Liquid chromatographic profiling of monosaccharide concentrations in complicated cell-culture media and fermentation broths

A steady part extraction, liquid chromatography and fluorescence (SPE-RPLC-FL) based totally protocol for the willpower of free monosaccharides in extraordinarily difficult raw supplies powders and formulated fermentation feedstocks and broths has been developed.   Monosaccharides inside sample extracts have been derivatised pre-column with anthranilic acid and the derivatives separated using reversed-phase LC with fluorescence detection.…

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