Category: Medium & Serums
Bacterial CellCultures in a Lab-on-a-Disc: a Easy and Versatile Device for Quantification of Antibiotic Remedy Efficacy
Pathogenic bacterial biofilms can be life threatening, tremendously decrease affected individual’s prime quality of life and are a substantial burden on the healthcare system. Current methods for evaluation of antibacterial therapies in clinics and in vitro strategies utilized in drug development and screening each do not facilitate biofilm formation or are cumbersome to operate, need…
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Analysis of Cryptococcal Extracellular Vesicles: Experimental Approaches for Studying Their Diversity Among Multiple Isolates, Kinetics of Production, Methods of Separation, and Detection in Cultures of Titan Cells
Extracellular vesicles (EVs) produced by members of the Cryptococcus genus are related to elementary processes of fungal physiology and virulence. Nonetheless, a number of questions in regards to the properties of cryptococcal EVs stay unanswered, largely due to technical limitations. We lately described a quick and environment friendly protocol of high-yield EV isolation from strong…
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Bioprocessing of Human Mesenchymal Stem Cells: From Planar Culture to Microcarrier-Based Bioreactors
Human mesenchymal stem cells (hMSCs) have demonstrated nice potential for use as therapies for a lot of kinds of illnesses. Because of their immunoprivileged standing, allogeneic hMSCs therapies are notably enticing choices and methodologies to enhance their scaling and manufacturing are wanted. Microcarrier-based bioreactor techniques present increased volumetric hMSC manufacturing in automated closed techniques than…
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Impact of human adipose tissue-derived stem cells on dermatofibrosarcoma protuberans cells in an indirect co-culture: an in vitro study
Background: Autologous adipose tissue switch could also be carried out for aesthetic wants following the resection of dermatofibrosarcoma protuberans (DFSP), the commonest cutaneous smooth tissue sarcoma, excluding Kaposi sarcoma. The regenerative effectiveness of cell-assisted lipotransfer relies on the presence of adipose tissue-derived stem cells (ADSCs). That is the primary research to guage the potential oncological dangers…
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A Scalable Method to Study Neuronal Survival in Primary Neuronal Culture with Single-cell and Real-Time Resolution
Neuronal loss is on the core of many neuropathologies, together with stroke, Alzheimer’s illness, and Parkinson’s illness. Totally different strategies had been developed to review the method of neuronal survival upon cytotoxic stress. Most strategies are based mostly on biochemical approaches that don’t enable single-cell decision or contain advanced and expensive methodologies. Introduced here’s a…
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Optimization of muscle cell culture media using nonlinear design of experiments
Optimizing media for organic processes, equivalent to these utilized in tissue engineering and cultivated meat manufacturing, is tough as a result of intensive experimentation required, variety of media parts, nonlinear and interactive responses, and the variety of conflicting design goals. Right here we exhibit the capability of a nonlinear design-of-experiments (DOE) technique to foretell optimum…
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Characterization of Endogenous and Extruded H 2 S and Small Oxoacids of Sulfur (SOS) in Cell Cultures
This report characterizes and quantifies endogenous hydrogen sulfide (H2S) and small oxoacids of sulfur (SOS = HOSH, HOSOH) in a panel of cell strains together with human most cancers (A375 melanoma cells, HeLa cervical cells) and noncancer (HEK293 embryonic kidney cells), in addition to E. coli DH5α and S. cerevisiae S288C. The methodology used is a translation of well-studied…
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Development of a Pig Mammary Epithelial Cell Culture Model as a Non-Clinical Tool for Studying Epithelial Barrier-A Contribution from the IMI-ConcePTION Project
The ConcePTION mission goals at producing additional information in regards to the dangers associated to the usage of treatment throughout breastfeeding, as this data is missing for mostly used medication. Taking into account a number of points, the pig mannequin has been thought of by the consortium as essentially the most applicable selection. The current…
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In vitro OP9-DL1 co-culture and subsequent maturation in the presence of IL-21 generates tumor antigen-specific T cells with a favorable less-differentiated phenotype and enhanced functionality
T cell receptor (TCR)-redirected T cells goal intracellular antigens reminiscent of Wilms’ tumor 1 (WT1), a tumor-associated antigen overexpressed in a number of malignancies, together with acute myeloid leukemia (AML). For each chimeric antigen receptor (CAR)- and TCR-redirected T cells, a number of scientific research point out that T cell subsets with a less-differentiated phenotype…
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Connection of ES Cell-derived Collecting Ducts and Ureter-like Structures to Host Kidneys in Culture
Work towards renal era typically goals both to introduce suspensions of stem cells into kidneys within the hope that they’ll rebuild broken tissue, or to assemble full new kidneys from stem cells with the goal of transplanting the engineered organs. In precept, there is likely to be a 3rd method; to engineer renal tissue ‘modules’…
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