Analysis of Cryptococcal Extracellular Vesicles: Experimental Approaches for Studying Their Diversity Among Multiple Isolates, Kinetics of Production, Methods of Separation, and Detection in Cultures of Titan Cells

Extracellular vesicles (EVs) produced by members of the Cryptococcus genus are related to elementary processes of fungal physiology and virulence. Nonetheless, a number of questions in regards to the properties of cryptococcal EVs stay unanswered, largely due to technical limitations. We lately described a quick and environment friendly protocol of high-yield EV isolation from strong…

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