cellexusinc

A digital microfluidic system with 3D microstructures for single-cell culture

Regardless of the exact controllability of droplet samples in digital microfluidic (DMF) methods, their functionality in isolating single cells for long-time tradition remains to be restricted: usually, just a few cells could be captured on an electrode. Though fabricating small-sized hydrophilic micropatches on an electrode aids single-cell seize, the actuation voltage for droplet transportation needs to be considerably raised, leading to a shorter lifetime for the DMF chip and a bigger danger of damaging the cells.
On this work, a DMF system with 3D microstructures engineered on-chip is proposed to kind semi-closed micro-wells for environment friendly single-cell isolation and long-time tradition. Our optimum outcomes confirmed that roughly 20% of the micro-wells over a 30 × 30 array have been occupied by remoted single cells. As well as, low-evaporation-temperature oil and surfactant aided the system in reaching a low droplet actuation voltage of 36V, which was four instances decrease than the standard 150 V, minimizing the potential harm to the cells within the droplets and to the DMF chip. To exemplify the technological advances, drug sensitivity exams have been run in our DMF system to research the cell response of breast most cancers cells (MDA-MB-231) and breast regular cells (MCF-10A) to a broadly used chemotherapeutic drug, Cisplatin (Cis). The outcomes on-chip have been according to these screened in typical 96-well plates. This novel, easy and sturdy single-cell trapping technique has nice potential in organic analysis on the single cell degree.

3D Poly(Lactic Acid) Scaffolds Promote Totally different Behaviors on Endothelial Progenitors and Adipose-Derived Stromal Cells in Comparability With Commonplace 2D Cultures

Tissue engineering is a department of regenerative medication, which contains the mixture of biomaterials, cells and different bioactive molecules to regenerate tissues. Biomaterial scaffolds act as substrate and as bodily help for cells they usually also can reproduce the extracellular matrix cues. Though tissue engineering purposes in mobile remedy are likely to give attention to the usage of specialised cells from explicit tissues or stem cells, little consideration has been paid to endothelial progenitors, an vital cell kind in tissue regeneration.
We mixed 3D printed poly(lactic acid) scaffolds comprising two totally different pore sizes with human adipose-derived stromal cells (hASCs) and expanded CD133+ cells to judge how these two cell varieties reply to the totally different architectures. hASCs signify a really perfect supply of cells for tissue engineering purposes because of their low immunogenicity, paracrine exercise and talent to distinguish.
Expanded CD133+ cells have been remoted from umbilical twine blood and signify a supply of endothelial-like cells with angiogenic potential. Fluorescence microscopy and scanning electron microscopy confirmed that each cell varieties have been in a position to adhere to the scaffolds and preserve their attribute morphologies.
The porous PLA scaffolds stimulated cell cycle development of hASCs however led to an arrest within the G1 part and diminished proliferation of expanded CD133+ cells. Additionally, whereas hASCs maintained their undifferentiated profile after 7 days of tradition on the scaffolds, expanded CD133+ cells introduced a discount of the von Willebrand issue (vWF), which affected the cells’ angiogenic potential.
We didn’t observe adjustments in cell habits for any of the parameters analyzed between the scaffolds with totally different pore sizes, however the 3D atmosphere created by the scaffolds had totally different results on the cell varieties examined. In contrast to the extensively used mesenchymal stem cell varieties, the 3D PLA scaffolds led to reverse behaviors of the expanded CD133+ cells by way of cytotoxicity, proliferation and immunophenotype. The outcomes obtained reinforce the significance of finding out how totally different cell varieties reply to 3D tradition methods when contemplating the scaffold method for tissue engineering.

Cytotoxic and anti inflammatory results of chitosan and hemostatic gelatin in oral cell tradition

Chitosan is a biopolymer with bactericidal/bacteriostatic impact, biocompatible and biodegradable. It has been utilized in tissue engineering to exchange tissues partially or fully by releasing bioactive supplies or influencing cell progress, often in regenerative medication and dentistry. The purpose of this research was to judge the cytotoxic and anti inflammatory impact of chitosan alone or with hemostatic gelatin (Spongostand®) in cultures of human pulp cells (HPC), human gingival fibroblasts (HGF) and mouse pre-osteoblasts (MC3T3-E1, ATCC). HPC and HGF have been remoted from sufferers.
Cells have been subcultured in DMEM. Chitosan was inoculated at totally different concentrations (0-0.5%) and hemostatic gelatins impregnated with chitosan (0.19%) have been positioned instantly within the presence of cells and incubated for 24 hours. Cell viability was decided by MTT technique and imply cytotoxic focus (CC50) was calculated from the dose-response curve. Anti-inflammatory impact was calculated from the in vitro gingivitis mannequin induced with interleukin 1beta (IL-1β) in HGF and protein detection.
The information have been subjected to Shapiro-Wilk, Kruskal-Wallis and Mann-Whitney exams. Experiments have been carried out in triplicate of three impartial assays. Cell viability of HPC, HGF and MC3T3-E1 in touch with chitosan decreased considerably (p<0.05). The HPC have been probably the most delicate (CC50= 0.18%), adopted by HGF (CC50= 0.18%) and MC3T3-E1 (CC50= 0.19%).
The cytotoxicity of gelatins impregnated with chitosan decreased cell viability of HGF and HPC by 11% and 5%, respectively. The proinflammatory impact was diminished considerably within the gingivitis mannequin. To conclude, chitosan induces reasonable cytotoxic results alone or with hemostatic gelatin at 0.19%, in dose-dependent method, with anti-inflammatory results on human gingival fibroblasts. Using chitosan as a biomaterial could be a superb selection to be used in regenerative dentistry.
cellexusinc
cellexusinc

Cytotoxic and anti inflammatory results of chitosan and hemostatic gelatin in oral cell tradition

Chitosan is a biopolymer with bactericidal/bacteriostatic impact, biocompatible and biodegradable. It has been utilized in tissue engineering to exchange tissues partially or fully by releasing bioactive supplies or influencing cell progress, often in regenerative medication and dentistry. The purpose of this research was to judge the cytotoxic and anti inflammatory impact of chitosan alone or with hemostatic gelatin (Spongostand®) in cultures of human pulp cells (HPC), human gingival fibroblasts (HGF) and mouse pre-osteoblasts (MC3T3-E1, ATCC). HPC and HGF have been remoted from sufferers. Cells have been subcultured in DMEM.
Chitosan was inoculated at totally different concentrations (0-0.5%) and hemostatic gelatins impregnated with chitosan (0.19%) have been positioned instantly within the presence of cells and incubated for 24 hours. Cell viability was decided by MTT technique and imply cytotoxic focus (CC50) was calculated from the dose-response curve. Anti-inflammatory impact was calculated from the in vitro gingivitis mannequin induced with interleukin 1beta (IL-1β) in HGF and protein detection. The information have been subjected to Shapiro-Wilk, Kruskal-Wallis and Mann-Whitney exams. Experiments have been carried out in triplicate of three impartial assays. Cell viability of HPC, HGF and MC3T3-E1 in touch with chitosan decreased considerably (p<0.05). The HPC have been probably the most delicate (CC50= 0.18%), adopted by HGF (CC50= 0.18%) and MC3T3-E1 (CC50= 0.19%). The cytotoxicity of gelatins impregnated with chitosan decreased cell viability of HGF and HPC by 11% and 5%, respectively. The proinflammatory impact was diminished considerably within the gingivitis mannequin. To conclude, chitosan induces reasonable cytotoxic results alone or with hemostatic gelatin at 0.19%, in dose-dependent method, with anti-inflammatory results on human gingival fibroblasts. Using chitosan as a biomaterial could be a superb selection to be used in regenerative dentistry.

A Medium

DJ1018 100g
EUR 101.76

DC MEDIUM

D04-117-10kg 10 kg
EUR 1894.8

DC MEDIUM

D04-117-2kg 2kg
EUR 458.4

DC MEDIUM

D04-117-500g 500 g
EUR 168

Advanced Medium

C0003-04 RT 500 mL Bottle
EUR 123.6

Medium 199

C0012-01 RT 500 mL Bottle
EUR 126

COLIFORM MEDIUM

C03-127-10kg 10 kg
EUR 1588.8

COLIFORM MEDIUM

C03-127-2kg 2kg
EUR 392.4

COLIFORM MEDIUM

C03-127-500g 500 g
EUR 150

Heller's Medium

CP014-010 10X1L
EUR 118.8

Heller's Medium

CP014-500 50L
EUR 151.2

Hoagland's Medium

CP015-010 10X1L
EUR 118.8

Hoagland's Medium

CP015-500 50L
EUR 151.2

EC MEDIUM

E05-100-10kg 10 kg
EUR 976.8

EC MEDIUM

E05-100-2Kg 2 Kg
EUR 259.2

EC MEDIUM

E05-100-500g 500 g
EUR 114

Mounting Medium

4300 3 ml
EUR 216.6
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein.

NeuroProgenitor Medium

NM42400 125 ml
EUR 364.8

HLP MEDIUM

H08-107-10kg 10 kg
EUR 2733.6

HLP MEDIUM

H08-107-2Kg 2 Kg
EUR 640.8

HLP MEDIUM

H08-107-500g 500 g
EUR 218.4

SIM MEDIUM

S19-110-10kg 10 kg
EUR 1225.2

SIM MEDIUM

S19-110-2kg 2kg
EUR 313.2

SIM MEDIUM

S19-110-500g 500 g
EUR 128.4

SOB MEDIUM

S19-124-10kg 10 kg
EUR 1158

SOB MEDIUM

S19-124-2kg 2kg
EUR 298.8

SOB MEDIUM

S19-124-500g 500 g
EUR 124.8

M9 Medium

SD7024 250g
EUR 86.1

M9CA Medium

SD7025 250g
EUR 86.1

YM Medium

SD7031 250g
EUR 84.54

TYGPN Medium

SD7032 250g
EUR 84.01

M63 Medium

SD7033 250g
EUR 86.1

TCBS Medium

MED1188 PK10
EUR 7.52

DNase Medium

MED1196 PK10
EUR 7.98

OF Medium

MED1664 500G
EUR 68.4

Insect Cell Medium: TNM-FH Insect Culture Medium

ABP-MED-10001 1 liter Ask for price

Insect Cell Medium: Serum-Free Insect Culture Medium

ABP-MED-10002 1 liter Ask for price

Insect Cell Medium: Grace?s Insect Medium (Unsupplemented)

ABP-MED-10004 1 liter Ask for price

50ml TC Tubes, Conical, 440 units/box

04-5540150 440 units/box
EUR 85.2

GMP IL4, 50µg

04-GMP-HU-IL4-50UG 50µg
EUR 579.6
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.

SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE

04-GSB
  • EUR 144.00
  • EUR 96.00
  • 1L
  • 500mL
Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You!

rHu IL 2 , 3MIU

04-RHIL2-02F01 1 vial
EUR 298.8
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

rHu IL 2 , 3MIU , Lot 200908F02

04-RHIL2-08F02 1 vial
EUR 298.8
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

PRE-GMP rHu GM-CSF, Molgramostim-Leukoma

04-RHUGM-CSF-7A10 300 µg
EUR 462
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.

Monkeypox Virus Real Time PCR Kit

ZD-0076-01 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit

ZD-0076-02 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

Mouse Anti TNP Immunotoxicity

198-TNPG-1 100 µL
EUR 469.2

Exo-Check Exosome Antibody Array

322-EXO-FBS-50A-1 100 µg
EUR 469.2

1-Step Polymorphs, Human Cell Separation

71-AN221725 1
EUR 238.8

Rye Agar A

765-M1854-500G 500 g
EUR 82

Rye Agar B

765-M1855-500G 500 g
EUR 93

Malachite Green Phosphate Assay kit

65-POMG-25H 2500 tests
EUR 333

QuantiChrom Hemoglobin Assay Kit

65-DIHB-250 250 tests
EUR 473

Porcine Parvovirus Antibody Elisa Test Kit

767-LSY-30009 192 Wells/kit
EUR 382

Angiotensin III (Ang III) Antibody

20-abx101595
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

MCDB 131 Medium

CM034-050 500ml
EUR 102

MCDB 131 Medium

CM034-300 6x500ml
EUR 198

MCDB 131 Medium

CM034-310 10x500ml
EUR 262.8

MCDB 131 Medium

CM034-320 20x500ml
EUR 408

MCDB 131 Medium

CM034-350 50x500ml
EUR 702

Williams' Medium E

CM063-050 500ml
EUR 109.2

Williams' Medium E

CM063-300 6x500ml
EUR 319.2

Williams' Medium E

CM063-310 10x500ml
EUR 375.6

Williams' Medium E

CM063-320 20x500ml
EUR 724.8

Williams' Medium E

CM063-350 50x500ml
EUR 1170

H Medium Broth

DJ1019 100g
EUR 73.06

AddexBio Cryopreservation Medium

C0002-20mL RT vial
EUR 124.8

CARY & BLAIR MEDIUM

C03-131-10kg 10 kg
EUR 2622

CARY & BLAIR MEDIUM

C03-131-2Kg 2 Kg
EUR 616.8

CARY & BLAIR MEDIUM

C03-131-500g 500 g
EUR 211.2

Cereal Crops Medium

CP003-005 5L
EUR 135.6

Cereal Crops Medium

CP003-010 10X1L
EUR 202.8

Kao Michayluk Medium

CP017-010 10X1L
EUR 118.8

Kao Michayluk Medium

CP017-500 50L
EUR 151.2

Orchid Maintenance Medium

CP037-010 10X1L
EUR 130.8

Orchid Maintenance Medium

CP037-500 50L
EUR 165.6

Woody Plants Medium

CP040-010 10X1L
EUR 121.2

Woody Plants Medium

CP040-500 50L
EUR 151.2

EC MEDIUM, MODIFIED

E05-108-10kg 10 kg
EUR 1029.6

EC MEDIUM, MODIFIED

E05-108-2Kg 2 Kg
EUR 271.2

EC MEDIUM, MODIFIED

E05-108-500g 500 g
EUR 117.6

FLUID THIOGLYCOLLATE MEDIUM

F06-101-10kg 10 kg
EUR 950.4

FLUID THIOGLYCOLLATE MEDIUM

F06-101-2kg 2kg
EUR 253.2

FLUID THIOGLYCOLLATE MEDIUM

F06-101-500g 500 g
EUR 112.8

FluoreGuard Mounting Medium

FMM030 30 ml
EUR 115.2

FluoreGuard Mounting Medium

FMM060 60 ml
EUR 139.2

FluoreGuard Mounting Medium

FMM500 500 ml
EUR 392.4

FluoreGuard Mounting Medium

FMM999 1000 ml
EUR 636

Recovery Medium - 6x12mL

1711 4/PK
EUR 153.6

SuperMOUNT? Mounting Medium

1211-20
EUR 241.2