cellexusinc

A digital microfluidic system with 3D microstructures for single-cell culture

Regardless of the exact controllability of droplet samples in digital microfluidic (DMF) methods, their functionality in isolating single cells for long-time tradition remains to be restricted: usually, just a few cells could be captured on an electrode. Though fabricating small-sized hydrophilic micropatches on an electrode aids single-cell seize, the actuation voltage for droplet transportation needs to be considerably raised, leading to a shorter lifetime for the DMF chip and a bigger danger of damaging the cells.
On this work, a DMF system with 3D microstructures engineered on-chip is proposed to kind semi-closed micro-wells for environment friendly single-cell isolation and long-time tradition. Our optimum outcomes confirmed that roughly 20% of the micro-wells over a 30 × 30 array have been occupied by remoted single cells. As well as, low-evaporation-temperature oil and surfactant aided the system in reaching a low droplet actuation voltage of 36V, which was four instances decrease than the standard 150 V, minimizing the potential harm to the cells within the droplets and to the DMF chip. To exemplify the technological advances, drug sensitivity exams have been run in our DMF system to research the cell response of breast most cancers cells (MDA-MB-231) and breast regular cells (MCF-10A) to a broadly used chemotherapeutic drug, Cisplatin (Cis). The outcomes on-chip have been according to these screened in typical 96-well plates. This novel, easy and sturdy single-cell trapping technique has nice potential in organic analysis on the single cell degree.

3D Poly(Lactic Acid) Scaffolds Promote Totally different Behaviors on Endothelial Progenitors and Adipose-Derived Stromal Cells in Comparability With Commonplace 2D Cultures

Tissue engineering is a department of regenerative medication, which contains the mixture of biomaterials, cells and different bioactive molecules to regenerate tissues. Biomaterial scaffolds act as substrate and as bodily help for cells they usually also can reproduce the extracellular matrix cues. Though tissue engineering purposes in mobile remedy are likely to give attention to the usage of specialised cells from explicit tissues or stem cells, little consideration has been paid to endothelial progenitors, an vital cell kind in tissue regeneration.
We mixed 3D printed poly(lactic acid) scaffolds comprising two totally different pore sizes with human adipose-derived stromal cells (hASCs) and expanded CD133+ cells to judge how these two cell varieties reply to the totally different architectures. hASCs signify a really perfect supply of cells for tissue engineering purposes because of their low immunogenicity, paracrine exercise and talent to distinguish.
Expanded CD133+ cells have been remoted from umbilical twine blood and signify a supply of endothelial-like cells with angiogenic potential. Fluorescence microscopy and scanning electron microscopy confirmed that each cell varieties have been in a position to adhere to the scaffolds and preserve their attribute morphologies.
The porous PLA scaffolds stimulated cell cycle development of hASCs however led to an arrest within the G1 part and diminished proliferation of expanded CD133+ cells. Additionally, whereas hASCs maintained their undifferentiated profile after 7 days of tradition on the scaffolds, expanded CD133+ cells introduced a discount of the von Willebrand issue (vWF), which affected the cells’ angiogenic potential.
We didn’t observe adjustments in cell habits for any of the parameters analyzed between the scaffolds with totally different pore sizes, however the 3D atmosphere created by the scaffolds had totally different results on the cell varieties examined. In contrast to the extensively used mesenchymal stem cell varieties, the 3D PLA scaffolds led to reverse behaviors of the expanded CD133+ cells by way of cytotoxicity, proliferation and immunophenotype. The outcomes obtained reinforce the significance of finding out how totally different cell varieties reply to 3D tradition methods when contemplating the scaffold method for tissue engineering.

Cytotoxic and anti inflammatory results of chitosan and hemostatic gelatin in oral cell tradition

Chitosan is a biopolymer with bactericidal/bacteriostatic impact, biocompatible and biodegradable. It has been utilized in tissue engineering to exchange tissues partially or fully by releasing bioactive supplies or influencing cell progress, often in regenerative medication and dentistry. The purpose of this research was to judge the cytotoxic and anti inflammatory impact of chitosan alone or with hemostatic gelatin (Spongostand®) in cultures of human pulp cells (HPC), human gingival fibroblasts (HGF) and mouse pre-osteoblasts (MC3T3-E1, ATCC). HPC and HGF have been remoted from sufferers.
Cells have been subcultured in DMEM. Chitosan was inoculated at totally different concentrations (0-0.5%) and hemostatic gelatins impregnated with chitosan (0.19%) have been positioned instantly within the presence of cells and incubated for 24 hours. Cell viability was decided by MTT technique and imply cytotoxic focus (CC50) was calculated from the dose-response curve. Anti-inflammatory impact was calculated from the in vitro gingivitis mannequin induced with interleukin 1beta (IL-1β) in HGF and protein detection.
The information have been subjected to Shapiro-Wilk, Kruskal-Wallis and Mann-Whitney exams. Experiments have been carried out in triplicate of three impartial assays. Cell viability of HPC, HGF and MC3T3-E1 in touch with chitosan decreased considerably (p<0.05). The HPC have been probably the most delicate (CC50= 0.18%), adopted by HGF (CC50= 0.18%) and MC3T3-E1 (CC50= 0.19%).
The cytotoxicity of gelatins impregnated with chitosan decreased cell viability of HGF and HPC by 11% and 5%, respectively. The proinflammatory impact was diminished considerably within the gingivitis mannequin. To conclude, chitosan induces reasonable cytotoxic results alone or with hemostatic gelatin at 0.19%, in dose-dependent method, with anti-inflammatory results on human gingival fibroblasts. Using chitosan as a biomaterial could be a superb selection to be used in regenerative dentistry.
cellexusinc
cellexusinc

Cytotoxic and anti inflammatory results of chitosan and hemostatic gelatin in oral cell tradition

Chitosan is a biopolymer with bactericidal/bacteriostatic impact, biocompatible and biodegradable. It has been utilized in tissue engineering to exchange tissues partially or fully by releasing bioactive supplies or influencing cell progress, often in regenerative medication and dentistry. The purpose of this research was to judge the cytotoxic and anti inflammatory impact of chitosan alone or with hemostatic gelatin (Spongostand®) in cultures of human pulp cells (HPC), human gingival fibroblasts (HGF) and mouse pre-osteoblasts (MC3T3-E1, ATCC). HPC and HGF have been remoted from sufferers. Cells have been subcultured in DMEM.
Chitosan was inoculated at totally different concentrations (0-0.5%) and hemostatic gelatins impregnated with chitosan (0.19%) have been positioned instantly within the presence of cells and incubated for 24 hours. Cell viability was decided by MTT technique and imply cytotoxic focus (CC50) was calculated from the dose-response curve. Anti-inflammatory impact was calculated from the in vitro gingivitis mannequin induced with interleukin 1beta (IL-1β) in HGF and protein detection. The information have been subjected to Shapiro-Wilk, Kruskal-Wallis and Mann-Whitney exams. Experiments have been carried out in triplicate of three impartial assays. Cell viability of HPC, HGF and MC3T3-E1 in touch with chitosan decreased considerably (p<0.05). The HPC have been probably the most delicate (CC50= 0.18%), adopted by HGF (CC50= 0.18%) and MC3T3-E1 (CC50= 0.19%). The cytotoxicity of gelatins impregnated with chitosan decreased cell viability of HGF and HPC by 11% and 5%, respectively. The proinflammatory impact was diminished considerably within the gingivitis mannequin. To conclude, chitosan induces reasonable cytotoxic results alone or with hemostatic gelatin at 0.19%, in dose-dependent method, with anti-inflammatory results on human gingival fibroblasts. Using chitosan as a biomaterial could be a superb selection to be used in regenerative dentistry.

A Medium

DJ1018 100g
EUR 84.8

Advanced Medium

C0003-04 RT 500 mL Bottle
EUR 103

Medium 199

C0012-01 RT 500 mL Bottle
EUR 105

Heller's Medium

CP014-010 10X1L
EUR 99

Heller's Medium

CP014-500 50L
EUR 126

Hoagland's Medium

CP015-010 10X1L
EUR 99

Hoagland's Medium

CP015-500 50L
EUR 126

M9 Medium

SD7024 250g
EUR 71.75

M9CA Medium

SD7025 250g
EUR 71.75

YM Medium

SD7031 250g
EUR 70.45

TYGPN Medium

SD7032 250g
EUR 70.01

M63 Medium

SD7033 250g
EUR 71.75

NeuroProgenitor Medium

NM42400 125 ml
EUR 304

COLIFORM MEDIUM

C03-127-10kg 10 kg
EUR 1324

COLIFORM MEDIUM

C03-127-2kg 2kg
EUR 327

COLIFORM MEDIUM

C03-127-500g 500 g
EUR 125

HLP MEDIUM

H08-107-10kg 10 kg
EUR 2278

HLP MEDIUM

H08-107-2Kg 2 Kg
EUR 534

HLP MEDIUM

H08-107-500g 500 g
EUR 182

EC MEDIUM

E05-100-10kg 10 kg
EUR 814

EC MEDIUM

E05-100-2Kg 2 Kg
EUR 216

EC MEDIUM

E05-100-500g 500 g
EUR 95

DC MEDIUM

D04-117-10kg 10 kg
EUR 1579

DC MEDIUM

D04-117-2kg 2kg
EUR 382

DC MEDIUM

D04-117-500g 500 g
EUR 140

SIM MEDIUM

S19-110-10kg 10 kg
EUR 1021

SIM MEDIUM

S19-110-2kg 2kg
EUR 261

SIM MEDIUM

S19-110-500g 500 g
EUR 107

SOB MEDIUM

S19-124-10kg 10 kg
EUR 965

SOB MEDIUM

S19-124-2kg 2kg
EUR 249

SOB MEDIUM

S19-124-500g 500 g
EUR 104

50ml TC Tubes, Conical, 440 units/box

04-5540150 440 units/box
EUR 71

GMP IL4, 50µg

04-GMP-HU-IL4-50UG 50µg
EUR 483
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.

SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE

04-GSB
  • EUR 120.00
  • EUR 80.00
  • 1L
  • 500mL
Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You!

rHu IL 2 , 3MIU

04-RHIL2-02F01 1 vial
EUR 249
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

rHu IL 2 , 3MIU , Lot 200908F02

04-RHIL2-08F02 1 vial
EUR 249
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

PRE-GMP rHu GM-CSF, Molgramostim-Leukoma

04-RHUGM-CSF-7A10 300 µg
EUR 385
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.

Insect Cell Medium: TNM-FH Insect Culture Medium

ABP-MED-10001 1 liter Ask for price

Insect Cell Medium: Serum-Free Insect Culture Medium

ABP-MED-10002 1 liter Ask for price

Insect Cell Medium: Grace?s Insect Medium (Unsupplemented)

ABP-MED-10004 1 liter Ask for price

H Medium Broth

DJ1019 100g
EUR 60.88

Aqueous Mounting Medium

AR1018 10mL X5 (Enough for 800-1200 assays)
EUR 80

Antifade Mounting Medium

AR1109 10mL (for 1000 assays)
EUR 65

AddexBio Cryopreservation Medium

C0002-20mL RT vial
EUR 104

RPMI 1640 Medium

abx098879-500ml 500 ml
EUR 175

Recovery Medium - 6x12mL

1711 4/PK
EUR 128

Agarose, medium EEO

GE4750-100G 100 g
EUR 166

Agarose, medium EEO

GE4750-500G 500 g
EUR 429

Schwann Growth Medium

SGM001 500 ml
EUR 244

MCDB 131 Medium

CM034-050 500ml
EUR 85

MCDB 131 Medium

CM034-300 6x500ml
EUR 165

MCDB 131 Medium

CM034-310 10x500ml
EUR 219

MCDB 131 Medium

CM034-320 20x500ml
EUR 340

MCDB 131 Medium

CM034-350 50x500ml
EUR 585

Williams' Medium E

CM063-050 500ml
EUR 91

Williams' Medium E

CM063-300 6x500ml
EUR 266

Williams' Medium E

CM063-310 10x500ml
EUR 313

Williams' Medium E

CM063-320 20x500ml
EUR 604

Williams' Medium E

CM063-350 50x500ml
EUR 975

Cereal Crops Medium

CP003-005 5L
EUR 113

Cereal Crops Medium

CP003-010 10X1L
EUR 169

Kao Michayluk Medium

CP017-010 10X1L
EUR 99

Kao Michayluk Medium

CP017-500 50L
EUR 126

Orchid Maintenance Medium

CP037-010 10X1L
EUR 109

Orchid Maintenance Medium

CP037-500 50L
EUR 138

Woody Plants Medium

CP040-010 10X1L
EUR 101

Woody Plants Medium

CP040-500 50L
EUR 126

EverBrite Mounting Medium

23001 10mL
EUR 178
Description: Minimum order quantity: 1 unit of 10mL

Hepatocyte Maintenance Medium

HM42600 100 ml
EUR 409

Neuron Growth Medium

HNM001 500 ml
EUR 331

FluoreGuard Mounting Medium

FMM030 30 ml
EUR 96

FluoreGuard Mounting Medium

FMM060 60 ml
EUR 116

FluoreGuard Mounting Medium

FMM500 500 ml
EUR 327

FluoreGuard Mounting Medium

FMM999 1000 ml
EUR 530

CARY & BLAIR MEDIUM

C03-131-10kg 10 kg
EUR 2185

CARY & BLAIR MEDIUM

C03-131-2Kg 2 Kg
EUR 514

CARY & BLAIR MEDIUM

C03-131-500g 500 g
EUR 176

FLUID THIOGLYCOLLATE MEDIUM

F06-101-10kg 10 kg
EUR 792

FLUID THIOGLYCOLLATE MEDIUM

F06-101-2kg 2kg
EUR 211

FLUID THIOGLYCOLLATE MEDIUM

F06-101-500g 500 g
EUR 94

EC MEDIUM, MODIFIED

E05-108-10kg 10 kg
EUR 858

EC MEDIUM, MODIFIED

E05-108-2Kg 2 Kg
EUR 226

EC MEDIUM, MODIFIED

E05-108-500g 500 g
EUR 98

A-1 MEDIUM

A01-116-10kg 10 kg
EUR 1034

A-1 MEDIUM

A01-116-2Kg 2 Kg
EUR 264

A-1 MEDIUM

A01-116-500g 500 g
EUR 108

SuperMOUNT? Mounting Medium

1211-20
EUR 201

2XYT MICROBIAL MEDIUM

X24-102-10kg 10 kg
EUR 614

2XYT MICROBIAL MEDIUM

X24-102-2Kg 2 Kg
EUR 173

2XYT MICROBIAL MEDIUM

X24-102-500g 500 g
EUR 83