Cellexus Limited Cell Culture Biosystems

Electrospun polyurethane/poly (ɛ-caprolactone) nanofibers promoted the attachment and progress of human endothelial cells in static and dynamic custom conditions

• On this study, the angiogenic functionality of human endothelial cells was studied after being plated on the ground of polyurethane-poly caprolactone (PU/PCL) scaffolds for 72 hours. On this study, cells have been designated into 5 fully completely different groups, along with PU, PU/PCL (2:1), PU/PCL (1:1); PU/PCL (1:2); and PCL.

• Info revealed that the PU/PCL (2:1) composition had the subsequent modulus and breakpoint as in contrast with the alternative groups (p<0.05). As compared with the alternative groups, the PU/PCL scaffold with a molar ratio of two:1 had lower the contact angle θ and higher tensile stress (p<0.05).

• The indicate measurement of the PU nanofibers was lowered after the addition of PCL (p<0.05). Based totally on our data, the custom of endothelial cells on the ground of PU/PCL (2:1) did not set off nitrosative stress and cytotoxic outcomes beneath static conditions as compared with cells plated on a standard plastic flooring (p>0.05).

• Based totally on data from the static scenario, we fabricated a tubular PU/PCL (2:1) assemble for six-day dynamic cell custom inside loop air-lift bioreactors. Scanning electron microscopy confirmed the attachment of endothelial cells to the luminal flooring of the PU/PCL scaffold. Cells have been flattened and aligned beneath the custom medium motion. Immunofluorescence imaging confirmed the attachment of cells to the luminal flooring indicated by blue nuclei on the luminal flooring.

• These data demonstrated that the equipment of PU/PCL substrate could stimulate endothelial cells train beneath static and dynamic conditions.

Three-Dimensional CellCultures as an In Vitro Gadget for Prostate Most cancers Modeling and Drug Discovery

Inside the last decade, three-dimensional (3D) cell custom know-how has gained a variety of curiosity attributable to its potential to larger recapitulate the in vivo group and microenvironment of in vitro cultured most cancers cells.

Significantly, 3D tumor fashions have demonstrated a variety of fully completely different traits in distinction with typical two-dimensional (2D) cultures and have equipped an attention-grabbing hyperlink between the latter and animal experiments.

Definitely, 3D cell cultures characterize a useful platform for the identification of the natural choices of most cancers cells along with for the screening of novel antitumor brokers. The present consider is geared towards summarizing the commonest 3D cell custom methods and functions, with a consider prostate most cancers modeling and drug discovery.

Temperature responsive methylcellulose-hyaluronic hydrogel as a 3D cellculture matrix

• This study investigated the equipment of a temperature-responsive methylcellulose-hyaluronic acid (MC-HA) hydrogel to help 3D cell progress in vitro. Preliminary work centered on the preparation of hydrogels for 3D custom, adopted by investigations of the natural compatibility of hydrogel elements and optimisation of the cell custom environment.

• Evaluation of viability and proliferation of HCT116 cells cultured inside the MC-HA hydrogel was used to control the combination composition so to design a hydrogel with optimum properties to help cell progress.

• Two important factors in relation to utility of the proposed polymeric matrix in 3D cell custom have been demonstrated: i) 3D cultured cell aggregates will likely be launched/recovered from the matrix by means of a fragile course of that may shield cell viability, and ii) the hydrogel matrix is amenable to utility in 96-well plate format as an everyday methodology employed in in vitro tissue custom exams.

• The work on account of this truth reveals that MC-HA hydrogels present potential for in vitro 3D cell custom as low-cost and well-defined alternate choices to animal-derived or difficult synthetic strategies.

Mouse Primordial Germ Cells: In Vitro Custom and Conversion to Pluripotent Stem Cell Traces

Primordial germ cells (PGCs) are the embryonic precursors of the gametes. No matter a very long time of research, in vitro custom of PGCs stays a major problem and has beforehand relied on undefined elements akin to serum and feeders.

Notably, PGCs cultured for extended intervals do not preserve their lineage id nonetheless as an alternative bear conversion to variety pluripotent stem cell strains referred to as embryonic germ (EG) cells in response to LIF/STAT3 signaling. Proper right here we report every established and new methodologies to derive EG cells, in a wide range of varied conditions.

We current that major fibroblast progress subject simply is not required for EG cell conversion. We component the steps taken in our laboratory to systematically take away difficult elements and arrange a very outlined protocol that allows surroundings pleasant conversion of isolated PGCs to pluripotent EG cells.

In addition to, we present that PGCs can adhere and proliferate in custom with out the help of feeder cells or serum. This may successfully counsel novel approaches to establishing short-term custom of PGCs in outlined conditions.

Prigrow IV Medium
TM004	ABM	500ml	EUR 125

Prigrow XI Medium
TM011	ABM	450ml	EUR 125

Prigrow V Medium
TM015	ABM	500ml	EUR 145

Prigrow VI Medium
TM016	ABM	500ml	EUR 145

Prigrow IX Medium
TM019	ABM	500ml	EUR 145

Prigrow XV Medium
TM027	ABM	500 ml	EUR 145

Prigrow CI Medium
TM101	ABM	500ml	EUR 145

Prigrow X.1 Medium
TM010	ABM	1 Kit	EUR 385

Prigrow III Medium
TM003	ABM	500ml	EUR 125

Prigrow XII Medium
TM012	ABM	500ml	EUR 125

Prigrow XIV Medium
TM014	ABM	500 ml	EUR 195

Prigrow VII Medium
TM017	ABM	500ml	EUR 145

Prigrow XIII Medium
TM013	ABM	500 ml	EUR 125

Prigrow VIII Medium
TM018	ABM	500ml	EUR 145

Prigrow XVIII Medium
TM039	ABM	500 ml	EUR 195

Gentodenz
19-DENZ-500	Gentaur Genprice	500 g	EUR 416

Tryptone Growth Medium II
G010-500G	EWC Diagnostics	1 unit	EUR 43.24
Description: Tryptone Growth Medium II

ORCHID SEED SOWING MEDIUM II
P727	PhytoTechnology Laboratories	10L	EUR 110.7

HPAF-II Cells Complete Medium
CM-0611-125mL4	Elabscience Biotech	125 mL×4	EUR 98
Description: Complete Growth Medium

HPAF-II Cells Complete Medium
CM-0611	Elabscience Biotech	125mL×4	EUR 98
Description: Cell lines complete growth medium

HPAF-II Cells Complete Medium
MBS2568889-4x125mL	MyBiosource	4x125mL	EUR 155

Rye Agar A
765-M1854-500G	Gentaur Genprice	500 g	EUR 82

Rye Agar B
765-M1855-500G	Gentaur Genprice	500 g	EUR 93

Porcine Parvovirus Antibody Elisa Test Kit
767-LSY-30009	Gentaur Genprice	192 Wells/kit	EUR 382

QuantiChrom Hemoglobin Assay Kit
65-DIHB-250	Gentaur Genprice	250 tests	EUR 473

Malachite Green Phosphate Assay kit
65-POMG-25H	Gentaur Genprice	2500 tests	EUR 333

50ml TC Tubes, Conical, 440 units/box
04-5540150	Gentaur Genprice	440 units/box	EUR 85.2

GMP IL4, 50µg
04-GMP-HU-IL4-50UG	Gentaur Genprice	50µg	EUR 579.6
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.

SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE
04-GSB	Gentaur Genprice	Ask for price Ask for price	1L 500mL
Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You!

rHu IL 2 , 3MIU
04-RHIL2-02F01	Gentaur Genprice	1 vial	EUR 298.8
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

rHu IL 2 , 3MIU , Lot 200908F02
04-RHIL2-08F02	Gentaur Genprice	1 vial	EUR 298.8
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

PRE-GMP rHu GM-CSF, Molgramostim-Leukoma
04-RHUGM-CSF-7A10	Gentaur Genprice	300 µg	EUR 462
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.

Mouse Anti TNP Immunotoxicity
198-TNPG-1	Gentaur Genprice	100 µL	EUR 469.2

Exo-Check Exosome Antibody Array
322-EXO-FBS-50A-1	Gentaur Genprice	100 µg	EUR 469.2

1-Step Polymorphs, Human Cell Separation
71-AN221725	Gentaur Genprice	1	EUR 238.8

Monkeypox Virus Real Time PCR Kit
ZD-0076-01	Gentaur Genprice	25 tests/kit	Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit
ZD-0076-02	Gentaur Genprice	25 tests/kit	Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

GMP Recombinant Human Interleukin-4 (IL-4)
GMPhuIL-4-50ug	Gentaur Genprice	50ug	EUR 528

Hosta Multiplication Medium Stage II
30630166-3	Glycomatrix	25 L	EUR 61.84

Hosta Multiplication Medium Stage II
30630166-4	Glycomatrix	50 L	EUR 116.82

Pringsheim's Medium
M698-100G	EWC Diagnostics	1 unit	EUR 23.3
Description: Pringsheim's Medium

HOSTA INITIATION/MULTIPLICATION MEDIUM II
H3959	PhytoTechnology Laboratories	10L	EUR 100.06

BAT Medium (Alicyclobacillus Medium)
M1561-500G	EWC Diagnostics	1 unit	EUR 43.27
Description: BAT Medium (Alicyclobacillus Medium)

Cooked M Medium (R.C. Medium)
M149-100G	EWC Diagnostics	1 unit	EUR 27.32
Description: Cooked M Medium (R.C. Medium)

Cooked M Medium (R.C. Medium)
M149-500G	EWC Diagnostics	1 unit	EUR 130.49
Description: Cooked M Medium (R.C. Medium)

MSC Prime Medium, 25x
TBS8022-01	Tribioscience	1 mL	EUR 76

MSC Prime Medium, 25x
TBS8022-05	Tribioscience	5 mL	EUR 268

MSC Prime Medium, 25x
TBS8022-25	Tribioscience	25 mL	EUR 968

Lowenstein - Jensen Medium (L.J. Medium)
MM162-100G	EWC Diagnostics	1 unit	EUR 12.1
Description: Lowenstein - Jensen Medium (L.J. Medium)

Lowenstein - Jensen Medium (L.J. Medium)
MM162-500G	EWC Diagnostics	1 unit	EUR 34.85
Description: Lowenstein - Jensen Medium (L.J. Medium)

H-4-II-E [H4-II-E] Cells Complete Medium
CM-0088-125mL4	Elabscience Biotech	125 mL×4	EUR 98
Description: Complete Growth Medium

H-4-II-E [H4-II-E] Cells Complete Medium
CM-0088	Elabscience Biotech	125mL×4	EUR 98
Description: Cell lines complete growth medium

H-4-II-E [H4-II-E] Cells Complete Medium
MBS2568598-4x125mL	MyBiosource	4x125mL	EUR 155

ATCC Medium 1754: PETC Medium (Tetra Pac
M2104-500G	EWC Diagnostics	1 unit	EUR 63.72
Description: ATCC Medium 1754: PETC Medium (Tetra Pac

General Freeze Medium
PB180436-10mL10	Elabscience Biotech	10 mL×10	EUR 158
Description: Supplements & Reagents

General Freeze Medium
PB180436-10mL5	Elabscience Biotech	10 mL×5	EUR 98
Description: Supplements & Reagents

General Freeze Medium
MBS2567647-10x10mL	MyBiosource	10x10mL	EUR 195

General Freeze Medium
MBS2567647-5x10mL	MyBiosource	5x10mL	EUR 155

Human iPS Growth Medium (PSGro Medium)
TBS8023	Tribioscience	500 mL	EUR 189

Rat Cartilage Growth Medium PrimaCell: Normal Articular Cartilage Growth Medium
9-25023	CHI Scientific	5 x 100 ml	Ask for price

General Freezing Medium
PB180436	Elabscience Biotech	10mL×5	EUR 98
Description: Others

Medium 199
C0012-01	Addexbio	RT 500 mL Bottle	EUR 34

Medium 199
MBS2557648-10L	MyBiosource	10L	EUR 165

Medium 199
MBS2557648-50L	MyBiosource	50L	EUR 345

Medium 199
MBS2557648-5L	MyBiosource	5L	EUR 150

Medium 199
MBS2557648-5x50L	MyBiosource	5x50L	EUR 1060

Human Cartilage Growth Medium PrimaCell: Normal Articular Cartilage Growth Medium
9-46043	CHI Scientific	5 x 100 ml	Ask for price

HS Medium (Hydrosulphite of Sodium Medium)
77707	Sisco Laboratories	100 Gms	EUR 7.7
Description: Part D

HS Medium (Hydrosulphite of Sodium Medium)
77707-1	Sisco Laboratories	500 Gms	EUR 25.66
Description: Part D

SIM Medium (Sulfite Indole Motility Medium)
49810	Sisco Laboratories	100 Gms	EUR 6.84
Description: Part D

SIM Medium (Sulfite Indole Motility Medium)
49810-1	Sisco Laboratories	500 Gms	EUR 27.37
Description: Part D

Listeria Enrichment Medium Base (UVM Medium)
28685	Sisco Laboratories	100 Gms	EUR 11.97
Description: Part D

Listeria Enrichment Medium Base (UVM Medium)
28685-1	Sisco Laboratories	500 Gms	EUR 54.74
Description: Part D

MlO Medium (Motility Indole Ornithine Medium)
88736	Sisco Laboratories	100 Gms	EUR 7.7
Description: Part D

MlO Medium (Motility Indole Ornithine Medium)
88736-1	Sisco Laboratories	500 Gms	EUR 31.64
Description: Part D

Hosta Initiation/Multiplication Medium Stage I/II
30630165-3	Glycomatrix	25 L	EUR 61.84

Hosta Initiation/Multiplication Medium Stage I/II
30630165-4	Glycomatrix	50 L	EUR 116.82

Phyto Proteose Agar Medium Base (KB Medium)
PHM008-100G	EWC Diagnostics	1 unit	EUR 13.81
Description: Phyto Proteose Agar Medium Base (KB Medium)

Phyto Proteose Agar Medium Base (KB Medium)
PHM008-500G	EWC Diagnostics	1 unit	EUR 60.56
Description: Phyto Proteose Agar Medium Base (KB Medium)

Rat Lung PrimaCell 7: Normal Alveolar Epithelial Cells II Growth Medium
9-25096	CHI Scientific	5 x 100 ml	Ask for price