Methylated DNA Quantification Kit

methylflash methylated dna quantification kit

Product Overview

The MethylFlash Methylated DNA Quantification Bundle (Fluorometric) is an entire set of optimized buffers and reagents to fluorometrically quantify worldwide DNA methylation by significantly measuring ranges of 5-methylcytosine (5-mC) in a microplate-based format. Compared with LUMA or LINE-1, Alu, and LTR-based assays, MethylFlash™ experience straight quantifies exact worldwide DNA methylation.

This gear will also be significantly optimized for paired use with the MethylFlash Hydroxymethylated DNA Quantification Bundle (Fluorometric) for concurrently quantifying every methylated DNA and hydroxymethylated DNA.

Product Choices
The MethylFlash™ Methylated DNA Quantification Bundle (Fluorometric) is a further refinement of our earlier SuperSense™ worldwide DNA methylation quantification kits by simplifying the workflow and enhancing the consistency of outcomes. It makes use of an progressive methodology to permit background alerts to be terribly low, eliminating the plate drying and blocking steps. Compared with chromatography-based methods akin to HPLC or mass spectrometry, this product affords an affordable resolution to exactly measure ranges of 5-methylcytosine. The gear has the subsequent advantages and choices:

  • Fluorometric assay with easy-to-follow steps for consolation and velocity. All the method may be accomplished inside 4 hours.
  • Progressive gear composition permits background alerts to be terribly low, which eliminates the need for plate blocking and permits the assay to be simple, right, reliable, and fixed.
  • Extreme sensitivity, of which the detection prohibit may be as little as 50 pg of methylated DNA and accepts as little as 20 ng of enter genomic DNA
  • Optimized antibody and enhancer choices allow extreme specificity to 5-mC, with no cross-reactivity to unmethylated cytosine and no or negligible cross-reactivity to hydroxymethylcytosine all through the indicated focus differ of the sample DNA.
  • Widespread optimistic and unfavourable controls are included, which can be acceptable for quantifying methylated DNA from any species.
  • Strip-well microplate format makes the assay versatile: information or extreme throughput analysis.

Principle & Course of
The MethylFlash™ Methylated DNA Quantification Bundle (Fluorometric) contains all reagents wanted for the quantification of worldwide DNA methylation. On this assay, DNA is definite to strip wells which may be significantly dealt with to have a extreme DNA affinity. The methylated fraction of DNA is detected using seize and detection antibodies after which quantified fluorometrically by finding out the absorbance in a fluorescence microplate spectrophotometer at excitation 530 and emission 590 nm. The amount of methylated DNA is proportional to the RFU (relative fluorescence fashions) measured, which may be calculated with the gear’s included formulation for relative methylation standing of two completely completely different DNA samples or absolute quantification of 5-methylcytosine (5-mC) using a typical curve.

Easy, Fast, and Versatile
All of the fluorometric assay has easy-to-follow steps for consolation and velocity, which may be achieved in 4 hours. The strip-well microplate format permits for a flexible assay in information or extreme throughput analysis. Widespread optimistic and unfavourable controls are included with the gear for quantifying methylated DNA from any species akin to mammals, vegetation, fungi, micro organism, and viruses.

Safe and Useful
The entire wished reagents, along with unfavourable controls and optimistic controls, for quantification of worldwide DNA methylation are conveniently packaged throughout the gear. The direct fluorometric quantification of DNA samples replaces outdated or inferior methods and eliminates the need for DNA digestion/denaturation, radioactivity, extraction, or chromatography.

Methylated DNA Quantification Kit
Methylated DNA Quantification Equipment

Extraordinarily Delicate and Explicit
The novel course of and proprietary gear compositions allow for proper quantification of methylated DNA to be achieved with extreme sensitivity and specificity. The detection prohibit of the enter DNA may be as little as 50 pg of methylated DNA.

MethylFlash (Fluorometric)Competitor ZCompetitor C
Assay PrincipleDirect ELISADirect ELISAIndirect ELISA
Format96-well plate96-well plate96-well plate
SensitivityWonderful detection prohibit: 0.05 ng of 5-mC DNAGood detection prohibit: >0.5 ng of 5-mC DNAVery poor detection prohibit: >1000 ng of 5-mC DNA
S/N Ratio>20 with very low background <4 with extreme background N/A*
Procedural ConsolationNo need for plate blocking and denaturation of DNARequires plate blocking and DNA denaturationRequires plate blocking, DNA digestion, and antibody aggressive incubation in an additional plate
Protocol Time <4 hr <4 hr >eight hr
DNA Type & SpeciesWidespread for any species, every ssDNA and dsDNAHuman and mouse ssDNA solelyQuite a few species
SpecificityExplicit to 5-mC solelyCross-reaction to 5-hmCN/A*
Quantitation TypeEvery relative and absolute quantificationRelativeRelative
Regular AdministrationRegular, quantitative in absolute amount of 5-mC, and may be universally used for any speciesUnstable, cannot be quantitative in amount, and will solely be used for human or mouseN/A*
Minimal Enter Amount20 ng100 ng1000 ng
Accuracy of DetectionExtreme, regular and quantified customary administration. Confirmed shut correlation with LC-MS analysis by clientsUnclear, unstable and un-quantified customary administrationN/A*
% 5-mC calculationStraightforwardToughNot Provided
Patented MethodPositiveNoNo
ReputeVery extreme printed citation relyLow printed citation relyLow printed citation rely
Assist ExpertiseSince 2006Since 2013Since 2011

 

Product Choices
The MethylFlash™ Methylated DNA Quantification Bundle (Colorimetric) is a further refinement of our earlier Methylamp™ worldwide DNA methylation quantification kits by simplifying the workflow and enhancing the consistency of outcomes. It makes use of an progressive methodology to permit background alerts to be terribly low, eliminating the plate drying and blocking steps. Compared with chromatography-based methods akin to HPLC or mass spectrometry, this product affords an affordable resolution to exactly measure ranges of 5-methylcytosine. The gear has the subsequent advantages and choices:

  • Colorimetric assay with easy-to-follow steps for consolation and velocity. All the method may be accomplished inside 4 hours.
  • Progressive gear composition permits background alerts to be terribly low, which eliminates the need for plate blocking and permits the assay to be simple, right, reliable, and fixed.
  • Extreme sensitivity, of which the detection prohibit may be as little as 0.2 ng of methylated DNA and accepts as little as 50 ng of enter genomic DNA.
  • Optimized antibody and enhancer choices allow extreme specificity to 5mC, with no cross-reactivity to unmethylated cytosine and no or negligible cross-reactivity to hydroxymethylcytosine all through the indicated focus differ of the sample DNA.
  • Widespread optimistic and unfavourable controls are included, which can be acceptable for quantifying methylated DNA from any species.
  • Strip-well microplate format makes the assay versatile: information or extreme throughput analysis.

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Robert Brown