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Differentiation of Human Pluripotent Stem Cells Into Definitive Endoderm Cells in Various Flexible Three-Dimensional Cell Culture Systems: Possibilities and Limitations
The era of human stem cell-derived spheroids and organoids represents a serious step in fixing quite a few medical, pharmacological, and organic challenges. Because of the benefits of three-dimensional (3D) cell tradition programs and the varied functions of human pluripotent stem cell (iPSC)-derived definitive endoderm (DE), we studied the affect of spheroid dimension and 3D cell tradition programs on spheroid morphology and the effectiveness of DE differentiation as assessed by quantitative PCR (qPCR), movement cytometry, immunofluorescence, and computational modeling.
Among the many examined hydrogel-based 3D programs, we discovered that basement membrane extract (BME) hydrogel couldn’t retain spheroid morphology because of dominant cell-matrix interactions. Then again, we discovered that nanofibrillar cellulose (NFC) hydrogel may preserve spheroid morphology however impeded progress issue diffusion, thereby negatively affecting cell differentiation. In distinction, suspension tradition supplied ample mass switch and was demonstrated by protein expression assays, morphological analyses, and mathematical modeling to be superior to the hydrogel-based programs.
As well as, we discovered that spheroid dimension was reversely correlated with the effectiveness of DE formation. Nonetheless, spheroids of inadequate sizes did not retain 3D morphology throughout differentiation in all of the studied tradition situations. We hereby display how the properties of a selected biomaterial affect the differentiation course of and the significance of spheroid dimension management for profitable human iPSC differentiation. Our research offers important parametric info for the era of human DE-derived, tissue-specific organoids in future research.
Biotechnological manufacturing of diterpenoid lactones from cell and organ cultures of Andrographis paniculata
Andrographis paniculata (AP) is a medicinal plant that’s historically utilized in Indian, Chinese language, Malay, Thai, and Oriental system of medicines to deal with numerous issues. AP consists of andrographolide (AD), 14-deoxy-11,12-didehydroandrographolide (DDAD), and neoandrographolide (NAD) as main diterpene lactones which has extraordinarily bitter properties; subsequently, AP is usually known as “King of bitters.” AD, DDAD, and NAD are reported to own therapeutic values similar to antioxidant, immunostimulatory, hepatoprotective, anti-cancer, anti-inflammatory, anti-rheumatoidal, anti-malarial, anti-leishmanial, anti-fertility, anti-obesity, antipyretic, and antimicrobial attributes.
In line with the Indian Pharmacopoeia, the leaves and tender shoots of AP yield as much as 1%, 0.16%, and 0.11% of AD, DDAD, and NAD, respectively, on a dry-weight foundation. Nonetheless, variability within the accumulation of AD, DDAD, and NAD in crops has been reported with respect to species, genotype, season, phenological stage, plant half used, and geography of a area of cultivation.
Due to this fact, cell and tissue tradition programs particularly cell, shoot, and adventitious root cultures are explored as alternate options for fixed and better manufacturing of AD, DDAD, and NAD. This overview explores the prospects of exploiting the plant cell and tissue tradition programs for the managed manufacturing of AD, DDAD, and NAD. Varied methods similar to elicitation by utilizing organic and chemical elicitors are explored for the enhancement of accumulation of AD, DDAD, and NAD in cell and organ cultures.
KEY POINTS: • This overview explores the probabilities of diterpene lactone manufacturing from cell and organ cultures. • Varied methods are explored for the improved accumulation of AD, DDAD, and NAD in cell and organ cultures. • Prospects of diterpene lactone manufacturing are highlighted.
Metabolomics evaluation of the 3D L-02 cell cultures revealing the important thing function of metabolism of amino acids in ameliorating hepatotoxicity of perfluorooctanoic acid
To simulate the true cell standing and morphology within the residing programs is substantial for utilizing cell fashions to handle the detrimental results of poisonous contaminants. On this research, the comparative profiles of metabolites in three-dimensional (3D) human regular liver (L-02) cell spheroids with perfluorooctanoic acid (PFOA) remedy had been analyzed utilizing a metabolomic method. The uniform 3D cell spheroids had been nicely fashioned in three days (e.g., sphericity index >0.9) and stably maintained over the following 11 days. The cytotoxicity of PFOA to the 3D L-02 cell spheroids was extremely depending on each publicity focus and period. Comparative evaluation of metabolomes confirmed that the variety of differential metabolites within the 3D cell spheroids handled with 300 μM PFOA for 10 days (n = 59) was higher than these with a 4-day publicity to 300 μM PFOA (n = 17). Six metabolic pathways associated to amino acids metabolism had been solely discovered within the 3D cell spheroids with a 10-day remedy of 300 μM PFOA, which couldn’t be discovered within the 2D monolayer cells and people 3D cell spheroids with a 4-day publicity. The suppression of PFOA on glutamine metabolism considerably decreased glutathione (GSH) manufacturing and accordingly elevated the extent of reactive oxygen species within the 3D cell spheroids. Quite the opposite, the supplementation of glutamine elevated GSH manufacturing and the viability of cell spheroids, indicating that glutamine metabolism performed a important function within the power poisonous results of PFOA. Our research strongly urged that complete toxicological methodologies primarily based on the 3D cell fashions may at present be sturdy and appropriate for addressing the power opposed results of poisonous contaminants.

Extremely self-healable and injectable cellulose hydrogels through fast hydrazone linkage for drug supply and 3D cell tradition
To fulfill the rising demand of injectable hydrogels with self-healing, robustness and biocompatibility for biomedical engineering, the reversible ketoester-type acylhydrazone linkages was used for the fabrication of novel cellulose-based hydrogel. The ketoester-type acylhydrazone bond exchanged quickly, endowing the hydrogels with extremely environment friendly self-healing efficiency with none exterior stimuli below physiological atmosphere, which was hardly achieved with the broadly used arylhydrozone bond.
The dynamic hydrogels exhibited tunable mechanical property, pH responsiveness, injectability and biocompatibility, demonstrating immense functions prospect for numerous biomedicines, similar to drug and cell supply. The pH-responsive managed launch of mannequin drug doxorubicin (DOX) loaded within the hydrogel was demonstrated.
As well as, benefitting from the superb biocompatibility and the reversible ketoester-type acylhydrazone bonds, cells had been encapsulated within the hydrogels as 3D service. The covalent adaptable community intensified injectability of cell-laden hydrogels and improved the long-lasting viability for cell tradition, displaying nice potential within the biomedical subject.
Multifunctional Thermoresponsive Microcarriers for Excessive-Throughput Cell Tradition and Enzyme-Free Cell Harvesting
An efficient remedy of human ailments utilizing regenerative drugs and cell remedy approaches requires a lot of cells. Cultivation of cells on microcarriers is a promising method because of the excessive surface-to-volume ratios that these microcarriers provide. Right here, multifunctional temperature-responsive microcarriers (cytoGel) manufactured from an interpenetrating hydrogel community composed of poly(N-isopropylacrylamide) (PNIPAM), poly(ethylene glycol) diacrylate (PEGDA), and gelatin methacryloyl (GelMA) are developed. A flow-focusing microfluidic chip is used to supply microcarriers with diameters within the vary of 100-300 μm and uniform dimension distribution (polydispersity index of ≈0.08).
The mechanical properties and cells adhesion properties of cytoGel are adjusted by altering the composition hydrogel composition. Notably, GelMA regulates the temperature response and enhances microcarrier stiffness. Human-derived glioma cells (U87) are grown on cytoGel in static and dynamic tradition situations with cell viabilities higher than 90%. Enzyme-free cell detachment is achieved at room temperature with as much as 70% detachment effectivity. Managed launch of bioactive molecules from cytoGel is completed for over per week to showcase the potential use of microcarriers for localized supply of progress elements to cell surfaces. These microcarriers maintain nice promise for the environment friendly growth of cells for the industrial-scale tradition of therapeutic cells.
![]() BME 100X Vitamins for Basal Medium Eagle (Modified) |
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BML01-100ML | Caisson Labs | 100 ml | EUR 87.6 |
Description: 100X Vitamins for Basal Medium Eagle (Modified) |
![]() BME 100X Vitamins for Basal Medium Eagle (Modified) |
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BML01-500ML | Caisson Labs | 500 ml | EUR 110.4 |
Description: 100X Vitamins for Basal Medium Eagle (Modified) |
![]() Gamborg's B-5 Medium |
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CP009-010 | GenDepot | 10X1L | EUR 118.8 |
![]() Gamborg's B-5 Medium |
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CP009-500 | GenDepot | 50L | EUR 151.2 |
![]() Gamborg's B-5 Long Medium |
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CP013-010 | GenDepot | 10X1L | EUR 135.6 |
![]() Gamborg's B-5 Long Medium |
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CP013-100 | GenDepot | 10L | EUR 121.2 |
![]() Gamborg's B-5 Medium; With 20g/L Sucrose |
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CP010-010 | GenDepot | 10X1L | EUR 124.8 |
![]() Gamborg's B-5 Medium; With 20g/L Sucrose |
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CP010-500 | GenDepot | 50L | EUR 141.6 |
![]() MURASHIGE SKOOG AGAR W/ VITAMINS |
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M13-148-10kg | Alphabiosciences | 10 kg | Ask for price |
![]() MURASHIGE SKOOG AGAR W/ VITAMINS |
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M13-148-2kg | Alphabiosciences | 2kg | Ask for price |
![]() MURASHIGE SKOOG AGAR W/ VITAMINS |
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M13-148-500g | Alphabiosciences | 500 g | Ask for price |
![]() Gamborg's Vitamin Stcks, Liquid |
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CP049-010 | GenDepot | 125 ml bottle | EUR 128.4 |
![]() 100mL MEM Vitamins 100x Solution |
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25-020-CI | Scientific Laboratory Supplies | PK6 | EUR 115.2 |
![]() Murashige and Skoog, With Vitamins |
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CP030-010 | GenDepot | 10X1L | EUR 118.8 |
![]() Murashige and Skoog, With Vitamins |
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CP030-500 | GenDepot | 50L | EUR 151.2 |
![]() Murashige and Skoog, With Vitamins and Glycine |
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CP031-010 | GenDepot | 10X1L | EUR 118.8 |
![]() Murashige and Skoog, With Vitamins and Glycine |
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CP031-500 | GenDepot | 50L | EUR 151.2 |
![]() DC MEDIUM W/ BCIG |
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D04-116-10kg | Alphabiosciences | 10 kg | EUR 5446.8 |
![]() DC MEDIUM W/ BCIG |
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D04-116-2Kg | Alphabiosciences | 2 Kg | EUR 1231.2 |
![]() DC MEDIUM W/ BCIG |
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D04-116-500g | Alphabiosciences | 500 g | EUR 378 |
![]() Morpheus III - 15% Vitamins mix, 50ml *SHIPPED ON ICE* |
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M-MD2-50-314 | MiTeGen | each | EUR 250 |
Description: Morpheus III - 15% Vitamins mix, 50ml *SHIPPED ON ICE* |
![]() Murashige and Skoog, With Vitamins, Glycine, and 30g/L Sucrose |
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CP032-010 | GenDepot | 10X1L | EUR 121.2 |
![]() Murashige and Skoog, With Vitamins, Glycine, and 30g/L Sucrose |
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CP032-500 | GenDepot | 50L | EUR 151.2 |
![]() Schneider's Medium, w/ L-glutamine |
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CCM1318-500 | Bio Basic | 500 mL | EUR 104.33 |
![]() 500mL MEM Alpha Medium w salt |
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15-012-CV | Scientific Laboratory Supplies | PK6 | EUR 193.2 |
![]() 500mL MEM Alpha Medium w salts |
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10-022-CV | Scientific Laboratory Supplies | PK6 | EUR 243.6 |
![]() Vitamin B5 [HRP] |
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DAGA-123H | Creative Diagnostics | 1mg | EUR 1466.4 |
![]() Vitamin B5 [KLH] |
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DAGA-123K | Creative Diagnostics | 1mg | EUR 1482 |
![]() Medium 199, with L-Glutamine, w/ Earle's salts |
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CCM2441-500 | Bio Basic | 500 mL | EUR 81.68 |
![]() Human Vitamin B5(VB5) |
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QY-E05699 | Qayee Biotechnology | 96T | EUR 433.2 |
![]() Medium 199 |
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C0012-01 | Addexbio | RT 500 mL Bottle | EUR 126 |
![]() Tyvek 500 Xpert Classic disposable Coverall c/w hood Medium |
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SAF4086 | Scientific Laboratory Supplies | PK100 | EUR 1280.4 |
![]() A Medium |
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DJ1018 | Bio Basic | 100g | EUR 101.76 |
![]() DC MEDIUM |
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D04-117-10kg | Alphabiosciences | 10 kg | EUR 1894.8 |
![]() DC MEDIUM |
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D04-117-2kg | Alphabiosciences | 2kg | EUR 458.4 |
![]() DC MEDIUM |
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D04-117-500g | Alphabiosciences | 500 g | EUR 168 |
![]() EC MEDIUM |
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E05-100-10kg | Alphabiosciences | 10 kg | EUR 976.8 |
![]() EC MEDIUM |
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E05-100-2Kg | Alphabiosciences | 2 Kg | EUR 259.2 |
![]() EC MEDIUM |
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E05-100-500g | Alphabiosciences | 500 g | EUR 114 |
![]() OF Medium |
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MED1664 | Scientific Laboratory Supplies | 500G | EUR 79.2 |
![]() M9 Medium |
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SD7024 | Bio Basic | 250g | EUR 86.1 |
![]() YM Medium |
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SD7031 | Bio Basic | 250g | EUR 84.54 |
![]() HLP MEDIUM |
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H08-107-10kg | Alphabiosciences | 10 kg | EUR 2733.6 |
![]() HLP MEDIUM |
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H08-107-2Kg | Alphabiosciences | 2 Kg | EUR 640.8 |
![]() HLP MEDIUM |
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H08-107-500g | Alphabiosciences | 500 g | EUR 218.4 |
![]() M63 Medium |
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SD7033 | Bio Basic | 250g | EUR 86.1 |
![]() SIM MEDIUM |
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S19-110-10kg | Alphabiosciences | 10 kg | EUR 1225.2 |
![]() SIM MEDIUM |
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S19-110-2kg | Alphabiosciences | 2kg | EUR 313.2 |
![]() SIM MEDIUM |
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S19-110-500g | Alphabiosciences | 500 g | EUR 128.4 |
![]() SOB MEDIUM |
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S19-124-10kg | Alphabiosciences | 10 kg | EUR 1158 |
![]() SOB MEDIUM |
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S19-124-2kg | Alphabiosciences | 2kg | EUR 298.8 |
![]() SOB MEDIUM |
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S19-124-500g | Alphabiosciences | 500 g | EUR 124.8 |
![]() TCBS Medium |
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MED1188 | Scientific Laboratory Supplies | PK10 | EUR 9.6 |
![]() M9CA Medium |
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SD7025 | Bio Basic | 250g | EUR 86.1 |
![]() DNase Medium |
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MED1196 | Scientific Laboratory Supplies | PK10 | EUR 10.8 |
![]() TYGPN Medium |
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SD7032 | Bio Basic | 250g | EUR 84.01 |
![]() Heller's Medium |
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CP014-010 | GenDepot | 10X1L | EUR 118.8 |
![]() Heller's Medium |
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CP014-500 | GenDepot | 50L | EUR 151.2 |
![]() Ec Medium 500gm |
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231430 | Scientific Laboratory Supplies | EACH | EUR 176.4 |
![]() Sob Medium 500gm |
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244310 | Scientific Laboratory Supplies | EACH | EUR 261.6 |
![]() Mounting Medium |
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4300 | Virostat | 3 ml | EUR 216.6 |
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein. |
![]() Advanced Medium |
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C0003-04 | Addexbio | RT 500 mL Bottle | EUR 123.6 |
![]() COLIFORM MEDIUM |
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C03-127-10kg | Alphabiosciences | 10 kg | EUR 1588.8 |
![]() COLIFORM MEDIUM |
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C03-127-2kg | Alphabiosciences | 2kg | EUR 392.4 |
![]() COLIFORM MEDIUM |
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C03-127-500g | Alphabiosciences | 500 g | EUR 150 |
![]() Hoagland's Medium |
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CP015-010 | GenDepot | 10X1L | EUR 118.8 |
![]() Hoagland's Medium |
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CP015-500 | GenDepot | 50L | EUR 151.2 |
![]() D-Pantothenic acid hemicalcium salt (Vitamin B5) |
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PB0683 | Bio Basic | 100g | EUR 82.97 |
![]() Insect Cell Medium: TNM-FH Insect Culture Medium |
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ABP-MED-10001 | Allele Biotech | 1 liter | Ask for price |
![]() Insect Cell Medium: Serum-Free Insect Culture Medium |
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ABP-MED-10002 | Allele Biotech | 1 liter | Ask for price |
![]() RPMI 1640 Medium |
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abx098879-500ml | Abbexa | 500 ml | EUR 210 |
![]() A-1 MEDIUM |
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A01-116-10kg | Alphabiosciences | 10 kg | EUR 1240.8 |
![]() A-1 MEDIUM |
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A01-116-2Kg | Alphabiosciences | 2 Kg | EUR 316.8 |
![]() A-1 MEDIUM |
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A01-116-500g | Alphabiosciences | 500 g | EUR 129.6 |
![]() MCDB 131 Medium |
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CM034-050 | GenDepot | 500ml | EUR 102 |
![]() MCDB 131 Medium |
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CM034-300 | GenDepot | 6x500ml | EUR 198 |
![]() MCDB 131 Medium |
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CM034-310 | GenDepot | 10x500ml | EUR 262.8 |
![]() MCDB 131 Medium |
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CM034-320 | GenDepot | 20x500ml | EUR 408 |
![]() MCDB 131 Medium |
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CM034-350 | GenDepot | 50x500ml | EUR 702 |
![]() Insect Cell Medium: Grace?s Insect Medium (Unsupplemented) |
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ABP-MED-10004 | Allele Biotech | 1 liter | Ask for price |
![]() BPS Medium 1 |
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79259 | BPS Bioscience | 100 ml | EUR 120 |
Description: This liquid BPS Medium is optimized for use with many BPS Reporter Kits including_x000D_ • 60612: AP1 Reporter Kit (JNK Pathway)_x000D_ • 60514: ARE Reporter Kit (Nrf2 Antioxidant Pathway)_x000D_ • 60611: CRE/CREB Reporter Kit (cAMP/PKA Signaling Pathway)_x000D_ • 60614: NF-κB Reporter Kit (NF-κB Signaling Pathway)_x000D_ • 60509: Notch1/CSL Reporter Kit (Notch Signaling Pathway)_x000D_ • 60654: SBE Reporter Kit (TGFβ/SMAD signaling pathway)_x000D_ • 60511: SRE Reporter Kit (MAPK/ERK Signaling Pathway)_x000D_ • 60500: TCF/LEF Reporter Kit (Wnt Signaling Pathway) |
![]() BPS Medium 7 |
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79260 | BPS Bioscience | 100 ml | EUR 120 |
Description: This liquid BPS Medium is optimized for use with select BPS reporter kits. |
![]() BPS Medium 8 |
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79385 | BPS Bioscience | 100 ml | EUR 120 |
Description: This liquid BPS Medium is optimized for use with the CSF1R/SRE Reporter Kit (BPS Bioscience, #79379). |
![]() NeuroProgenitor Medium |
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NM42400 | Neuromics | 125 ml | EUR 364.8 |
![]() Thaw Medium 4 |
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60181-1 | BPS Bioscience | 100 ml | EUR 140 |
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• CFTR - HEK 293 Recombinant Cell Line #60506• Nav1.8/β2 - HEK293 Recombinant Cell Line #60521• NaV1.7-HEK293 Recombinant Cell Line #60607 |
![]() Thaw Medium 4 |
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60181-2 | BPS Bioscience | 500 ml | EUR 600 |
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• CFTR - HEK 293 Recombinant Cell Line #60506• Nav1.8/β2 - HEK293 Recombinant Cell Line #60521• NaV1.7-HEK293 Recombinant Cell Line #60607 |
![]() Thaw Medium 5 |
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60182-1 | BPS Bioscience | 100 ml | EUR 140 |
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Gli Reporter - NIH3T3 Recombinant Cell line (Hedgehog Pathway) #60409 |
![]() Thaw Medium 5 |
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60182-2 | BPS Bioscience | 500 ml | EUR 600 |
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Gli Reporter - NIH3T3 Recombinant Cell line (Hedgehog Pathway) #60409 |
![]() Thaw Medium 6 |
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60183-1 | BPS Bioscience | 100 ml | EUR 140 |
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway) #60520• NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line #60623 • RARα Reporter - HEK293 Recombinant Cell Line #60503 • RARβ Reporter - HEK293 Recombinant Cell Line #60603 • RARγ Reporter - HEK293 Reporter Cell Line #60604 |
![]() Thaw Medium 6 |
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60183-2 | BPS Bioscience | 500 ml | EUR 600 |
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway) #60520• NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line #60623 • RARα Reporter - HEK293 Recombinant Cell Line #60503 • RARβ Reporter - HEK293 Recombinant Cell Line #60603 • RARγ Reporter - HEK293 Reporter Cell Line #60604 |
![]() Thaw Medium 2 |
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60184-1 | BPS Bioscience | 100 ml | EUR 150 |
Description: This liquid Cell Thawing Medium is optimized for thawing and plating BPS Bioscience's Jurkat-based cell lines, including• PD-1/NFAT Reporter-Jurkat cell #60535• NFAT Reporter (Luc) - Jurkat Cell Line #60621• NF-κB-Luciferase Reporter (Luc) - Jurkat Cell Line #60651• ADCC Bioassay Effector Cell F variant (Low Affinity) #60540• ADCC Bioassay Effector Cell V variant (High Affinity) #60541Note: once cells are well-established, switch to the appropriate cell growth medium. |
![]() Thaw Medium 2 |
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60184-2 | BPS Bioscience | 500 ml | EUR 675 |
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including_x000D__x000D_• PD-1/NFAT Reporter-Jurkat cell #60535_x000D__x000D_• NFAT Reporter (Luc) - Jurkat Cell Line #60621_x000D__x000D_• NF-ĸB-Luciferase Reporter (Luc) - Jurkat Cell Line #60651_x000D__x000D_• ADCC Bioassay Effector Cell F variant (Low Affinity) #60540_x000D__x000D_• ADCC Bioassay Effector Cell V variant (High Affinity) #60541_x000D__x000D_Note: once cells are well-established, switch to the appropriate cell growth medium. |
![]() Thaw Medium 7 |
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60185-1 | BPS Bioscience | 100 ml | EUR 140 |
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway) #60520 • NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line #60623 |
![]() Thaw Medium 7 |
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60185-2 | BPS Bioscience | 500 ml | EUR 600 |
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines, including • Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway) #60520 • NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line #60623 |
![]() Thaw Medium 3 |
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60186-1 | BPS Bioscience | 100 ml | EUR 150 |
Description: This liquid Cell Thaw Medium is optimized for thawing and plating BPS Bioscience's CHO cell-based recombinant cell lines, including• TCR Activator - CHO Recombinant Cell line #60539 • TCR activator / PD-L1 - CHO Recombinant Cell line #60536 It is designed for use in a 37°C incubator with an atmosphere of 5% CO2/95% air. Note: once cells are well established, switch to an appropriate cell growth medium. |
![]() Thaw Medium 3 |
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60186-2 | BPS Bioscience | 500 ml | EUR 645 |
Description: This liquid thaw medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines, including_x000D__x000D_• TCR Activator - CHO Recombinant Cell line #60539 _x000D_• TCR activator / PD-L1 - CHO Recombinant Cell line #60536 _x000D__x000D_• CD27 CHO-K1 Stable Recombiant Cell Line #60624 _x000D__x000D_• PD-L1 - CHO Recombinant Cell Line #60543 _x000D__x000D_• NF-κB Reporter (Luc) - CHO-K1 Recombinant Cell Line #60622_x000D_• CD137L (4-1BBL) CHO-K1 Recombinant Cell Line #60523_x000D__x000D_It is designed for use in a 37°C incubator with an atmosphere of 5% CO2/95% air. _x000D_Note: once cells are well established, switch to an appropriate cell growth medium._x000D_ |
![]() Thaw Medium 1 |
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60187-1 | BPS Bioscience | 100 ml | EUR 155 |
Description: This liquid HEK293 Cell Thawing Medium is optimized for thawing and plating BPS Bioscience's HEK293 recombinant cell lines. |
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