pureexo exosome isolation kit

pureexo exosome isolation kit

 

Cat. No. BL-P100 PureExo Cell
Producer 101Bio
Key Choices
  • Easy to utilize: No ultra-centrifugation (< 2 hours)
  • 10 fold larger yield (vs. totally different kits and ultracentrifuge)
  • Save worth (vs. antibodies-beads methodology)
  • Isolate Pure exosome (>95%)
  • Intact exosome (good morphology)
  • Use as little as 2 mL cell media to appreciate extreme yield of exosomes for any downstream functions: EM analysis, exosome label, exosome subpopulation, qRT-PCR profiling of exosomal miRNAs, and gel analysis of exosomal proteins
Particulars Product Dimension of 10 reactions (2 mL~4 mL medium / response). The yield of each response is 50~200 µL exosome, from which 150~400 µg exosomal protein or 50 ~ 200 ng exosomal RNA will probably be extracted.
Notes Storage: maintain all bottles upright , in cool and darkish place. Shelf Life: 12 months. On account of the exosome yield of this package deal is 10 fold larger than all totally different kits, you possibly can start with solely 2 mL medium. The utmost medium amount of each response is 4 mL from at most 5 x 105 cells. One PureExo® Column is only for one response. Do not exceed 4 mL medium or 5 x 105 cells to avoid imprecise layer separation and column clogging.
Storage Temp Ambient Temperature
Supply Conditions Ambient
FAQ 1 Could your package deal isolate/purify larger exosome-like vesicles as a lot as 1000 nm in diameter, from custom medium?
A: No, our package deal cannot isolate vesicles larger than 300nm.2, Does your P101 package deal work for plasma?
A: It actually works utterly for plasma.Three What’s the minimal amount of serum sample will probably be processed by your PureExo package deal (Cat.#: P101)?
A: 50 ul.4 Which package deal must I exploit for my urine and plasma samples?
A: Use DiagExo® package deal (Cat.#: P120) to isolate exosome from urine and Use PureExo® package deal for serum (Cat.#: P101) to isolate exosome from serum.5, Can I exploit your package deal to isolate exosome or endothelial microparticles from cultured cells and from plasma?
A: Our P100 package deal could be utilized to isolate exosome from each cell custom medium or serum. It isn’t for cells.

6 Is your exosome prep contaminated with IgG and/or beads?
A: We use specific reagents to grab exosomes. We did not use antibody or beads for the isolation. So the last word exosome prep simply is not contaminated with artificial IgG or beads.

7, Do your exosome prep incorporates Albumin?
A: Some sorts of cells akin to hepatocytes carries enriched Albumin, and these liver derived exosomes will probably be launched into the circulation system. So, if the exosomes from these kinds of samples are isolated, it’d incorporates Albumin.

8, Can I exploit your PureExo package deal (Cat. #P100) on our D.discoideum amoeba?
A: PureExo exosome isolation package deal captures launched exosomes or microvesicles from cells. For amoeba exosome isolation, we suggest to assemble amoeba custom medium because the similar technique to assemble cell custom medium.

9, What is the composition of the buffer that the last word exosomes prep could be in? Will it have an effect on my downstream assay?
A: The last word isolated exosomes are aggregated nano-particles blended with a extremely small amount of genuine custom medium or serum. PureExo Exosome Isolation package deal provides intact exosomes which allows for a varieties of downstream functions along with exosome ground labeling10, How have you ever learnt that an exosome prep is pure, free of totally different membrane-derived microparticles
(shredding vesicles)?
A: The isolated microvesicle from cell custom medium or serum using our isolation kits confirmed sphere membrane encapsulated particles with the diameters varied between 20-200nm beneath EM scanning. These characters help to search out out that the harvested microvesicle are exosomes. Shedding particles are reported to be irregular type with diameter as a lot as 1000nm. In addition to, the size distribution pattern is confirmed by dynamic mild scattering indicating the isolated microvesicles are of diameter between 100 and 250nm.

11, Are exosome functionally energetic?
A: Our collaborators used our kits to detect specific mirRNAs carried by exosomes. Luciferase train assay
confirmed energetic function of exosomal microRNA after the exosomes had been utilized to cells transfected with specializing in mRNA 3’UTR vector.

12, Is it very important to utilize glass tubes?
A: Using glass tubes as a substitute of plastic tubes is required in step Three and step 4.
Please uncover glass tubes from FisherScientific. http://www.fishersci.com (Search for Fisherbrand* Disposable
Borosilicate Glass Tubes Cat. NO: 14-961-26).

13, Why can’t I see clear fluff heart layer?
A: The following coping with errors may outcome within the failure:
1). Decision A/B/C did not mix totally sooner than together with the cell custom medium or serum.
2). Did not perform a low velocity centrifugation to remove cells and cell particles.
3). The ratio is off. For example: 0.125 mL A + 0.125 mL B + 0.5mL C to 2 mL medium. Overproportion of serum/medium amount to Decision mixture (A/B/C) will end in an indistinctive interphase.
4). The utmost starting amount is 4 ml medium, and it have to be from half million cultured cells or a lot much less. If cell amount or medium amount exceed this amount, the sample have to be splits into quite a few samples to proceed.
5). Cultured cells weren’t starve for 48 hours to remove contaminated exosomes derived from FBS.

14, What biomarker(s) do you use to find out / characterize the isolated exosomes?
A: We use exosome markers akin to flotillin, CD9, CD63, Alix, and TSG101 and plenty of others to characterize the isolated exosomes.

15, What is the yield of exosome using your kits?
A: For P100 package deal (PureExo package deal for cell custom medium), often, 50 ~ 100 ul exosomes will probably be isolated from 2ml cell custom media (As a lot as 4 ml of medium will probably be processed per response). If all the harvested exosomes are used for exosomal protein extraction (using our P200 package deal), the whole protein yield ranges from 150 ~ 200 ug / 2mL medium, 300 ~ 400 ug / 4 ml medium. If all the isolated exosome are used for RNA extraction (using our P200 package deal), complete exosomal RNA yield is 50 ~ 100 ng / 2ml medium, 100 ~ 200 ng / 4ml medium. For P101 package deal (PureExo package deal for serum or plasma), often 100 ~ 200 ul exosome will probably be isolated from 100ul to 600ul serum. If all the harvested exosomes are used for exosomal protein extraction (using our P200 package deal), the whole exosomal protein yield ranges from 300-400 ug protein. If all the isolated exosome are used for RNA extraction (using our P200 package deal), complete exosomal RNA yield is 100 ~ 300ng exosomal RNA For P120 package deal (DiagExo package deal for Urine), often 50 ~ 200 ul exosome will probably be isolated from Three mL urine. If all the harvested exosomes are used for exosomal protein extraction (using our P200 package deal), the whole exosomal protein yield ranges from 150-400ug protein. If all the isolated exosome are used for RNA extraction (using our P200 package deal), complete exosomal RNA yield is 50 ~ 200ng exosomal RNA

16, Can I exploit heparin or EDTA tube to assemble blood sample if I need to isolate exosome from plasma?
A: No. Heparin and EDTA will significantly impair the downstream assays. Use No-heparin-No-EDTA tube to assemble the blood sample. Centrifuge the sample immediately to assemble the plasma for exosome isolation. .

pureexo exosome isolation package
pureexo exosome isolation package deal

Software program

Isolate pure exosome from cell custom media (supernatant). This package deal yields lots larger purity than exosome precipitation methodology. This product is for evaluation use solely.

 

Description

 

P100 package deal is for exosome purification / isolation at extreme yield and purity from cell custom media.

  • Easy to utilize: No ultra-centrifugation (< 2 hours)
  • 10 fold larger yield (vs. totally different kits and ultracentrifuge)
  • Save worth (vs. antibodies-beads methodology)
  • Isolate Pure exosome (>95% purity vs. 25% purity by exosome precipitation methodology)
  • Intact exosome (good morphology).

 

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