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Liquid chromatographic profiling of monosaccharide concentrations in complicated cell-culture media and fermentation broths
A steady part extraction, liquid chromatography and fluorescence (SPE-RPLC-FL) based totally protocol for the willpower of free monosaccharides in extraordinarily difficult raw supplies powders and formulated fermentation feedstocks and broths has been developed. Monosaccharides inside sample extracts have been derivatised pre-column with anthranilic acid and the derivatives separated using reversed-phase LC with fluorescence detection.…
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Production of Human Acid-Alpha Glucosidase With a Paucimannose Structure by Glycoengineered Arabidopsis Cell Culture
Plant cell cultures have emerged as a promising platform for the manufacturing of biopharmaceutics resulting from their cost-effectiveness, security, skill to regulate the cultivation, and secrete merchandise into tradition medium. Nevertheless, the usage of this platform is hindered by the era of plant-specific N-glycans, the lack to supply important N-glycans for mobile supply of biopharmaceutics, and low…
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Live-cell imaging of microglial interactions with radial glia in transgenic embryonic mouse brains using slice culture
Microglial dynamics and interactions with close by radial glia may be visualized in actual time in embryonic mouse mind tissue utilizing time-lapse imaging in slice tradition. This live-cell imaging protocol can be utilized to review the morphology and actions of a variety of cell varieties throughout quite a lot of mind areas and developmental time…
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