Category: Calmodulin Antibody
Electrochemical measurement of serotonin by Au-CNT electrodes fabricated on microporous cell culture membranes
Intestine-brain axis (GBA) communication depends on serotonin (5-HT) signaling between the intestine epithelium and the peripheral nervous system, the place 5-HT launch patterns from the basolateral (i.e., backside) aspect of the epithelium activate nerve afferents. There have been few quantitative research of this gut-neuron signaling because of an absence of real-time measurement instruments that may…
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Live-cell imaging of microglial interactions with radial glia in transgenic embryonic mouse brains using slice culture
Microglial dynamics and interactions with close by radial glia may be visualized in actual time in embryonic mouse mind tissue utilizing time-lapse imaging in slice tradition. This live-cell imaging protocol can be utilized to review the morphology and actions of a variety of cell varieties throughout quite a lot of mind areas and developmental time…
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Characterization of Endogenous and Extruded H 2 S and Small Oxoacids of Sulfur (SOS) in Cell Cultures
This report characterizes and quantifies endogenous hydrogen sulfide (H2S) and small oxoacids of sulfur (SOS = HOSH, HOSOH) in a panel of cell traces together with human most cancers (A375 melanoma cells, HeLa cervical cells) and noncancer (HEK293 embryonic kidney cells), in addition to E. coli DH5α and S. cerevisiae S288C. The methodology used is a translation of well-studied…
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Differentiation of Human Pluripotent Stem Cells Into Definitive Endoderm Cells in Various Flexible Three-Dimensional Cell Culture Systems: Possibilities and Limitations
The era of human stem cell-derived spheroids and organoids represents a serious step in fixing quite a few medical, pharmacological, and organic challenges. Because of the benefits of three-dimensional (3D) cell tradition programs and the varied functions of human pluripotent stem cell (iPSC)-derived definitive endoderm (DE), we studied the affect of spheroid dimension and 3D…
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A digital microfluidic system with 3D microstructures for single-cell culture
Regardless of the exact controllability of droplet samples in digital microfluidic (DMF) methods, their functionality in isolating single cells for long-time tradition remains to be restricted: usually, just a few cells could be captured on an electrode. Though fabricating small-sized hydrophilic micropatches on an electrode aids single-cell seize, the actuation voltage for droplet transportation needs…
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Stable Long-Term Culture of Human Distal Airway Stem Cells for Transplantation
There’s a inhabitants of p63+/Krt5+ distal airway stem cells (DASCs) quiescently situated within the airway basal epithelium of mammals, responding to harm and airway epithelial regeneration. They maintain the flexibility to distinguish into a number of pulmonary cell varieties and may repopulate the epithelium after harm. The present examine goals at gaining additional insights into the…
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