |
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42R-TB101 | Fitzgerald | 3 x 100 ml | EUR 360 |
Description: 100mL of 3 different TMB Peroxidase Substrates ready to use in immunoassays: Ultra sensitivite, Highly Sensitive, and Slow Acting. |
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KF17340 | Neuromics | 3 x 100 ml | EUR 246 |
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KF17351 | Neuromics | 4X100 ml | EUR 326.4 |
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TNDPSSHS | Scientific Laboratory Supplies | EACH | EUR 3.77 |
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TNDPSSSV | Scientific Laboratory Supplies | EACH | EUR 3.77 |
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W0900-050 | GenDepot | 500 ml | EUR 81.6 |
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W0900-310 | GenDepot | 10x500 ml | EUR 202.8 |
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W0900-350 | GenDepot | 50x500 ml | EUR 627.6 |
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B1201-E | Benchmark Scientific | 1 PC | EUR 651.24 |
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2495402 | Scientific Laboratory Supplies | PK2 | EUR 349.84 |
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SOLN1-1000 | Bangs Laboratories | 1000 ML | EUR 186.07 |
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SOLN1-2000 | Bangs Laboratories | 2000 ML | EUR 255.24 |
Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres |
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SOLN1-500 | Bangs Laboratories | 500 ML | EUR 118.21 |
Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres |
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I094 | Cygnus Technologies | 1000 ml | EUR 622.8 |
Description: Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
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I094-100 | Cygnus Technologies | 100 ml | EUR 253.2 |
Description: Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
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I094-500 | Cygnus Technologies | 500 ml | EUR 447.6 |
Description: Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
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CMRR-25 | ABTBeads | 25 ml | EUR 122.4 |
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CMRR-300 | ABTBeads | 300 ml | EUR 590.4 |
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DEAERR-25 | ABTBeads | 25 ml | EUR 122.4 |
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SPRR-25 | ABTBeads | 25 ml | EUR 122.4 |
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SPRR-300 | ABTBeads | 300 ml | EUR 590.4 |
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DD68499 | Scientific Laboratory Supplies | EACH | EUR 26.68 |
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DD68503 | Scientific Laboratory Supplies | EACH | EUR 37.62 |
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DD68555 | Scientific Laboratory Supplies | EACH | EUR 42.52 |
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C1005-AC2 | Benchmark Scientific | 1 PC | EUR 118.2 |
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D4501-001 | GenDepot | 1ml | EUR 660 |
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D4501-005 | GenDepot | 5ml | EUR 1647.6 |
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DA0623 | Daan Gene | 32 test/kit | Ask for price |
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D006-100 | Cygnus Technologies | 100 ml | EUR 447.6 |
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D006-1000 | Cygnus Technologies | 1000 ml | EUR 1400.4 |
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
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DAG-H10377 | Creative Diagnostics | 1 ml dry ice | EUR 1326 |
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DAG-H10378 | Creative Diagnostics | 1 ml dry ice | EUR 1326 |
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F031A | Cygnus Technologies | 1000 ml | EUR 585.6 |
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F031A-100 | Cygnus Technologies | 100 ml | EUR 234 |
Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
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F175-100 | Cygnus Technologies | 100 ml | EUR 262.8 |
Description: Sample Treatment Buffer by Cygnus Technologies is available in Europe via Gentaur. |
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F402 | Cygnus Technologies | 1x 96 wells plate | EUR 123.6 |
Description: Sample Treatment Plate by Cygnus Technologies is available in Europe via Gentaur. |
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ESD125 | ScyTek Laboratories | 125 ml | EUR 96 |
![]() ELISA Sample Diluent |
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ESD500 | ScyTek Laboratories | 500 ml | EUR 130.8 |