Differentiation of Human Pluripotent Stem Cells Into Definitive Endoderm Cells in Various Flexible Three-Dimensional Cell Culture Systems: Possibilities and Limitations

• Robert Brown

The era of human stem cell-derived spheroids and organoids represents a serious step in fixing quite a few medical, pharmacological, and organic challenges. Because of the benefits of three-dimensional (3D) cell tradition programs and the varied functions of human pluripotent stem cell (iPSC)-derived definitive endoderm (DE), we studied the affect of spheroid dimension and 3D…