Tag: dh5 alpha protocol
Analysis of Cryptococcal Extracellular Vesicles: Experimental Approaches for Studying Their Diversity Among Multiple Isolates, Kinetics of Production, Methods of Separation, and Detection in Cultures of Titan Cells
Extracellular vesicles (EVs) produced by members of the Cryptococcus genus are related to elementary processes of fungal physiology and virulence. Nonetheless, a number of questions in regards to the properties of cryptococcal EVs stay unanswered, largely due to technical limitations. We lately described a quick and environment friendly protocol of high-yield EV isolation from strong…
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Bioprocessing of Human Mesenchymal Stem Cells: From Planar Culture to Microcarrier-Based Bioreactors
Human mesenchymal stem cells (hMSCs) have demonstrated nice potential for use as therapies for a lot of kinds of illnesses. Because of their immunoprivileged standing, allogeneic hMSCs therapies are notably enticing choices and methodologies to enhance their scaling and manufacturing are wanted. Microcarrier-based bioreactor techniques present increased volumetric hMSC manufacturing in automated closed techniques than…
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Impact of human adipose tissue-derived stem cells on dermatofibrosarcoma protuberans cells in an indirect co-culture: an in vitro study
Background: Autologous adipose tissue switch could also be carried out for aesthetic wants following the resection of dermatofibrosarcoma protuberans (DFSP), the commonest cutaneous smooth tissue sarcoma, excluding Kaposi sarcoma. The regenerative effectiveness of cell-assisted lipotransfer relies on the presence of adipose tissue-derived stem cells (ADSCs). That is the primary research to guage the potential oncological dangers…
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